FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004976380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871639
Sequences flagged as poor quality0
Sequence length139-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC135991.5601642423067348No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA135391.553280658621287No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC128041.468956758474552No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC116881.3409221019252235No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG116861.3406926491357087No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC90791.0416009380030036No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT83730.960604103304235No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCTGGAAAAATGAAGTCAT82480.9462633039595522No Hit
GGCATGTTTGTTGGTGATTCCAAGGAGCTATGACCTGGACATAAAAATAA82480.9462633039595522No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG79130.9078299617158021No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA78990.9062237921891977No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA70760.8118039693038058No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG63420.7275947955518283No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG59680.6846871239125372No Hit
CAGCATGGAGCCGCTTTTCCATCTTTTCTGTGTTGGTCCAGTATGGTGCT58140.6670192591198879No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC56700.6504986582748133No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA48540.5568819201527238No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA47640.5465565446245522No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA44960.5158098708295521No Hit
GACAGCGGCTGCGATCACCGTGCGGCACAGCTCGTCGCACAGTGGATCTG44610.511794447013041No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG42730.49022588479863793No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG42360.48598100819261186No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT42110.4831128483236753No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT41750.4789826981124066No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCTTGATAAAAACAGTAAAAA41610.47737652858580215No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTTGCTG40590.46567443632054095No Hit
GACAAACTCGTCAGTTTGTTTCGGGTAAAATTGCTGAAAGGAATCAAAAA38890.44617094921177225No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTAAAGGAAAGA38800.4451384116589551No Hit
CCATGCGACCATCTGCAGCCAGAACCTGTACACATCAAGAGGAATGTGTG37580.43114179149854476No Hit
TGGAGTCAGCTGAGGTGTTGAGAAGGAGAAAGGAGCCTGGAAAGAGACAG37170.4264380093134887No Hit
GGTTGTAGTCGGTCATGATGGTCGAGGTGCGGAGCTTGCTCAGCTTGTAC36730.4213900479441604No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTCTGCAGGGGAGGAGGAAAGGT35630.40877014452083943No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA34970.40119820246684695No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAGTATCCTT34860.39993621212451486No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA34820.39947730654548497No Hit
CCTCCTCACCACTGATGACAGCCACGGGACCTGCACACACCAAGAGAGAC34480.3955766091237312No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT34480.3955766091237312No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGGTATG33980.38984028938585813No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA33040.37905600827865665No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTGTGCAAAAGAA32620.3742374996988432No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACCTGGGGAAGAGAGCATG32590.37389332051457086No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT31800.36482993532873126No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC31550.3619617754597947No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTGAGTCAGTTTT31360.3597819739594029No Hit
TTGTTATCCTCACCAGCGGGGTGTTGGAGTTCATGGAGGAGCTGGACTCA31200.3579463516432835No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCTGTGGAAGA31020.35588127653764917No Hit
CTTTGTCCGTGGTGTTAACACCGGCGGCCTAGAAAACAAGGGAAGCAAAA30940.3549634653795895No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTAAGGTGGGAGTTTCATTTT30900.3545045598005596No Hit
