FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24089925
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2062287285.60787134040476No Hit
CACTATGTTCAGGAGACCCCACTCATGTTTAGCAGATGTACTTCTGTCAG511480.21232112594788072No Hit
TGCCCTATTGTTACTGTTGATGGATACGTGGACCCTTCTGGAGGAGATCG405940.16851027971236937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC304.9221853E-9108.4181751
CTATGTT69850.0105.677513
TATGTTC69500.0105.585864
TTGTTAC51500.0104.391198
ACAGGGA150500.0104.251893
ATTGTTA53200.0103.095757
ATGTTCA73200.0102.2479255
TGTTACT53300.0101.882579
CAGACGA16550.0101.843733
TACGGCC12850.0101.644964
ACGGCCA12850.0101.644965
CACAGGG153750.0101.554712
GATACGG12900.0101.250982
ACTATGT75050.0101.243982
CAGGGAG152850.0101.159874
TGCCCTA55450.0100.8904951
CTGGCAT67300.0100.739938
GCCCTAT55900.0100.73392
CCATCGT9200.0100.168971
TGTCGCA34400.0100.0431067
Produced by FastQC (version 0.11.5)