FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004976370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026267
Sequences flagged as poor quality0
Sequence length140-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT852968.31128741350935No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA674786.575092056940347No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT523625.102181011374233No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC464184.522994503379725No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA346953.380699174776155No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT345253.364134284742665No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA335373.267863041489203No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT280022.7285297101046804No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTAT226382.2058587092832567No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA186201.814342661315233No Hit
ACCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTT184371.7965110443968286No Hit
TGGGAATGCCTGGTTTATTTGGGACTCCAGCTCCAACCAGCTGGAAGGCG183351.7865721103767342No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG179811.7520781628952309No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC152231.483337182234253No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC146561.4280884019460822No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTATGTATTGCATAGC136541.3304529912780982No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC135051.315934352366392No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT123871.206995840263791No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA121411.1830254699800344No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC118141.151162416797968No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC116411.1343052051756513No Hit
TTCTCCTGGTCTGTGGAACCCTTCTTCAAAGTCTTCACTGCTACTTTGAT91720.8937245375716065No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTCTATATTTAGCTCGCTGTT90940.8861241762621229No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG83880.8173311623583337No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG81030.7895606114198351No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC77300.7532152938757652No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA76290.7433738003852799No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA62920.613095812298359No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA57460.5598932831319725No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC53390.5202349875812045No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG53340.5197477849331607No Hit
GTCATCTTCCACCTTAAATTCTGGTTCTGTAAATTATTTGAAGTGCTCTT50070.487884731751094No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC47410.4619655508751621No Hit
GCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGC47010.45806792969081145No Hit
CAACTATAATAGTAAGTATGAAACTTGTTTCTGGTATCCAAAAATCATCT40750.39707015815572366No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT40620.39580343127080964No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC40420.39385462067863436No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC39640.38625425936915053No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC37990.370176571983704No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT36600.3566323383680855No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA35470.3456215585222949No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT34590.33704679191672343No Hit
GAGTGCTGAGGTGTGGGGCGGGCCTTCTGGCCCAGGTGCCCTGGCTGACC33880.33012851431450096No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT33210.32359999883071366No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGTGTCC31720.3090813599190074No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA29840.29076254035255933No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCACTCCTGGC29720.28959325399725416No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG29260.2851109896352509No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA27880.2716641965492411No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTACTCCTGGCG27250.2655254431838888No Hit
AAATTGGGAAACTTCTCCTATGTCAGTGATTCTGTTCAAGGATTTCACAG27120.26425871629897485No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA26830.2614329409403206No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG26470.257925081874405No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT25510.24857079103196342No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG24890.24252947819621992No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC23590.22986220934708024No Hit
GTGTCCTTACCGTGACGTCCACCGACGTGAGTGCTGGCTCTGGCCTGGTG21950.21388196249124253No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT19380.18883974638178952No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG19250.18757301949687558No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT18480.18007009871700053No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT17310.16866955675277484No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG16510.16087431438407354No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTGTGAAGAGAAGGGGTGGAA15830.1542483583706774No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTACTCCTGG15380.1498635345382829No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC15280.14888912924219525No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA15200.1481096050053251No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTTGATTCTTTAATCTTCTA13710.13359096609361892No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACACTCCTG12900.12569828319530882No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTACTGTAAAATAGCA12770.12443155631039486No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTT12520.1219955430701757No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC12270.11955952982995655No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC12250.11936464877073899No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCACT12210.11897488665230394No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG12170.11858512453386888No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA11180.10893851210260097No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGTCGCTTGAGCGGGAAGCGGG10940.10659993939199058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)