FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004976378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299534
Sequences flagged as poor quality0
Sequence length139-151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC241431.8578198030986492No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA200741.5447075644038557No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC194701.4982293652955598No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG194691.4981524146347844No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC174311.341326967974674No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT171951.3231666120316974No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG169131.3014665256930562No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA161611.2435996287900124No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC155921.199814702808853No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC149211.1481808094286106No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG130491.004129172457204No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG127930.984429803298721No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA126960.9769655892035146No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC113960.8769297301955932No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA110580.8509204068535337No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC91980.7077921778114308No Hit
GGCATGTTTGTTGGTGATTCCAAGGAGCTATGACCTGGACATAAAAATAA86140.6628529919186417No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA84670.6515412447846689No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT81740.628994701177499No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT79610.612604210432355No Hit
GACAAACTCGTCAGTTTGTTTCGGGTAAAATTGCTGAAAGGAATCAAAAA74710.5748983866524462No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG73370.5645869981085527No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTCTGCAGGGGAGGAGGAAAGGT73140.5628171329107203No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCTGGAAAAATGAAGTCAT69170.5322677205829167No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC68330.5258038650777894No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG67890.5224180360036751No Hit
TGCTACAGCCAACCTCACACGACTCCCGCTCACAAACGGTGGCATAAGTA65290.5024108642020909No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG64910.49948673909262864No Hit
ACCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTT63860.4914069197112196No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGGTATG62730.48271149504360794No Hit
GACAGCGGCTGCGATCACCGTGCGGCACAGCTCGTCGCACAGTGGATCTG62540.48124943248887675No Hit
CCATGCGACCATCTGCAGCCAGAACCTGTACACATCAAGAGGAATGTGTG62330.479633468612595No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTAAAGGAAAGA62320.47955651795181964No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT60270.46378163249287824No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA59000.454008898574412No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTAT57910.44562127654990175No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCTGTGGAAGA55360.42599885805219406No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACCTGGGGAAGAGAGCATG55050.423613387568159No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTGAGTCAGTGCGA54580.41999670651171883No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAGTATCCTT53480.41153213382643317No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG48810.37559617524435684No Hit
CCCACATCCACCGAGGCATTGGGCACCTGGACCTTCAGCGTGGGCACACC48690.37467276731505295No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA48530.37344155674264773No Hit
GAGAGAGACTCCAGAGCGTTGAAGGAGAGATTCCTGCGGGCGTGGAGACA46040.35428084220959205No Hit
GTCCGGCTTGGAGGATGGGCCGGTGAGGCGATCGCTCTGGTGGAGAGAGG44230.3403527726092584No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT44210.34019887128770776No Hit
TGGCCTTCATACACCTCCCCAAAGGCGCCATGGCCCAGACCCCTGTGCAA44120.33950631534072984No Hit
CTGGCCCTTGAAGCACTACACAGGCCACTTCCTACAGGAAGCCTCCCTGG44030.3388137593937519No Hit
CAGTGTCGGGACTTGAGCTCTCAATATTCCTAATCAAAGGTGCAGTTTCA44020.33873680873297657No Hit
CAGCCAGGGTGGGATCCTTCAGGGCCTAGGAACAATGCAGGACACAGGTG44000.33858290741142594No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGAGTGCTGGCGCCACCCTGCCGCC43640.33581268362351424No Hit
CCAATGCAGCGAACAATGTTCTGGTGGTTGAATTTGCTGCAGAGCAGAGA43280.3330424598356026No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC42690.3285023708498585No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAACTGAAAACCAAACACAAAAA42520.327194209616678No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC42330.32573214706194686No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA42300.32550129507962083No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTGAAACATTGACGGAGAAGTAG42140.3242700845072157No Hit
