FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004976382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218881
Sequences flagged as poor quality0
Sequence length139-151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA430733.5338150319842545No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC219581.8014884143735115No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG192581.5799737628201602No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC191181.5684878179248014No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA184311.5121246454740045No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA163181.3387689200176227No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG144051.1818216872688965No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT143601.178129776409674No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC139981.1484304046088174No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC114940.942996075908969No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA113620.9321664707219162No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG110250.9045181605095164No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC110150.9036977358741337No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA101570.8333053021582911No Hit
CCATGCGACCATCTGCAGCCAGAACCTGTACACATCAAGAGGAATGTGTG95350.7822748898374822No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT91300.7490476921044794No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG87630.7189381079859314No Hit
GGCATGTTTGTTGGTGATTCCAAGGAGCTATGACCTGGACATAAAAATAA84370.6921922648724527No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAGCAAGATCGTCAAC78950.6477252496347059No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA75880.6225382133284546No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTCTGCAGGGGAGGAGGAAAGGT74740.6131853724850909No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGGTATG71540.5869317841528418No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC70910.5817631089499303No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC70530.5786454953354757No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA69580.5708514612993394No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTAAAGGAAAGA68800.5644521491433536No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTAAGGGCTGTT66410.5448440003577052No Hit
GACAGCGGCTGCGATCACCGTGCGGCACAGCTCGTCGCACAGTGGATCTG64260.5272048706969753No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA63680.5224464078117552No Hit
CAGCATGGAGCCGCTTTTCCATCTTTTCTGTGTTGGTCCAGTATGGTGCT63540.5212978133222193No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT62860.5157189258016165No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT62810.5153087134839249No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCTGTGGAAGA58530.48019453908954196No Hit
CTTTGTCCGTGGTGTTAACACCGGCGGCCTAGAAAACAAGGGAAGCAAAA57740.473713184470018No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA55090.45197193163237426No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT54010.44311134557024023No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC53890.44212683600778097No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTGAGTCAGTTTT51710.42424157895643627No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT51620.42350319678459175No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT51560.4230109420033621No Hit
TTGTTATCCTCACCAGCGGGGTGTTGGAGTTCATGGAGGAGCTGGACTCA50430.4137401436235367No Hit
ACCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTT48400.39708552352526616No Hit
CAGCCAGGGTGGGATCCTTCAGGGCCTAGGAACAATGCAGGACACAGGTG48000.3938038249837351No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCTGGAAAAATGAAGTCAT47930.3932295277389672No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTGAGTCAGTGCGA47520.3898657867338977No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCA46660.3828101348696058No Hit
TGGAGTCAGCTGAGGTGTTGAGAAGGAGAAAGGAGCCTGGAAAGAGACAG45980.3772312473490029No Hit
TGGCCTTCATACACCTCCCCAAAGGCTCCATGGCCCAGACCCCTGTGCAA45540.37362137895331865No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAACTGTGAGGCAAAACA45480.373129124172089No Hit
GTCCGGCTTGGAGGATGGGCCGGTGAGGCGATCGCTCTGGTGGAGAGAGG44840.3678784065056392No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAACTGAAAACCAAACACAAAAA44820.36771432157856265No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTTGCTG44680.36656572708902674No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACCTGGGGAAGAGAGCATG44490.3650069202817995No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG42880.3517980836521367No Hit
