FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44848361
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3876227686.42963786346618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTC600.098.4541554
TACGCAC253.7372774E-798.4541552
ACAGGGA620500.095.57433
ACACAGG604350.094.063761
AGTTACG1050.093.765864
CACAGGG633150.093.758092
TACTGGC9850.093.456486
CAGGGAG638900.092.760184
GTGGCAT229900.092.5375068
TGTTACT25950.091.807899
CTGGCAT160500.091.423748
ACTGGCA37300.091.320717
TCGACCC1200.090.249646
TTGTTAC26250.090.196118
TCTGGCA45150.089.943187
ACCGATG11500.089.886299
TTTGGCA62100.089.4107067
AGTGGCA51050.089.383227
TGCCCTA26900.089.304141
TGCGCAT9050.089.20058
Produced by FastQC (version 0.11.5)