FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6648180
Sequences flagged as poor quality0
Sequence length35-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142110.21375775024141946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG4107.5991393E-613.5135615
CTGTCTA35050.012.993839120
TATACTA14100.012.9672695
CTATACT17350.012.7742024
ATAATAC20750.012.0171183
TAATACT24150.011.9305244
GTATTAG15100.011.7550271
CCGTTAG5257.392611E-611.6220841
TACCCTG43700.011.4107655
GTATTAT21900.011.3977471
ATAGGGC16300.011.2184353
TAGGACA31300.011.1524314
TAGACTG21300.010.9250495
TTACTCT35100.010.8921914
TTATACT24500.010.8554664
TAGGACT23800.010.7091294
TTATACC19150.010.7054824
CTAATAC17650.010.6743183
GTAATAC16650.010.6498063
CGTTAGG5259.5028154E-510.5579752
Produced by FastQC (version 0.11.5)