GAGAGAGACTCCAGAGCGTTGAAGGAGAGATTCCTGCGGGCGTGGAGACA42630.34974702206367975No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG42470.34843434264706724No Hit
CCTCCTCACCACTGATGACAGCCACGGGACCTGCACACACCAAGAGAGAC41840.34326566744415576No Hit
TGAGGAAATCCAGTTCGTCCTGTTCAGAGCACACTTCAGGCAGCGTCTGG41180.3378508648506294No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTAGATT39470.3238216035855838No Hit
CTGGCCCTTGAAGCACTACACAGGCCACTTCCTACAGGAAGCCTCCCTGG39470.3238216035855838No Hit
GACAAACTCGTCAGTTTGTTTCGGGTAAAATTGCTGAAAGGAATCAAAAA38960.31963743794513166No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGAT38480.31569939969529426No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT38470.315617357231756No Hit
GTCCGGCTTGGAGGATGGGCCGGTGAGGCGATCGCTCCACAACATCCAGG37290.3059363465342392No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGTAAGTACTGGGATGGAA37130.30462366711762673No Hit
CCCAGTAAGCATCCCTCGAGAAGTAGAGGCCCAATGCCACTGTGGGAAGT37020.3037212000187057No Hit
CCAATGCAGCGAACAATGTTCTGGTGGTTGAATTTGCTGCAGAGCAGAGA36990.3034750726280908No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA36740.3014240110396339No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT36080.29600920844610756No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTAAGGTGGGAGTTTCATTTT35430.2906764483161195No Hit
CGAGATGGAGAGGTTCCGGTTGAGAACCAGCCCTGCAGGTAGGGGCACAA35420.2905944058525812No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG35420.2905944058525812No Hit
CCTCCGGAAGGTCATCTCAGCTGAGGAGATGGGTGGCTTGTGGGCAAACT34200.28058522530091123No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAGTATCCTT33410.27410387068138725No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC33240.27270914880123653No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC32450.2662277941817126No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA32430.266063709254636No Hit
CCCATCCATTTCGGGGTCATTGTTTGAGGCTGCATTGCCGCCTGAGTAGC31730.26032073680695655No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC31050.2547418492863536No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA30940.25383938218743257No Hit
GGTTGTAGTCGGTCATGATGGTCGAGGTGCGGAGCTTGCTCAGCTTGTAC29330.2406305455577698No Hit
CGGGTCTCCAGATGTGCTGTTAGTGTCTGTAGGGACACAGGGCAGGAGGG29230.23981012092238702No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG28610.23472348818301378No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGGTGA28610.23472348818301378No Hit
CCAGCAGTCCCGCATCATCATGTACCTGCGGCAGGACTGTAAGGTCAGGG28600.23464144571947548No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTAT28300.23218017181332712No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC27890.22881643080825773No Hit
GTGGACCAGGAAGGACTCCACTTCCACAGGGGCTCCCCAGGGCTGGGCTG27440.22512451994903523No Hit
CAAACTCGATGCAGTGCTCCAGGCGCAGCTCAGGCTCCTCCAGGCTCACC27390.2247143076313438No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG26900.2206942269179682No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTTGAACGGCAA26640.21856112286597296No Hit
CACAAGGAGCAGCGTAGAAAGGAAGAGGCAGGCAAAGACGGCCAGGCCCA26390.216510061277516No Hit
AGCTGCAGTTGAAAAAGTTCTGCTCCAACTGCCTGTCGGCAAAGAGAGAG26210.21503329693382703No Hit
CCAAGGACAAGATGCTTGTGGCTGTGAAGGTAAACCCCAGAGGCATGCCG26100.21413082983490597No Hit
CAGTGTCGGGACTTGAGCTCTCAATATTCCTAATCAAAGGTGCAGTTTCA25830.21191568331937247No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTAAAACAGAGAGAAAGAA25810.21175159839229588No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC25650.21043891897568343No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG25370.20814172999661165No Hit
TTGAACTCTGACAGCAGGTCTCGCAGCTCACTCGGGGAGGCGTTCTCTGA24850.2038755218926212No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG24600.20182446030416423No Hit
CTTGTCCCAGTGGTGGATGTAGATGTAGCTGAAGAGGAGAGAAAGGAGAA24590.20174241784062596No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTG24510.20108607813231974No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTGCAGGTGGG24400.20018361103339866No Hit
GCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTGGGGAAGGAGAGGAAA23730.19468676597633403No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG23190.190256472945267No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTGAGGAAAGGAAAAAAACC22900.18787724150265694No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC22210.18221631151851575No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTACTGTAAAATAGCA21760.17852440065929323No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT21580.17704763631560425No Hit
CAAAAGCAGCAAGTCCAACTGCTATGGATACCTGTGAGGAACCAGAAACA21510.1764733390708363No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTCTGGAAAGGGATGAGAG21150.17351981038345826No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGCAGGACGACGATGCC20860.1711405789408482No Hit