TGGAGTCAGCTGAGGTGTTGAGAAGGAGAAAGGAGCCTGGAAAGAGACAG42080.3238083805425637No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC41270.31757537701976246No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA40770.31372784398099623No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG39960.30749484045819503No Hit
CCCAGTAAGCATCCCTCGAGAAGTAGAGGCCCAATGCCACTGTGGGAAGT39750.3058788765819132No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTAAGGTGGGAGTTTCATTTT38390.2954135867164691No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCA38370.2952596853949185No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCTTGATAAAAACAGTAAAAA37900.29164300433847823No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAACTGTGAGGCAAAACA36930.2841787902432718No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT36390.2800234545614043No Hit
GGTTGTAGTCGGTCATGATGGTCGAGGTGCGGAGCTTGCTCAGCTTGTAC35930.2764837241657394No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC35770.27525251359333425No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA35690.2746369083071316No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT35500.2731748457524005No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT35410.27248228980542255No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCTGTAGGAAGTGAAAGAGA34680.26686489156882387No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTAAGGGCTGTT34200.26317125985160833No Hit
TGAGGAAATCCAGTTCGTCCTGTTCAGAGCACACTTCAGGCAGCGTCTGG34140.26270955588695644No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTGAGTCAGTTTT33910.260939690689124No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT33220.2556300950956266No Hit
CCTCCGGAAGGTCATCTCAGCTGAGGAGATGGGTGGCTTGTGGGCAAACT32970.2537063285762435No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG32850.2527829206469396No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT32650.2512439074314331No Hit
TGTCTTGATTTTACTTTCCCAAATCCAAACCATGAGAAATCTGAAAACCA32540.25039745016290454No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTATGTATTGCATAGC32120.24716552241034095No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTAGATT31540.24270238408537212No Hit
CGGGTCTCCAGATGTGCTGTTAGTGTCTGTAGGGACACAGGGCAGGAGGG31240.24039386426211243No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT30920.23793144311730205No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTGCAGGTGGG30880.23762364047420076No Hit
GAGTGCTGAGGTGTGGGGCGGGCCTTCTGGGGCACAGCCTGGGCACAGAG30470.23446866338241246No Hit
CCAGCTCGTACTTCTCCCGCTGCTCGCGGTCCAGGGCCCAGCGCGTGCTC30350.23354525545310856No Hit
GTGGACCAGGAAGGACTCCACTTCCACAGGGGCTCCCCAGGGCTGGGCTG30280.23300660082768132No Hit
CCCATCCATTTCGGGGTCATTGTTTGAGGCTGCATTGCCGCCTGAGTAGC28200.2170008633864139No Hit
TGCTACAGCCAACCTCACACGACTCCCGCTGTGGAAGACAGGGAGCATGA28010.21553880083168275No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTAAAACAGAGAGAAAGAA27230.20953664929120744No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA27150.20892104400500486No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTCGCTCGGCGTGGAGGTTC26810.20630472153864385No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTACTGTAAAATAGCA26680.20530436294856463No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT26440.20345754708995686No Hit
CAAAAGCAGCAAGTCCAACTGCTATGGATACCTGTGAGGAACCAGAAACA24480.18837521757799333No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTATGGGAAGAAGAAGGGGCACT24210.1862975497370596No Hit
ACCACAGACGCAATCACCACCACAGCATAGACCTAGAGAAAGGAAGGGAG24180.1860666977547336No Hit
GTGGTGTGCCAAGGTCAAGCTGCTGGCCAGTTCCACGTGGCTGGGGAGAC24170.1859897470939583No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA24110.18552804312930635No Hit
CAAACTCGATGCAGTGCTCCAGGCGCAGCTCAGGCTCCTCCAGGCTCACC23720.1825269673590687No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG23580.18144965810821417No Hit
CGAGATGGAGAGGTTCCGGTTGAGAACCAGCCCTGCAGGTAGGGGCACAA23260.1789872369634038No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC22900.17621701317549213No Hit
GAGTCATCATCATTGCTGATAACGGAGGCTGGGCCTGGAGATGGAAAAAT22790.17537055590696357No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT22660.17437019731688436No Hit
TTTTCCGGCCCAGAGCAAGGAAGTGTTATCATTCCAGCCACCTCCACCAC22630.17413934533455838No Hit
CTTGTCCCAGTGGTGGATGTAGATGTAGCTGAAGAGGAGAGAAAGGAGAA22540.17344678938758046No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTG22310.17167692418974803No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC21980.16913755238416234No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCC21960.16898365106261168No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCACTCCTGGCGGTT21960.16898365106261168No Hit
