FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004976204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26073004
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATGGGGAAATATAAACTTGAGATCGGAAGAGCACACGTCTGAACTCC3997761.533294744249646Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCATGGGGAAATATAAACTTGAGATCGGAAGAGCACACGTCTGAACTC1180990.45295509485596674Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCCCTATTGTTACTGTTGATGGATACGTGGACCCTTCTGGAGGAGATCG622030.238572433003884No Hit
GGACAACAAAAATGGATTGGAGATCGGAAGAGCACACGTCTGAACTCCAG604120.23170325904909153Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTATGTTCAGGAGACCCCACTCATGTTTAGCAGATGTACTTCTGTCAG521270.1999270970080778No Hit
GGGGAAATATAAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA488580.1873892245020942Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGCTGGACAACAAAAATGGATTGGAGATCGGAAGAGCACACGTCTGAA363240.13931651297257502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGCATGGGGAAATATAAACTTGAGATCGGAAGAGCACACGTCTGAACT338970.13000803436381939Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGTGAGGCTCCCCTTTCTTGCGGAGATTCTCTTCCTCTGTGCGCCGGT314970.1208031111413169No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG292280.11210062331137602No Hit
GGATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAAC291090.11164421253492691No Hit
ATCAGACGACACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAA290530.11142943099306854No Hit
TTGGCATGGGGAAATATAAACTTGAGATCGGAAGAGCACACGTCTGAACT285330.10943503096152633Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG261360.10024161389305197No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)