FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22735967
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1394300561.32576195241663No Hit
AGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTG1199510.5275825743413508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGCC1750.0227.91222130-134
CGCAAGT2750.0225.2492145
GTACTAG4700.0220.64537120-124
CCCGTGC1054.9112714E-11210.27792140-144
TTCACGC3650.0203.64998145
GTCACGT1451.8189894E-12175.57864135-139
GCAAATG69450.0161.1394145
GGCAGCT4150.0157.26692130-134
ATGCTAC1202.6593625E-8153.32765140-144
CCACAGC3700.0145.09294145
GTTGGCC4050.0142.75053145
CTCGCCA554.593676E-8139.92287125-129
CGCCCGC1300.0139.45677130-134
GTCATCC5250.0125.853516145
CCATCTC4000.0123.88706145
CATGGCC4150.0115.27285140-144
TAGCTCT2850.0114.852196135-139
ACCAGTT4000.0113.56314145
TCAGCCC1851.7689126E-7111.60996145
ACCAAGC2650.0111.349236125-129
Produced by FastQC (version 0.11.5)