FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20200839
Sequences flagged as poor quality0
Sequence length35-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434980.21532769010237643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG81900.013.5546385
CTATACT60650.012.5954944
TACCCTG121300.012.5951825
TATACCG12250.012.5615265
TAATACT93500.012.4610744
CTACCCT93150.012.3898954
TTATACT92550.012.2920734
TACACTG124450.012.0997425
CTAGACT53700.012.0764414
TAGGACT79850.011.7005214
TCTATAC58450.011.5663683
GTGTACG44350.011.5373211
TACACAG165000.011.4909425
GTATTAC63500.011.43709851
TAGACTG76150.011.3305295
GTACTAG32550.011.3250331
GTGTAGG107450.011.3162231
TAGTACT59000.011.2710554
CTACACT88550.010.9854014
TAACACT93850.010.8334964
Produced by FastQC (version 0.11.5)