FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18844131
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1177584662.49078824595308No Hit
GTCTTCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGCCGCCCA250020.13267791441271554No Hit
GTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCC213660.11338278215111114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAG6250.0348.655120-124
CACTAAC7300.0298.18658140-144
CATCTAG1850.0175.4302120-124
CGCACCC1051.967419E-8161.26051135-139
GACAGCC1657.2759576E-12154.76088145
CCCCACT904.1836756E-10154.54565120-124
ACTAACG7550.0153.26137140-144
TCCTACC951.8189894E-12148.8689125-129
TGATCTC4050.0144.11595145
GGTAACT5700.0142.59637115-119
TAGGAAG21900.0142.2627135-139
TCACCTC4550.0136.29648145
ACCCTTC1003.6379788E-12135.45883135-139
CCTGCCC1103.3178258E-6132.65218145
CTAACGA5850.0131.90102140-144
ACCCCTT1802.5465852E-11131.69608135-139
TCCCCTT1456.715709E-9125.79086145
CCAGGCA7350.0124.40096135-139
AGAGCTC6300.0124.17044140-144
CCCTCCG2700.0113.37302140-144
Produced by FastQC (version 0.11.5)