FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20579077
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1344834765.34961213275017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGTT5900.0604.3573145
AAATTTT300300.0574.2799145
GTGTAAC8900.0477.87793130-134
ATTCCTT2250.0454.75702145
GCTTAGC3950.0447.43063145
ATACTGC4950.0419.67932145
AATTTGC26700.0400.64136145
ACGCCAC252.8660434E-6372.07388145
GCTCAGC14700.0371.23016145
ATTATTG4100.0343.53625130-134
CCAGTTT6150.0333.1972140-144
AGTGTGC7500.0314.1957145
TCAACAG7900.0313.4268140-144
TCTCGTG2900.0310.06158145
TTCATAC2850.0308.60303125-129
CGTATCG6250.0272.8542145
CTTTGAT12350.0268.63635145
CTTGAAG16250.0267.9674140-144
ACCTCGC2800.0265.76706145
GCGTTTT1300.0262.3598145
Produced by FastQC (version 0.11.5)