FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004976234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27432142
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1114014740.60983280124461No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1638050.5971279967856684TruSeq Adapter, Index 7 (97% over 35bp)
AAGGGAAAATGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTC323110.11778518790111249No Hit
ACACAGGGAGGGGAACATCACACATCAGGGCCTGTAGGGGGATGAGGGAC300210.10943731626936024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)