FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17675730
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN859159448.60672798238036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTCG8100.0795.8658130-134
ACCATTT7750.0719.0931145
CTGATCC7400.0687.61694145
TACTGGT10550.0681.35785145
TTGTACG2300.0658.6512140-144
AGGGTTG7900.0622.117135-139
TGTACTC301.3951237E-4538.4513145
GCAGCCC6350.0534.3152130-134
TGACCTC950.0510.11182145
CACCTAT7900.0475.22827135-139
GAGGATA12950.0473.1491140-144
AAACTCC8150.0406.316145
TGACTAC454.701991E-4358.96753145
TCGGAAT20300.0353.06927135-139
AGACGTC703.8233648E-6346.1473145
AGAAGAC6800.0344.22928140-144
GAAGACT3600.0325.16742140-144
CGGCGCC506.446927E-4323.07077145
ACACTTC2000.0323.07077145
GAGAAGT12000.0312.8586135-139
Produced by FastQC (version 0.11.5)