FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004976360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096654
Sequences flagged as poor quality0
Sequence length141-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT12826811.696305306869807No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT800527.299658780253389No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA766086.985612599780788No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA492744.493121805054283No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC231362.1096900207358016No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG199191.8163431674894723No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT197831.8039418084464198No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC171441.5633007311330647No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC146761.3382525390870776No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTATGGAAGTGATATTCCTTGAG140111.2776135408250917No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA125631.1455755416020004No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC124591.136092149392607No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT122861.1203168911981354No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG119311.0879456966372256No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA110621.0087046598106604No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT105930.9659382084048387No Hit
CGGGTCTCCAGATGTGCTGTTAGTGTCTTCCAGGTCATCAATTGTCTTTT102190.9318344710364437No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA96130.8765754741240173No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTCCCGTGGCTGTCATCAGTG94740.863900555690309No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG85460.779279517514184No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC81050.7390662870878144No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA73730.6723177957678539No Hit
CTCTGCCTTCTTTGCGCTCGGACATCTCGCCGGAGACGGAGCGCTCTACG71510.6520744008593413No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA69530.6340194810760732No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC68550.6250832076479911No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG68180.6217093084965722No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA65900.6009187948067486No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAAACAAGATAAAGTTATCCGTG65700.5990950655357113No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC65250.5949916746758777No Hit
CCTCCTCACCACTGATGACAGCCACGGGACCCTTCATTCCAAATTTGGAC64860.5914354025973553No Hit
CAAACTCGATGCAGTGCTCCAGGCGCAGCTCAGGCTCCTCCAGGCTCACC64400.5872408252739697No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC63570.5796723487991654No Hit
GTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATTGGGGA61310.5590642080364454No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT57320.5226808090792537No Hit
CAAAAGCAGCAAGTCCAACTGCTATGGATACCCCAAAAGTGTCTTCTTCT57320.5226808090792537No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC56180.5122855522343419No Hit
ACATATGCTCCTTCAGTTGAGGCTGGCATACCAGTGATGATCTCGTTTGA55310.5043523299053302No Hit
CACAAGGAGCAGCGTAGAAAGGAAGAGGCAGGCAAAGACGGCCAGGCCCA55210.5034404652698116No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG50680.46213299728081964No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA50320.45885028459295274No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC48440.44170722944520335No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA46200.421281461609587No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC44140.4024970501179041No Hit
ACATGGCCAGCCCATCCTCTGGAGCCAGCACAGCCGGGCGGTTGATCCCA40280.36729907518688665No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT39500.36018653102984166No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT38230.34860585015875567No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA38170.3480587313774445No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC36340.33137160854745434No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTCTATATTTAGCTCGCTGTT36050.3287272011044505No Hit
CTTGTCCCAGTGGTGGATGTAGATGTAGATGAAGAAGTATTTGCTCCTTC35670.3252621154894798No Hit
AGCTGCAGTTGAAAAAGTTCTGCTCCAACTGCAATTCCCGAAGACTCAGC35530.3239855049997538No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT34670.3161434691342939No Hit
AGTGATGCTTTGTTAATGCGAAGTTCTGACTTCCCTGGCTTTTTTTGTAT32610.29735905764261106No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG31990.29170549690239583No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA31770.28969939470425493No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC31570.28787566543321774No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAACTGGAAAGGGCTCCTTGAGG29550.2694559997957423No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGGGTCTTTTCAAACATAG29530.2692736268686386No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG28980.26425837137328634No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT27920.25459260623678936No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG27550.2512187070853706No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG27160.2476624350068481No Hit
CGGGTCTCCAGATGTGCTGTTAGTGTATAGAGGTCATGTCATTGAGGGCG25610.23352853315631003No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT23690.21602073215435313No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA23470.21401462995621223No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT21510.19614208310004796No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT20950.19103564114114388No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC20920.1907620817504883No Hit
GTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATCGGGGA20520.18711462320841396No Hit
CCGGCGCTTGATGTGGTGAACACAGGCATCACTGAAGTATTGTGGGTTCT20020.18255530003082102No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTTTTCTTGGTTTTGGACT19800.18054919783268014No Hit
CAGCCAGGGTGGGATCCTTCAGGGCCTTCACAGCCACAAGCATCTTGTCC19550.17826953624388367No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC19170.17480445062891303No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGTTTCAGAGAAATTATGT18790.17133936501394242No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACTAGCTCCAACTACC18370.16750953354476436No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTC18120.16522987195596786No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT17970.16386207500269No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC17960.16377088853913813No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTATGTGCAGCACATTAAGA16570.15109597010542977No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC16020.14608071461007757No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC15690.14307156131286625No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGGTATG15440.14079189972406975No Hit
CCGGCGCTTGATGTGGTGAACACCTGAGGGGGCTTGCAGGGTCAGACGGT14550.13267630446795434No Hit
CCAAGGACAAGATGCTTGTGGCTGTGAAGGCCCTGAAGGATCCCACCCTG14540.13258511800440248No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT14460.1318556262959876No Hit
TGGACAGCCTTCAAGACCTGGCCCTGTGCATCCGTAGGTGCAGTTTGGAT14400.13130850751467646No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA13670.12465189567539077No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT13350.1217339288417313No Hit
AAATTGGGAAACTTCTCCTATGTCAGTGATTCTGTTCAAGGATTTCACAG13340.12164274237817943No Hit
CTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTGCTGAAAATGACTGAATA12890.1175393515183458No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTT12750.11626274102861979No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCACTCCTGGC12650.1153508763931012No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT12650.1153508763931012No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTTGCTG12550.11443901175758261No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC12230.11152104492392313No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGAT11720.10687053528277833No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAA11520.10504680601174116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)