TGTCTTGATTTTACTTTCCCAAATCCAAACCATGAGAAATCTGAAAACCA20720.1699919844513123No Hit
GTGGTGTGCCAAGGTCAAGCTGCTGGCCCTGAAATGAAGAAGGAGGTAGT20500.1681870502534702No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTGCAAGGAAGAGTGGGGTCA20500.1681870502534702No Hit
AGAACAATGTTATGTCGCTTGATGTGCTGAACAACTGCAAAACAAAGACA20260.16621803112855152No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCTGTAGGAAGTGAAAGAGA20180.1655616914202453No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCTTGATAAAAACAGTAAAAA20050.16449513939424767No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA19780.16227999287871417No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA19630.16104935592564No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCC19560.16047505868087203No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT19540.1603109737537955No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCACTCCTGGCGGTT19520.16014688882671893No Hit
GCTTCAGCACGATGTCTCTCCTCTTAATGTGCTGCACATCTGTAGGATGG19100.15670110535811124No Hit
TGAGGTTGGAGATTCGAGAAATGTGATAGTTGGTGCAACTAAATTGAAAA19020.15604476564980505No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTATGGGAAGAAGAAGGGGCACT18490.15169651508227627No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT18260.14980953842089587No Hit
TGCTACAGCCAACCTCACACGACTCCCGCTCACAAACGGTGGCATAAGTA18250.1497274959573576No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC17970.14743030697828582No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTATGTATTGCATAGC17900.14685600973351787No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGAGTGCTGGCGCCACCCTGCCGCC17640.14472290568152266No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTGTGAAGAGAAGGGGTGGAA17510.143656353655525No Hit
TTTTCCGGCCCAGAGCAAGGAAGTGTTATCATTCCAGCCACCTCCACCAC17250.1415232496035298No Hit
CCGGCGCTTGATGTGGTGAACACCTGAGGGGGCTTGCAGGGTCAGACGGT17140.14062078250460872No Hit
AGTGATGCTTTGTTAATGCGAAGTTCTGACTTCCTATAAGATCAGGACCA17030.1397183154056877No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTGTGCAAAAGAA16830.1380774661349221No Hit
GGGACAAGCCGAAATCCGAAATCTTCATCTTCCGCCCCTCAGCTACCAGG16820.13799542367138382No Hit
GAGTGCTGAGGTGTGGGGCGGGCCTTCTGGGGCACAGCCTGGGCACAGAG16680.13684682918184793No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTGCAGAGATA16560.1358623196193886No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT16240.1332369607861637No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC16230.13315491832262544No Hit
CCCACATCCACCGAGGCATTGGGCACCTGGACCTTCAGCGTGGGCACACC16220.13307287585908714No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAA16080.13192428136955126No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT16000.13126794166124503No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC15940.13077568688001534No Hit
TCGTGTCCCCCAACAATGCTGCCCCCTGAGGGCAGACGTAGCCCACTCAG15760.12929892253632636No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT15740.1291348376092498No Hit
TGCAGATGCTGCTGGTTCTCGATGTAGCTGTGGGGAGAGCGGATGGGAGT15610.12806828558325217No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT15500.12716581848433112No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTTTCGGGGGAAACAGAGAGTGG15480.12700173355725458No Hit
CCAGCTCGTACTTCTCCCGCTGCTCGCGGTCCAGGGCCCAGCGCGTGCTC15270.1252788418229507No Hit
TTCCGAGGGAATTCCCACTTTGGATCCTCCTATGTTGGAAATGAGGGAAG15110.12396616240633827No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTGTAAGATGAAGGAA14980.12289961038034067No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGTAAGTAT14720.12076650632834543No Hit
TGCTACAGCCAACCTCACACGACTCCCGCTGTGGAAGACAGAGAGCATGA14710.12068446386480713No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA14630.12002812415650092No Hit
CCGGAACCTCAAGAAGGAGGTCAGCCGGGTGCTGTGCAAGCGGTGCGGTA14610.11986403922942437No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTACAAGGACAACATAGAACT14490.11887952966696504No Hit
CGCTGCCGAAGACCAACTGCTCCTGCTGGCCGGGCTCTGGGCCCGGGACT13990.11477740649005112No Hit
GTCCCATTCCAACAAGTGAAGGAGCTCTGCAGGGCCATCTTGGAGCCTGG13580.11141366548498172No Hit
ACCACAGACGCAATCACCACCACAGCATAGACCTAGAGAAAGGAAGGGAG13440.11026507099544583No Hit
GTTAGGTGCGGCCAGATTTGCAGATGGCAAACCTATGGAACAAGAAAAGT13280.10895239157883337No Hit
TGGGAATGCCTGGTTTATTTGGGACTCCAGCTTTAGGGAAAAAAAGAAAA13270.10887034911529508No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG12630.1036196314488453No Hit
GTGACTGCAGTTTAGATAATGCTTAATATTCACTTCCCCGTGGCCTTCCA12410.10181469725100316No Hit
GAGTCATCATCATTGCTGATAACGGAGGCTGGGCCTGGAGATGGAAAAAT12300.10091223015208212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)