GAGAGAGACTCCAGAGCGTTGAAGGAGAGATTCCTGCGGGCGTGGAGACA30730.35255421108968277No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAACTGTGAGGCAAAACA30170.3461295329832649No Hit
CCCAGTAAGCATCCCTCGAGAAGTAGAGGCCCAATGCCACTGTGGGAAGT28600.32811748900634324No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCA27670.31744793429389917No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTGAGTCAGTGCGA26450.30345131413348875No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCTGTAGGAAGTGAAAGAGA26060.2989769847379477No Hit
CAGCCAGGGTGGGATCCTTCAGGGCCTAGGAACAATGCAGGACACAGGTG25910.2972560888165858No Hit
CTGGCCCTTGAAGCACTACACAGGCCACTTCCTACAGGAAGCCTCCCTGG25780.29576464568473876No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAACTGAAAACCAAACACAAAAA25590.293584844184347No Hit
CACAAGGAGCAGCGTAGAAAGGAAGAGGCAGGCAAAGACGGCCAGGCCCA24720.2836036478404477No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT24670.28303001586666043No Hit
CCCACATCCACCGAGGCATTGGGCACCTGGACCTTCAGCGTGGGCACACC24210.2777526017078171No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC23410.26857449012722007No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGAT23290.26719777339013057No Hit
CGGGTCTCCAGATGTGCTGTTAGTGTCTGTAGGGACACAGGGCAGGAGGG23220.2663946886268283No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTTGAATGGCAA23130.2653621510740111No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTAAGGGCTGTT22310.2559545867038992No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC22310.2559545867038992No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG22100.2535453324139925No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTAAAACAGAGAGAAAGAA21940.2517097100978731No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC21850.2506771725450559No Hit
CGAGATGGAGAGGTTCCGGTTGAGAACCAGCCCTGCAGGTAGGGGCACAA21400.24551448478097013No Hit
CTTGTCCCAGTGGTGGATGTAGATGTAGCTGAAGAGGAGAGAAAGGAGAA21020.24115488178018651No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT20810.2387456274902798No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGGACCTGGTGGCCTT20760.2381719955164925No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT20510.23530383564755591No Hit
CCAATGCAGCGAACAATGTTCTGGTGGTTGAATTTGCTGCAGAGCAGAGA20490.23507438285804103No Hit
CCTCCGGAAGGTCATCTCAGCTGAGGAGATGGGTGGCTTGTGGGCAAACT20370.23369766612095144No Hit
CAAAAGCAGCAAGTCCAACTGCTATGGATACCTGTGAGGAACCAGAAACA20190.23163259101531714No Hit
AGTGATGCTTTGTTAATGCGAAGTTCTGACTTCCTATAAGATCAGGACCA19560.22440482814559698No Hit
GTCCGGCTTGGAGGATGGGCCGGTGAGGCGATCGCTCTGGTGGAGAGAGG19530.22406064896132458No Hit
TGAGGAAATCCAGTTCGTCCTGTTCAGAGCACACTTCAGGCAGCGTCTGG19510.22383119617180963No Hit
GTCCGGCTTGGAGGATGGGCCGGTGAGGCGATCGCTCCACAACATCCAGG18590.21327636785412307No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGTAAGTACTGGGATGGAA18580.21316164145936564No Hit
TGGCCTTCATACACCTCCCCAAAGGCGCCATGGCCCAGACCCCTGTGCAA17980.20627805777391786No Hit
CCCATCCATTTCGGGGTCATTGTTTGAGGCTGCATTGCCGCCTGAGTAGC17660.20260681314167908No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTG17590.20180372837837685No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA16890.19377288074535445No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTACAAGGACAACATAGAACT16770.1923961640082649No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGTAAGTAT16440.18861019298126863No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG16400.18815128740223877No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTAGATT16360.18769238182320894No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGAGTGCTGGCGCCACCCTGCCGCC15530.1781700910583395No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTGTAAGATGAAGGAA15480.1775964590845522No Hit
CCAGCTCGTACTTCTCCCGCTGCTCGCGGTCCAGGGCCCAGCGCGTGCTC15420.17690810071600743No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTGCAGAGATA15380.17644919513697757No Hit
