FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004976230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6457200
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN363993456.37016044105805No Hit
ATCAGACGACACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAA4668127.229325404199963No Hit
GAATTTGTGCCGGTGTGGTGTCTGATGATCCCAGCGCTGACAAATCTTGC1879902.9113237935947467No Hit
CCATCGTTGTCCACTGAAGGACATTCAATTCAAACCATCCAGCATCCACC1712842.6526048442049186No Hit
TACTTTCCCGCTCTGATAGCTGCAGGCATGCCCAGCCCCTGCCCCACTCA1694632.624403766338351No Hit
CCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGC917631.421095831010345No Hit
CACTATGTTCAGGAGACCCCACTCATGTTTAGCAGATGTACTTCTGTCAG682561.057052592454934No Hit
TTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG659841.021867063123335No Hit
CCTGGAGTCTTCCCGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGC561150.8690299200892028No Hit
CACTATGTTCAGGCGACCCCACTCATGTTTAGCAGATGTACTTCTGTCAG465420.7207768072848912No Hit
ATCAGACGACACATGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAA68860.10664064919779472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)