FastQCFastQC Report
Fri 9 Sep 2022
EGAF00004976366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012517
Sequences flagged as poor quality0
Sequence length141-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT866658.55936246008709No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT494954.888312986349859No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA409634.045660467923008No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTATGGAAGTGATATTCCTTGAG383663.7891709472532313No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC311503.0764915552035177No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC270632.672844011507955No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA265052.617733825703667No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTCCCGTGGCTGTCATCAGTG213252.1061374771979136No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC192131.8975483868418999No Hit
CCTCCTCACCACTGATGACAGCCACGGGACCCTTCATTCCAAATTTGGAC166041.6398737008860096No Hit
CAAACTCGATGCAGTGCTCCAGGCGCAGCTCAGGCTCCTCCAGGCTCACC155221.5330112975880898No Hit
CTCTGCCTTCTTTGCGCTCGGACATCTCGCCGGAGACGGAGCGCTCTACG146871.450543546429344No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAAACAAGATAAAGTTATCCGTG130641.290249941482464No Hit
CAAAAGCAGCAAGTCCAACTGCTATGGATACCCCAAAAGTGTCTTCTTCT121801.2029427654054203No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG121131.1963255925579521No Hit
GTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATTGGGGA120501.190103474805855No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT111621.1024012436334403No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC102911.016377996616353No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA91050.8992441608387809No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC89190.8808740989040184No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT77900.7693697982354865No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA77840.7687772155924296No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC77430.7647279008648744No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC76150.7520861378129948No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA75160.7423085242025566No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG74970.7404320124995433No Hit
AGCTGCAGTTGAAAAAGTTCTGCTCCAACTGCAATTCCCGAAGACTCAGC69460.6860131731121552No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT68790.6793960002646868No Hit
ACATATGCTCCTTCAGTTGAGGCTGGCATACCAGTGATGATCTCGTTTGA68000.6715936621311049No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG66800.6597420092699678No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG64510.6371251050599644No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA63420.6263598537110981No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGGGTCTTTTCAAACATAG59850.5911011864492152No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAACTGGAAAGGGCTCCTTGAGG59660.5892246747462018No Hit
CTTGTCCCAGTGGTGGATGTAGATGTAGATGAAGAAGTATTTGCTCCTTC52630.5197937417347067No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTTTTCTTGGTTTTGGACT52230.5158431907809944No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC46820.46241198913203435No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGTTTCAGAGAAATTATGT39970.3947588040497098No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT38960.384783662891586No Hit
AGTGATGCTTTGTTAATGCGAAGTTCTGACTTCCCTGGCTTTTTTTGTAT38640.3816232221286161No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA38130.3765862696626328No Hit
CAGCCAGGGTGGGATCCTTCAGGGCCTTCACAGCCACAAGCATCTTGTCC38100.3762899783411044No Hit
GTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATCGGGGA35900.35456194809568625No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA35420.34982128695123144No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA32140.31742676913078993No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT32070.31673542271389027No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG31880.3148589110108769No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC31100.3071553366511377No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTATGTGCAGCACATTAAGA31010.30626646268655244No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC29980.29609379398074304No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA28800.2844396686672915No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT28760.2840446135719203No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC28440.28088417280895034No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACTAGCTCCAACTACC27410.270711504103141No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT26920.2658720791848433No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA26510.2618227644572881No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC25410.25095874933457907No Hit
TGGACAGCCTTCAAGACCTGGCCCTGTGCATCCGTAGGTGCAGTTTGGAT24300.2399959704380272No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA24020.23723058477042855No Hit
CCAAGGACAAGATGCTTGTGGCTGTGAAGGCCCTGAAGGATCCCACCCTG21950.21678648358496697No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC21910.21639142848959575No Hit
GTCCTTCAAGTTTAATATCCCATGATTGTCTATGTTTGAAAAGACCCACG21450.2118482948928265No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT20900.20641628733147196No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTCTGGAAAGGGATGAGAG20550.20295955524697362No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT20310.20058922467474619No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA18780.18547836727679634No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC18130.17905872197701372No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC18020.17797232046474282No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT17930.17708344650015753No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC17720.17500940724945852No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTTGTAGAAGCTGGCCATT17610.1739230057371876No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC17180.1696761634619468No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA16580.16375033703137826No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG16120.15920720343460898No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG15830.15634305399316753No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG15330.15140486530102704No Hit
GCTTCAGCACGATGTCTCTCCTCTTAATGTGCTGCACATACGTGTCCGGC13820.1364915354507628No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT13750.13580018903386312No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCACTCCTGGCGGT13410.1324422207232076No Hit
TTCATGGATGCACTGGAGTCAGCAGACACTGTTACCTGTCTGCGCAGAGG13290.1312570554370939No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTGAAACATTGACGGAGAAGTAG13290.1312570554370939No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC13130.12967683505560892No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG13080.1291830161863949No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG12640.12483741013731128No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG10450.10320814366573598No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT10450.10320814366573598No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTAAGGTGGGAGTTTCATTTT10440.10310937989189317No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG10320.10192421460577947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)