FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004976354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274773
Sequences flagged as poor quality0
Sequence length137-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA785816.164313175757567No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC580714.5553992750081775No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT531374.168349972897135No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA501653.9352104256993203No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC472053.7030122225682534No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC351652.758530342264858No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC289702.272561467806425No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT284152.22902430471935No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT224731.7629021010015116No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA207741.629623470217835No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG201461.5803597973913786No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT158471.2431232854790617No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG151071.185073734696295No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC148261.1630305944666228No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA148061.16146168768871No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC134791.057364722974208No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA125490.9844105578012713No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT117450.9213405053291841No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG116460.9135744167785167No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG111810.8770973341920483No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA109440.8585057888737838No Hit
TGGCCTTCATACACCTCCCCAAAGGCGCCATGGCCCAGACCCCGAATGAG108930.8545050765901067No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT107980.8470527693950217No Hit
GGTTGTAGTCGGTCATGATGGTCGAGGTGCGGAGCTTGCTCAGCTTGTAC103470.8116739215530923No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG99240.7784915432002404No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA97230.7627240300822186No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC94230.7391904284135293No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTTTAGGTCCT91720.719500648350726No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC90720.7116561144611628No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG90040.70632183141626No Hit
TGAGGAAATCCAGTTCGTCCTGTTCAGAGCACACTTCAGGCAGCGTCTTC87990.6902405369426556No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC87950.6899267555870731No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG83900.6581563933343426No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA81580.6399570747105563No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA77920.6112460806747554No Hit
GCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGC77220.6057549069520612No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA76180.5975965917069157No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC75130.5893598311228744No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT71180.5583739222591003No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT71180.5583739222591003No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT70080.5497449349805809No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA69900.5483329188804594No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA69720.5469209027803381No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA69210.542920190496661No Hit
CCAATGCAGCGAACAATGTTCTGGTGGTTGAATTTGCTGATGATCAGGGC65820.5163272206110421No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC63430.49757878461498634No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA57870.45396317618901555No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG56990.4470599863662001No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC56320.4418041486601928No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC54090.42431083808646713No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGTGTCC51160.4013263537900473No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT47960.3762238453434455No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC45500.35692629197512027No Hit
GCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTAGATGTCTCGGGCCAT41740.3274308445503631No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG41590.3262541644669286No Hit
CAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCT41110.3224887881999383No Hit
CAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGGGACCT40300.3161347157493922No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT35110.27542158486255985No Hit
GTGTCCTTACCGTGACGTCCACCGACGAGTACCTGGACCTGTCGGCGCCT34490.2705579738510307No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT31500.2471028175212371No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG31360.24600458277669826No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC28360.22247098110800903No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG28110.22050984763561826No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC26390.20701724934556975No Hit
CTGGCCCTTGAAGCACTACACAGGCCACTTCCTACAGGAAGCCTCCCTGG24200.1898377201274266No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC23610.1852094451325844No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCACTCCTGGCGGT22260.1746193243816742No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTGCAGGTGGG21980.17242285489259657No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG21430.16810836125333686No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGTCGCTTGAGCGGGAAGCGGG19790.1552433256744534No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT19790.1552433256744534No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG19510.15304685618537575No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC19150.15022282398513304No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCACTCCTGGCGGTT17230.13516131891717192No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTCTATATTTAGCTCGCTGTT15990.1254340968941137No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT15700.1231591820661404No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGAACTCCTGGCGG15510.12166872062712342No Hit
CAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGGGACCC15100.11845246173240256No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACTCCTGGCGGT14300.11217683462075209No Hit
TTGTCGGTGGTGTTAGCGCCCGCCGTCTACAAAGAGAGAGCAGAGCGATA14120.11076481852063072No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTGCAGAGATA13290.10425385539229337No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTGTGAAGAGAAGGGGTGGAA13240.10386162869781522No Hit
CGCTGCCGAAGACCAACTGCTCCTGCTGGCCGGGCTCTGGGCCCGGGACT12810.10048847912530309No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)