FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004976233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11484365
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN826926372.00452963659724No Hit
ATCAGACGACACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAA849100.7393530247427699No Hit
CCATCGTTGTCCACTGAAGGACATTCAATTCAAACCATCCAGCATCCACC304490.2651343805251749No Hit
CCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGC280610.2443408930315259No Hit
GAATTTGTGCCGGTGTGGTGTCTGATGATCCCAGCGCTGACAAATCTTGC257720.22440944710482466No Hit
TACTTTCCCGCTCTGATAGCTGCAGGCATGCCCAGCCCCTGCCCCACTCA244930.21327256665910566No Hit
CACTATGTTCAGGAGACCCCACTCATGTTTAGCAGATGTACTTCTGTCAG164190.14296828775469955No Hit
TTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG141140.12289752197879465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)