FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32543557
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2574998979.12469125609103No Hit
CAATGGTGTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAG571120.17549403096901792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCC2600.0129.04485145
CCTTACC2350.0124.92639145
TATATAG20250.0120.925995130-134
TCGCAGA77000.0101.653239
ACAGGGA346000.0101.321293
GCCGATG18550.0100.93989
TACGGCC31300.098.925944
GTGTCGC80250.098.66256
CAGGGAG354750.098.0340964
TGTCGCA82350.097.6751867
CACAGGG358800.097.353872
GACCATG14150.096.915889
CGGGCAT21550.096.188538
GTCGCAG81600.095.79358
CAATGGT83700.095.7301561
CGGCCAG32650.095.6433946
GTGGCAT168400.095.466488
ACACAGG341500.095.180381
CGCTCTG8950.094.881359
TCAGACG20950.094.671722
Produced by FastQC (version 0.11.5)