FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6648180
Sequences flagged as poor quality0
Sequence length35-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG13300.012.9012611
CTATACT15600.012.7715764
ATTAGAC15300.012.6603723
TATACTG22650.012.2169235
TTTATAC26050.012.10983
TATATAC20250.012.0253443
TTATACT23700.011.9093614
CGACGGA9409.6351467E-411.440302120
GTATTAG15100.011.3633631
TAGGACA28650.011.2039184
TAATACT25300.011.1561994
GTATAGT13050.011.02771
GTAGGAC25200.010.9809353
TATACTT25250.010.9589435
ATAGTAC16750.010.9035773
CTAGACT16850.010.8387864
TCTATAC15150.010.5938843
CTGTCTA31750.010.584531120
TCTAGAC15850.010.4751893
ATAGGAC18000.010.4538513
Produced by FastQC (version 0.11.5)