CAAACTCGATGCAGTGCTCCAGGCGCAGCTCAGGCTCCTCCAGGCTCACC15100.17323685608376863No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTAT15040.17254849771522385No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC14970.1717454129519216No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG14650.1680741683196828No Hit
GGGACAAGCCGAAATCCGAAATCTTCATCTTCCGCCCCTCAGCTACCAGG14530.16669745158259325No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT14230.16325565973986939No Hit
TGTCTTGATTTTACTTTCCCAAATCCAAACCATGAGAAATCTGAAAACCA14080.16153476381850743No Hit
GTGGACCAGGAAGGACTCCACTTCCACAGGGGCTCCCCAGGGCTGGGCTG14010.1607316790552052No Hit
TGCAGATGCTGCTGGTTCTCGATGTAGCTGTGGGGAGAGCGGATGGGAGT13950.1600433206866604No Hit
AGCTGCAGTTGAAAAAGTTCTGCTCCAACTGCCTGTCGGCAAAGAGAGAG13700.15717516081772384No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCTA13370.15338918979072758No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTGTGAAGAGAAGGGGTGGAA13360.15327446339597012No Hit
GCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTGGGGAAGGAGAGGAAA13130.1506357563165485No Hit
CCGGCGCTTGATGTGGTGAACACCTGAGGGGGCTTGCAGGGTCAGACGGT12950.14857068121091416No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTGAGGAAAGGAAAAAAACC12860.147538143658097No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATCTGTTCCAAAAAGAAAGACA12540.1438668990258582No Hit
TGAGGTTGGAGATTCGAGAAATGTGATAGTTGGTGCAACTAAATTGAAAA12480.1431785406573134No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCC12410.14237545589401115No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAA12390.14214600310449624No Hit
CAGTGTCGGGACTTGAGCTCTCAATATTCCTAATCAAAGGTGCAGTTTCA12210.1400809279988619No Hit
CCAGCAGTCCCGCATCATCATGTACCTGCGGCAGGACTGTAAGGTCAGGG12150.13939256963031715No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTGCAAGGAAGAGTGGGGTCA12040.13813057928798506No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTGCAGGTGGG11920.1367538625508955No Hit
ACCACAGACGCAATCACCACCACAGCATAGACCTAGAGAAAGGAAGGGAG11900.13652440976138058No Hit
TTTTCCGGCCCAGAGCAAGGAAGTGTTATCATTCCAGCCACCTCCACCAC11870.13618023057710818No Hit
GTGGTGTGCCAAGGTCAAGCTGCTGGCCCTGAAATGAAGAAGGAGGTAGT11640.13354152349768653No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG11410.1309028164182649No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC11200.1284935621283582No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTACTGTAAAATAGCA11190.12837883573360073No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA11170.1281493829440858No Hit
CCAAGGACAAGATGCTTGTGGCTGTGAAGGTAAACCCCAGAGGCATGCCG10930.12539594946990668No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTCTGGAAAGGGATGAGAG10810.12401923273281715No Hit
GCTTCAGCACGATGTCTCTCCTCTTAATGTGCTGCACATCTGTAGGATGG10510.12057744089009326No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGGTGA10440.11977435612679102No Hit
AGAACAATGTTATGTCGCTTGATGTGCTGAACAACTGCAAAACAAAGACA10380.11908599775824626No Hit
TTGAACTCTGACAGCAGGTCTCGCAGCTCACTCGGGGAGGCGTTCTCTGA10230.11736510183688431No Hit
AAATTGGGAAACTTCTCCTATGTCAGTGATTCCTGAGAAATCCAGTGGTG10020.11495584754697759No Hit
ACCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTT9970.11438221557319027No Hit
GTTAGGTGCGGCCAGATTTGCAGATGGCAAACCTATGGAACAAGAAAAGT9910.1136938572046455No Hit
TTGAACTCTGACAGCAGGTCTCGAAGCTCACTCGGGGAGGCGTTCTCTGA9550.10956370699337684No Hit
CTCCAGCCGTCTCTTGCTGACTGCACAGGACAGGGGGCAGCCCGCCTCCT9370.10749863188774252No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA9190.10543355678210818No Hit
TGGGAATGCCTGGTTTATTTGGGACTCCAGCTTTAGGGAAAAAAAGAAAA9020.10348320807123132No Hit
CTCTGCCTTCTTTGCGCTCGGACATCTCGCCGGAGACGGAGCGCTCTACG8970.10290957609744401No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAACTCCTGGCGGTT8880.10187703854462685No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTAAAAATGACAAATAAAGAA8870.10176231214986937No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA8850.10153285936035446No Hit
ACATATGCTCCTTCAGTTGAGGCTGGCATACCAGTGATGATCTCTGAAAA8840.10141813296559699No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC8810.10107395378132461No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)