FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20442894
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG6884450.010.26774140-144
TCTAATG295850.09.429053140-144
AAGGGGG681000.09.009337140-144
AGGGGGG652350.08.852815140-144
AAAGGGG773450.08.34417140-144
AAAAGGG858850.08.17716140-144
AAAAAGG1050450.07.1588993140-144
CTTGAAA1754700.07.041506140-144
TGAAAAA1751400.06.770938140-144
TTGAAAA1774650.06.769108140-144
CGGAGTC131700.06.66746043
CTGCTTG2033000.06.62758140-144
TCTGCTT2173250.06.4982295140-144
GTCTCGC192500.06.47100541
TCGCTCT210250.06.4358374
TTCTGCT2246500.06.3979497140-144
TTCGCTC44300.06.3364683
CGTCTTG49350.06.3066816140-144
GAAAAAA1884850.06.185285140-144
TCTCGCT209450.06.15147542
Produced by FastQC (version 0.11.5)