FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20347401
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1263901262.116100233145254No Hit
AAGGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTC1222670.6008973824224528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACCC1600.0862.2976145
ATCCCCC1150.0859.55493130-134
CCTAATT2250.0843.1354145
AGCCTTC700.0739.11224145
TGATATC8300.0589.5201130-134
TGGACTC4850.0580.79144145
TGTATCC4650.0571.7809135-139
CCTGGCC3650.0446.85083130-134
CCATGGA6150.0440.63605135-139
GAGTCCC6200.0398.58395130-134
ACCGACC150.008250838383.24335145
TGATGTT2000.0373.66226145
ATACTGG3800.0364.17987130-134
GATATCG6500.0351.808130-134
CGGAATA11100.0334.46265135-139
ACGTCTT3100.0333.79263145
TGAGCGC703.615969E-8328.49432145
ATAGCCC358.2752894E-4297.2375140-144
ATACCAC906.002665E-10288.9809140-144
GAATTTG5000.0286.3303135-139
Produced by FastQC (version 0.11.5)