FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004976235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121031.502212965373598No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT270.7029419422025514TruSeq Adapter, Index 19 (97% over 38bp)
GTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCGAGGACGCGGGTGCC80.2082790939859412No Hit
GTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCC60.15620932048945588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)