TGAGGTTGGAGATTCGAGAAATGTGATAGTTGGTGCAACTAAATTGAAAA21630.16644427925702598No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGGTGA21580.16605952595314938No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC21380.16452051273764287No Hit
CACAAGGAGCAGCGTAGAAAGGAAGAGGCAGGCAAAGACGGCCAGGCCCA21280.16375100612988963No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTGTGAAGAGAAGGGGTGGAA21180.1629814995221364No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT21140.1626736968790351No Hit
TTCTCCTGGTCTGTGGAACCCTTCTTCAAAGTCTTCACTGCTACTTTGAT20400.15697934798166113No Hit
TGCAGATGCTGCTGGTTCTCGATGTAGCTGTGGGGAGAGCGGATGGGAGT20230.1556711867484806No Hit
CCTCCTCACCACTGATGACAGCCACGGGACCTGCACACACCAAGAGAGAC20080.15451692683685075No Hit
AGAACAATGTTATGTCGCTTGATGTGCTGAACAACTGCAAAACAAAGACA20010.1539782722114235No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT19920.15328571626444557No Hit
GTGGTGTGCCAAGGTCAAGCTGCTGGCCCAGATTAGTTACACACGACTTT19660.15128499908428714No Hit
TGCTACAGCCAACCTCACACGACTCCCGACACTTTAAAGCACAGTTTTTC19400.1492842819041287No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT19280.14836087397482484No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTGTAAGATGAAGGAA18590.14305127838132745No Hit
CTCTGCCTTCTTTGCGCTCGGACATCTCGCCGGAGACGGAGCGCTCTACG18530.1425895744166755No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGTAAGTAT18520.14251262375590018No Hit
TTGTTATCCTCACCAGCGGGGTGTTGGAGTTCATGGAGGAGCTGGACTCA18450.14197396913047294No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTACAAGGACAACATAGAACT18150.1396654493072132No Hit
GCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTGGGGAAGGAGAGGAAA18070.1390498440210106No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTGAGGAAAGGAAAAAAACC17700.1362026695723236No Hit
CCGGAACCTCAAGAAGGAGGTCAGCCGGGTGCTGTGCAAGCGGTGCGGTA17550.13504840966069376No Hit
GTTAGGTGCGGCCAGATTTGCAGATGGCAAACCTATGGAACAAGAAAAGT17030.1310469753003769No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC16870.1298157647279717No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTGCAAGGAAGAGTGGGGTCA16640.12804589953013926No Hit
GTGGTGTGCCAAGGTCAAGCTGCTGGCCCTGAAATGAAGAAGGAGGTAGT16310.12550652772455356No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAACTCCTGGCGGTT16280.1252756757422276No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG16090.12381361318749644No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA16080.12373666252672112No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTCTGGAAAGGGATGAGAG16000.12312105724051853No Hit
TGGGAATGCCTGGTTTATTTGGGACTCCAGCTTTAGGGAAAAAAAGAAAA16000.12312105724051853No Hit
AGTGATGCTTTGTTAATGCGAAGTTCTGACTTCCTATAAGATCAGGACCA15990.1230441065797432No Hit
GGGACAAGCCGAAATCCGAAATCTTCATCTTCCGCCCCTCAGCTACCAGG15940.12265935327586658No Hit
ACATATGCTCCTTCAGTTGAGGCTGGCATACCAGTGATGATCTCTGAAAA15840.12188984666811334No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCTA15720.12096643873880945No Hit
GTCATCTTCCACCTTAAATTCTGGTTCTGTAAATTATTTGAAGTGCTCTT15630.12027388279183153No Hit
CCAGCAGTCCCGCATCATCATGTACCTGCGGCAGGACTGTAAGGTCAGGG15630.12027388279183153No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTGTGCAAAAGAA15550.11965827750562893No Hit
CCAAGGACAAGATGCTTGTGGCTGTGAAGGTAAACCCCAGAGGCATGCCG15110.11627244843151467No Hit
GTCCCATTCCAACAAGTGAAGGAGCTCTGCAGGGCCATCTTGGAGCCTGG15020.11557989248453676No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC14970.11519513918066016No Hit
CTGAGGTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG14790.11381002728670432No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA14430.11103980349879265No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTGCAGAGATA14420.11096285283801732No Hit
TCGTGTCCCCCAACAATGCTGCCCCCTGAGGGCAGACGTAGCCCACTCAG14360.11050114887336537No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGTAAGTACTGGGATGGAA14330.11027029689103941No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAA14090.10842348103243163No Hit
CAGCATGGAGCCGCTTTTCCATCTTTTCTGTGTTGGTCCAGTATGGTGCT14000.1077309250854537No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCACTCCTGGCGGT13950.1073461717815771No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT13750.10580715856607059No Hit
AGCTGCAGTTGAAAAAGTTCTGCTCCAACTGCCTGTCGGCAAAGAGAGAG13660.10511460261909268No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGCTTGGGTCAGCAGGGGCAGG13560.10434509601133944No Hit
CCGGCGCTTGATGTGGTGAACACCTGAGGGGGCTTGCAGGGTCAGACGGT13070.10057451363334857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)