FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004976216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27409332
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN882956232.213707360690144No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT2710090.9887471901905526TruSeq Adapter, Index 19 (97% over 38bp)
ACACAGGGAGGGGAACATCACACATCAGGGCCTGTAGGGGGATGAGGGAC388300.14166707893501382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)