FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004976348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335077
Sequences flagged as poor quality0
Sequence length140-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA903306.765901891800998No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT889106.659540985276505No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA839066.28473114284794No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT504873.781579639226801No Hit
GCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGC333612.4988071849039417No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG330732.4772353954116504No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT324722.4322192652558616No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA269412.017936044138278No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC263581.9742681508257576No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGTGTCC257791.93089986570063No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC208981.5653029750343987No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC176181.3196242613721905No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC135561.0153721470746633No Hit
GTGTCCTTACCGTGACGTCCACCGACGAGTACCTGGACCTGTCGGCGCCT132780.994549378050854No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC125810.9423426513976348No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA124750.9344030344317219No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA120720.9042175095518835No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC120560.9030190768023117No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT104560.783175801845137No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC101390.7594318529942468No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC95490.7152396453537886No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGTCGCTTGAGCGGGAAGCGGG93480.7001843339372935No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG93180.6979372725318466No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT90470.677638817835975No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA86910.6509736891580036No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT83850.6280536628224439No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA82540.6182414946853253No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT75720.5671582987348296No Hit
TTGTCGGTGGTGTTAGCGCCCGCCGTCTTGAGCACGGTAACGTAGGGTGT74940.5613159390806673No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG74490.5579453469724968No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT72670.544313174446118No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTGAAACATTGACGGAGAAGTAG68500.5130790209104045No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT67080.5024429302579552No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC66680.4994468483840258No Hit
CGCTGCCGAAGACCAACTGCTCCTGCTGGCCGGGCTCTGGGCCCGGGACT65160.4880617372630942No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG62780.4702350501132145No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC61240.4587001348985864No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG58650.4393005047648937No Hit
TTGTCGGTGGTGTTAGCGCCCGCCGTCTACAAAGAGAGAGCAGAGCGATA56470.42297185855197866No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA54370.4072424287138494No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC53970.4042463468399201No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC53580.40132516701283893No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC52420.3926365295784438No Hit
TTCATGGATGCACTGGAGTCAGCAGACACTGTTACCTGTCTGCGCAGAGG52380.39233692139105086No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA51140.3830490675818698No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA51110.3828243614413251No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA50730.3799780836610922No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA50560.37870474886467226No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC48920.36642081318156183No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG47620.35668354709129135No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT47140.3530882488425761No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT46170.3458227502982974No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG46060.3449988277829668No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA46040.34484902368927034No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC45830.3432760807054574No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT44510.3333890105214905No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG44470.33308940233409756No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA40880.3061995675155815No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT39990.29953328534608864No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT39970.29938348125239217No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC38580.2889720967404876No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC37730.28260542275838774No Hit
CTCCATCTCTTTGTCGGTGGTATTAACTCCAGCAGTCTTCAAGATCTGGA34220.25631480431465753No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT33860.2536183306281211No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG33420.25032264056679876No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG32490.243356750209913No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGCTGTCGCTTGAGCGGGAAGC31470.23571674143139312No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGCTTGGGTCAGCAGGGGCAGG31150.2333198759322496No Hit
GAGTGCTGAGGTGTGGGGCGGGCCTTCTGGGGCACAGCCTGGGCACAGAG29290.21938809521847802No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTCGCTCGGCGTGGAGGTTC29130.2181896624689063No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC28810.21579279696976278No Hit
GTGGACCAGGAAGGACTCCACTTCCACAGGGGCTCCCCAGGGCTGGGCTT28740.2152684826418252No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC27590.2066547472542782No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGGGTTTGGTTTGGT23570.17654412442128806No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA23420.17542059371856455No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTCTATATTTAGCTCGCTGTT22770.17055196067342931No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGGCATACGGTTTGG22400.16778058494004466No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTT22350.1674060747058035No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACTCCTG19750.14793154252526258No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG19190.14373702790176146No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA18410.1378946682475992No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT17570.1316028963123475No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACTCCTGGC16130.1208170015662018No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCACTC15830.11856994016075477No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC15310.1146750337246466No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACTCCTGGCG15290.11452522963095013No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG15270.11437542553725366No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT14600.10935698839842195No Hit
TGGCCTTCATACACCTCCCCAAAGGCGCCATGGCCCAGACCCCGAATGAG14130.10583659219655496No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG14010.10493776763437614No Hit
AAATTGGGAAACTTCTCCTATGTCAGTGATTCTGTTCAAGGATTTCACAG13510.10119266529196444No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTC13500.1011177632451162No Hit
GGTTGTAGTCGGTCATGATGGTCGAGGTGCGGAGCTTGCTCAGCTTGTAC13390.10029384072978562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)