FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17736495
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN864089548.71816556766148No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2262371.275545140119285TruSeq Adapter, Index 19 (97% over 38bp)
CTCCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCG237850.13410203086912043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCC850.0501.3205145
AACTCCC9500.0362.91766145
CCAACTG1600.0338.96103145
TCAAATT950.0326.2181145
TTCCCGG3000.0322.82145
TGACCTC3000.0322.82145
TGCACTC5300.0299.67444145
TAGACGC2650.0277.74704145
AACTCAC2550.0243.06448145
GCTCGGC1600.0242.115145
CTATATT651.789922E-7238.39017145
GACTCCC1150.0235.79898145
ATCCGAC350.0020000846221.36229145
CCGACTG2900.0203.76062140-144
GAGCCGA2350.0200.89076135-139
AGAACTC13800.0190.88489145
ATCGGAC2800.0179.85686145
CCAGTCC5050.0176.43233145
GGACCGC450.0042426917172.17067145
TTGGTCT1300.0165.09547130-134
Produced by FastQC (version 0.11.5)