FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004976374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966440
Sequences flagged as poor quality0
Sequence length139-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT680227.038409006249741No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA657276.800939530648566No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT393454.071127022888126No Hit
GCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGC344113.5605935184801956No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC297903.0824469185878067No Hit
GAGTGCTGAGGTGTGGGGCGGGCCTTCTGGCCCAGGTGCCCTGGCTGACC257482.6642109184222504No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA249912.5858822068622986No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGTGTCC241562.4994826373080583No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC155551.6095153346301891No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC142471.4741732544182773No Hit
GTGTCCTTACCGTGACGTCCACCGACGTGAGTGCTGGCTCTGGCCTGGTG140121.4498572078970242No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA129901.3441082736641696No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT128451.3291047555978643No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC118971.2310127892057448No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA114871.188589048466537No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG112711.1662389801746615No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT98011.0141343487438435No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC86310.8930714788295188No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT86250.8924506435991887No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG82600.854683167087455No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG79320.8207441744960887No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA76900.7957038202061173No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA73740.7630064980754108No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGTCGCTTGAGCGGGAAGCGGG72800.7532800794669094No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC72600.7512106286991432No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT70890.733516824634742No Hit
GTGTCCTTACCGTGACGTCCACCGACACAGAAGAGTGACACCCGCCTCTG69170.7157195480319524No Hit
CGCTGCCGAAGACCAACTGCTCCTGCTGGCCGGGCTCTGGGCCCGGGACT68720.7110632838044784No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG68230.705993129423451No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA67880.7023715905798601No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA67780.701336865195977No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT60040.6212491204834237No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC59500.6156616034104548No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG57110.5909316667356483No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTAT57050.5903108315053185No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG55430.573548280286412No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC54200.5608211580646496No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA53660.5552336409916808No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC52650.5447829146144614No Hit
ACCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTT52320.5413683208476471No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC52310.5412648483092588No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGCTTGGGTCAGCAGGGGCAGG51250.5302967592400977No Hit
TGGGAATGCCTGGTTTATTTGGGACTCCAGCTCCAACCAGCTGGAAGGCG50470.5222259012458094No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA49900.5163279665576755No Hit
TTGTCGGTGGTGTTAGCGCCCGCCGTCTACAAAGAGAGAGCAGAGCGATA49740.5146724059434625No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA46430.4804229957369314No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC44930.46490211497868467No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG44670.46221182898058855No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT43630.4514506849882041No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC43050.4454492777616821No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA41430.4286867265427755No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTATGTATTGCATAGC41400.4283763089276106No Hit
TTGTCGGTGGTGTTAGCGCCCGCCGTCTGCTCGGGGCCCGTGAACGCTCA39910.4129589007077521No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG37610.38916021687844043No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT37000.38284839203675347No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA34960.3617399942055378No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT34110.3529448284425314No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT33990.3517031579818716No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG32460.33587185960845994No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC31580.3267662762302885No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT30380.31434957162369104No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTCTATATTTAGCTCGCTGTT28410.2939654815611937No Hit
TTCTCCTGGTCTGTGGAACCCTTCTTCAAAGTCTTCACTGCTACTTTGAT26210.27120152311576506No Hit
GTGTCCTTACCGTGACGTCCACCGACGAGTACCTGGACCTGTCGGCGCCT25230.26106121435371055No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGCTGTCGCTTGAGCGGGAAGC24990.258577873432391No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC22260.23032987045238196No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTT22220.22991598029882868No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG19320.1999089441662183No Hit
GTGTCCTTACCGTGACGTCCACCGACAAGAGTGACACCCGCCTCTGAGAC18310.1894582177889988No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTC15040.15562269773602086No Hit
GTCATCTTCCACCTTAAATTCTGGTTCTGTAAATTATTTGAAGTGCTCTT14880.15396713712180787No Hit
GAGTGCTGAGGTGTGGGGCGGGCCTTCTGGGGCACAGCCTGGGCACAGAG14860.15376019204503125No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA14840.15355324696825462No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT14760.15272546666114814No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA14680.15189768635404166No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT14300.1479657298952858No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT14220.1471379495881793No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCACTCCTGGC12470.12903025537022472No Hit
CAACTATAATAGTAAGTATGAAACTTGTTTCTGGTATCCAAAAATCATCT11480.11878647406978189No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTTGCTG10970.11350937461197799No Hit
AAATTGGGAAACTTCTCCTATGTCAGTGATTCTGTTCAAGGATTTCACAG10760.11133645130582343No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC10510.10874963784611563No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT10240.10595587930963123No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTTGAATGGCAA10080.10430031869541824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)