FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004976390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278515
Sequences flagged as poor quality0
Sequence length139-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATGTTTGTTGGTGATTCCAAGGAGCTATGACCTGGACATAAAAATAA294392.302593242942007No Hit
CCATGCGACCATCTGCAGCCAGAACCTGTACACATCAAGAGGAATGTGTG196051.5334196313692057No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG177091.3851225836224057No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCTGGAAAAATGAAGTCAT175291.3710437499755577No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG169111.322706421121379No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGGTATG168631.3189520654822195No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTAAAGGAAAGA160401.2545805094191307No Hit
TGGAGTCAGCTGAGGTGTTGAGAAGGAGAAAGGAGCCTGGAAAGAGACAG155271.2144558335256137No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTCTGCAGGGGAGGAGGAAAGGT150861.1799626910908358No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTAAGGGCTGTT149581.1699510760530771No Hit
GACAGCGGCTGCGATCACCGTGCGGCACAGCTCGTCGCACAGTGGATCTG141371.1057359514749534No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC128771.0071841159470165No Hit
GTCCGGCTTGGAGGATGGGCCGGTGAGGCGATCGCTCTGGTGGAGAGAGG128511.005150506642472No Hit
TGGCCTTCATACACCTCCCCAAAGGCGCCATGGCCCAGACCCCTGTGCAA128081.0017872297157249No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCTGTGGAAGA121920.9536063323465114No Hit
TGAGGAAATCCAGTTCGTCCTGTTCAGAGCACACTTCAGGCAGCGTCTGG118890.929906962374317No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTAAGGTGGGAGTTTCATTTT116690.9126994990281695No Hit
CAGCCAGGGTGGGATCCTTCAGGGCCTAGGAACAATGCAGGACACAGGTG113380.8868100882664653No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC113030.8840725372795782No Hit
GACAAACTCGTCAGTTTGTTTCGGGTAAAATTGCTGAAAGGAATCAAAAA104170.8147733894400926No Hit
CCCAGTAAGCATCCCTCGAGAAGTAGAGGCCCAATGCCACTGTGGGAAGT101190.7914650981803109No Hit
GAGAGAGACTCCAGAGCGTTGAAGGAGAGATTCCTGCGGGCGTGGAGACA99640.7793416580955249No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAACTGAAAACCAAACACAAAAA99120.7752744394864355No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTGAGTCAGTGCGA91820.7181769474742181No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCA91590.7163779853971209No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACCTGGGGAAGAGAGCATG91050.7121543353030665No Hit
CCAATGCAGCGAACAATGTTCTGGTGGTTGAATTTGCTGCAGAGCAGAGA90580.7084781954063895No Hit
CTTTGTCCGTGGTGTTAACACCGGCGGCCTAGAAAACAAGGGAAGCAAAA87180.6818848429623431No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA84750.662878417539098No Hit
CCTCCGGAAGGTCATCTCAGCTGAGGAGATGGGTGGCTTGTGGGCAAACT82820.6477827792399776No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAACTGTGAGGCAAAACA80260.6277595491644603No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT79860.6246309194651608No Hit
GTGGACCAGGAAGGACTCCACTTCCACAGGGGCTCCCCAGGGCTGGGCTG78120.6110213802732076No Hit
GGTTGTAGTCGGTCATGATGGTCGAGGTGCGGAGCTTGCTCAGCTTGTAC76950.6018701384027564No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT76260.5964732521714646No Hit
CTGGCCCTTGAAGCACTACACAGGCCACTTCCTACAGGAAGCCTCCCTGG73540.5751985702162274No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTGAGTCAGTTTT71770.5613543837968268No Hit
CGGGTCTCCAGATGTGCTGTTAGTGTCTGTAGGGACACAGGGCAGGAGGG70960.5550189086557452No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC69950.5471191186650137No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCTTGATAAAAACAGTAAAAA68920.5390628971893173No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTAGATT68490.5356996202625702No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA66560.5206039819634497No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAGTATCCTT65270.5105141511832086No Hit
CAAAAGCAGCAAGTCCAACTGCTATGGATACCTGTGAGGAACCAGAAACA64660.5057429908917768No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTGCAGGTGGG64620.5054301279218468No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG62970.4925245304122361No Hit
CACAAGGAGCAGCGTAGAAAGGAAGAGGCAGGCAAAGACGGCCAGGCCCA61750.4829822098293724No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTG60870.47609922449091335No Hit
ACCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTT60570.47375275221643864No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT60020.4694508863799017No Hit
AGCTGCAGTTGAAAAAGTTCTGCTCCAACTGCCTGTCGGCAAAGAGAGAG58910.46076893896434534No Hit
CCTCCTCACCACTGATGACAGCCACGGGACCTGCACACACCAAGAGAGAC58740.4594392713421431No Hit
CCCATCCATTTCGGGGTCATTGTTTGAGGCTGCATTGCCGCCTGAGTAGC58040.45396416936836875No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA57360.4486454988795595No Hit
GCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTGGGGAAGGAGAGGAAA57090.4465336738325323No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTAAAACAGAGAGAAAGAA56300.4403546301764156No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTGAGGAAAGGAAAAAAACC54830.4288569160314897No Hit
TTGTTATCCTCACCAGCGGGGTGTTGGAGTTCATGGAGGAGCTGGACTCA54290.4246332659374352No Hit
CAGTGTCGGGACTTGAGCTCTCAATATTCCTAATCAAAGGTGCAGTTTCA53800.4208006945557933No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC53780.4206442630708283No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA53430.41790671208394115No Hit
TGAGGTTGGAGATTCGAGAAATGTGATAGTTGGTGCAACTAAATTGAAAA53110.41540380832450147No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTACTGTAAAATAGCA50180.39248659577713213No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA50060.3915480068673422No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTATGGGAAGAAGAAGGGGCACT50030.39131335963989466No Hit
CCAGCAGTCCCGCATCATCATGTACCTGCGGCAGGACTGTAAGGTCAGGG49400.3863857678634979No Hit
TGTCTTGATTTTACTTTCCCAAATCCAAACCATGAGAAATCTGAAAACCA49370.3861511206360504No Hit
TGCAGATGCTGCTGGTTCTCGATGTAGCTGTGGGGAGAGCGGATGGGAGT49100.3840392955890232No Hit
CCCACATCCACCGAGGCATTGGGCACCTGGACCTTCAGCGTGGGCACACC48510.3794245667825563No Hit
GTGGTGTGCCAAGGTCAAGCTGCTGGCCCTGAAATGAAGAAGGAGGTAGT46790.36597145907556816No Hit
CGAGATGGAGAGGTTCCGGTTGAGAACCAGCCCTGCAGGTAGGGGCACAA46670.3650328701657783No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCTGTAGGAAGTGAAAGAGA46050.3601834941318639No Hit
GAGTGCTGAGGTGTGGGGCGGGCCTTCTGGGGCACAGCCTGGGCACAGAG45440.3554123338404321No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGTAAGTACTGGGATGGAA45260.35400445047574725No Hit
AGAACAATGTTATGTCGCTTGATGTGCTGAACAACTGCAAAACAAAGACA44970.35173619394375505No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTGCAAGGAAGAGTGGGGTCA44920.3513451152313426No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGGTGA44740.3499372318666578No Hit
TTTTCCGGCCCAGAGCAAGGAAGTGTTATCATTCCAGCCACCTCCACCAC44360.3469650336523232No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA44350.3468868179098407No Hit
CCAAGGACAAGATGCTTGTGGCTGTGAAGGTAAACCCCAGAGGCATGCCG43850.34297603078571626No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG43770.3423503048458563No Hit
CCGGCGCTTGATGTGGTGAACACCTGAGGGGGCTTGCAGGGTCAGACGGT43590.34094242148117154No Hit
CAAACTCGATGCAGTGCTCCAGGCGCAGCTCAGGCTCCTCCAGGCTCACC43100.33710985009952954No Hit
CTTGTCCCAGTGGTGGATGTAGATGTAGCTGAAGAGGAGAGAAAGGAGAA41960.3281932554565257No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG41670.3259249989245335No Hit
ACATATGCTCCTTCAGTTGAGGCTGGCATACCAGTGATGATCTCTGAAAA41540.3249081942722612No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT41040.32099740714813674No Hit
TGGGAATGCCTGGTTTATTTGGGACTCCAGCTTTAGGGAAAAAAAGAAAA40190.3143490690371251No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCC40180.3142708532946426No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTGTAAGATGAAGGAA38700.3026949234072342No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA38280.2994098622229696No Hit
ACCACAGACGCAATCACCACCACAGCATAGACCTAGAGAAAGGAAGGGAG38000.29721982143345993No Hit
CCGGAACCTCAAGAAGGAGGTCAGCCGGGTGCTGTGCAAGCGGTGCGGTA37320.29190115094465063No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTGCAGAGATA37010.28947646292769347No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTGTGAAGAGAAGGGGTGGAA36500.2854874600610865No Hit
CAGCATGGAGCCGCTTTTCCATCTTTTCTGTGTTGGTCCAGTATGGTGCT36290.28384492946895423No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTACAAGGACAACATAGAACT35790.2799341423448298No Hit
CCAGCTCGTACTTCTCCCGCTGCTCGCGGTCCAGGGCCCAGCGCGTGCTC35140.2748501190834679No Hit
TTCCGAGGGAATTCCCACTTTGGATCCTCCTATGTTGGAAATGAGGGAAG34350.26867107542735125No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC33450.26163165860392723No Hit
