FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22887888
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1285346756.15837948874969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCC1750.0232.52992140-144
TGCTCCG8200.0229.9128145
ACCTCCC4750.0206.08379145
CGAAAGT3400.0170.61401145
CTTGCAC2800.0168.32903145
GTTCAAC5250.0165.73933145
GCCGGAC1553.6379788E-12163.73442145
GCGTGCA6400.0146.45631135-139
TTGTACC4200.0135.497135-139
AAAGACG4050.0134.27956145
AAACTGC4100.0132.642145
TGATATC11550.0129.70033130-134
GAGGCCG6050.0127.26311135-139
GTCGTAA4750.0126.15065130-134
GCGAAAC855.4005795E-8118.149635135-139
TGACTCC74650.0116.56149145
TGCGGTC5000.0116.01753145
AACGTTC2650.0115.16811140-144
ACGTCTT12300.0114.9564145
GAACTCC1148100.0114.88323145
Produced by FastQC (version 0.11.5)