GTTAGGTGCGGCCAGATTTGCAGATGGCAAACCTATGGAACAAGAAAAGT33310.2605366382091724No Hit
CGCTGCCGAAGACCAACTGCTCCTGCTGGCCGGGCTCTGGGCCCGGGACT32170.25162004356616857No Hit
CTCCAGCCGTCTCTTGCTGACTGCACAGGACAGGGGGCAGCCCGCCTCCT31140.24356382209047214No Hit
GGGACAAGCCGAAATCCGAAATCTTCATCTTCCGCCCCTCAGCTACCAGG30860.24137378130096246No Hit
GTGACTGCAGTTTAGATAATGCTTAATATTCACTTCCCCGTGGCCTTCCA30680.23996589793627765No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTCTGGAAAGGGATGAGAG30290.23691548397946055No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTGTGCAAAAGAA30000.23464722744746835No Hit
AGTGATGCTTTGTTAATGCGAAGTTCTGACTTCCTATAAGATCAGGACCA29510.23081465606582638No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC29380.22979785141355402No Hit
GCTTCAGCACGATGTCTCTCCTCTTAATGTGCTGCACATCTGTAGGATGG27960.2186912159810405No Hit
TTCTCCTGGTCTGTGGAACCCTTCTTCAAAGTCTATACAACATAAAAACA27570.2156408020242234No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGCGGGC27490.21501507608436352No Hit
GAGTCATCATCATTGCTGATAACGGAGGCTGGGCCTGGAGATGGAAAAAT27280.2133725454922312No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG27140.21227752509747638No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTAT27080.21180823064258142No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTTTCGGGGGAAACAGAGAGTGG26650.20844495371583438No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGCTTGGGTCAGCAGGGGCAGG26630.2082885222308694No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAA26480.20711528609363206No Hit
GTCCCATTCCAACAAGTGAAGGAGCTCTGCAGGGCCATCTTGGAGCCTGG26370.20625491292632467No Hit
TTGAACTCTGACAGCAGGTCTCGAAGCTCACTCGGGGAGGCGTTCTCTGA26090.20406487213681496No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGTAAGTAT25810.20187483134730524No Hit
ACATGGCCAGCCCATCCTCTGGAGCCAGCACAGCCGGGCCTAGAGAGGAG25700.2010144581799979No Hit
CTCTGCCTTCTTTGCGCTCGGACATCTCGCCGGAGACGGAGCGCTCTACG25550.19984122204276056No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC25460.19913728036041814No Hit
TCGTGTCCCCCAACAATGCTGCCCCCTGAGGGCAGACGTAGCCCACTCAG25430.19890263313297069No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGAGTGCTGGCGCCACCCTGCCGCC25150.19671259234346097No Hit
TGCTACAGCCAACCTCACACGACTCCCGCTGTGGAAGACAGGGAGCATGA23700.1853713096835No Hit
CAGGACCTTGGCTGCATGAAGTTTTAACATGGTAAAACTCATTGTATTTC23550.18419807354626266No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA22810.17841010860255843No Hit
GTCATCTTCCACCTTAAATTCTGGTTCTGTAAATTATTTGAAGTGCTCTT22370.1749686159333289No Hit
GTCCTTCAAGTTTAATATCCCATGATTGTCTATGTTTGAAAAGACCCCTG21990.17199641771899432No Hit
TTGAACTCTGACAGCAGGTCTCGCAGCTCACTCGGGGAGGCGTTCTCTGA21930.17152712326409936No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT21500.16816384633735232No Hit
CTCCATCTCTTTGTCGGTGGTATTAACTCCAGCAGTCTAGAAGAGACAAC21360.16706882594259748No Hit
CCGATGTCATTCGCTGCAGTTCCATAATCTTAAGTGGGCAAAGGGGGAAT20690.16182837119627067No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT20130.15744828961725127No Hit
GTTGATGATTTCTAACCTTTTCTGGTTTGCGATGAAACTGTAACAAAGCA19850.15525824882774156No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCTA19740.15439787566043417No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATCTGTTCCAAAAAGAAAGACA18080.14141406240834092No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTAAAAATGACAAATAAAGAA17670.13820721696655885No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA17520.13703398082932153No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTGAGGGGAAATG17320.13546966597967172No Hit
TTGTCGGTGGTGTTAGCGCCCGCCGTCTACAAAGAGAGAGCAGAGCGATA16240.12702236579156287No Hit
CGTGTGCAGCTGCACACTGGCCGGGACTGTGGGCCAGAAAGAGCAACAGC16070.12569269816936054No Hit
TGCGTGGTGTAGATATGATCAAAAAGGGATTCAATTGCCATCCATTTAAC15700.12279871569750844No Hit
CAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCT15220.11904436005834894No Hit
GTTTGGAACCTGCAGCCGATTCCTGGCTTTTCATCTCTTTCAATTGGGGA14300.11184851174995993No Hit
GCCCGCCAACTGCACACACGACCTGTGAGTGGCATCCCTGGCCCTCCACT14000.10950203947548522No Hit
AAATTGGGAAACTTCTCCTATGTCAGTGATTCCTGAGAAATCCAGTGGTG13350.10441801621412343No Hit
GTGTCCTTACCGTGACGTCCACCGACGTGAGTGCTGGCTCTGGCCTGGTG13230.10347942730433354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)