FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004976404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149586
Sequences flagged as poor quality0
Sequence length140-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG13029911.334428220246245No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC446593.8847898286861535No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA392513.414359604240135No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC356403.1002465235310797No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC324612.8237121885617955No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT298872.5998054951956617No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC259902.2608138930014805No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC223981.948353581202276No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA177981.5482095293436071No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG165201.4370390731967857No Hit
TGGCCTTCATACACCTCCCCAAAGGCGCCATGGCCCAGACCCCGAATGAG156861.3644912168380616No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTTTAGGTCCT151101.3143862225183676No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG149101.2969886550462515No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA127541.1094428776968404No Hit
GGTTGTAGTCGGTCATGATGGTCGAGGTGCGGAGCTTGCTCAGCTTGTAC112810.9813097932647057No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC112240.9763514865351526No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC110700.9629553595816233No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA109920.956170308267498No Hit
CTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTGCTGAAAATGACTGAATA107800.9377288867470549No Hit
TGAGGAAATCCAGTTCGTCCTGTTCAGAGCACACTTCAGGCAGCGTCTTC101670.8844053424450193No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA97980.8523068304589652No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT91240.7936770280779342No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC86910.756011294500803No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA80910.7038185920844547No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA68200.5932570507991572No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC64960.5650729914943292No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG61600.5358450781411743No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT58560.5094007755835579No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG57080.496526575654192No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA56680.49304706215976885No Hit
CCAATGCAGCGAACAATGTTCTGGTGGTTGAATTTGCTGATGATCAGGGC53390.46442806366813794No Hit
GCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTAGATGTCTCGGGCCAT53100.46190541638468113No Hit
CAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGGGACCT50990.44355098270159865No Hit
TGGACAGCCTTCAAGACCTGGCCCTGTGCATCCGTAGGTGCAGTTTGGAT47170.41032162882985695No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC46920.4081469328958425No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT46860.407625005871679No Hit
CAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCT44570.3877047911161061No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA43370.37726625063283653No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT38030.3308147454822867No Hit
CTGGCCCTTGAAGCACTACACAGGCCACTTCCTACAGGAAGCCTCCCTGG37290.32437764551760373No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG37040.32220294958358925No Hit
CCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG35720.3107205550519926No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG34670.3015868321291317No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTGCAGGTGGG34400.29923816052039603No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG32780.285146130867982No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA32550.2831454106086887No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC31740.2760993957824817No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC29520.2567880958884329No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG28590.24869822701389893No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT27660.24060835813936496No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG27590.2399994432778409No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA27360.23799872301854755No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC24990.21738260556409No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT24590.21390309206966684No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT24540.21346815288286392No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT24080.20946671236427725No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT22540.19607058541074787No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTGAAACATTGACGGAGAAGTAG21570.1876327651867716No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACTTTAGACTCC21240.18476216655387245No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA20120.17501952876948745No Hit
TGCTACAGCCAACCTCACACGACTCCCGCTCACAAACGGTGGCATAAGTA19070.16588580584662652No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT18860.16405906126205433No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC18300.15918774236986186No Hit
CAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGGGACCC17540.15257666673045775No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG16860.14666149378993829No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA16750.1457046275789719No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCACTCCTGGCGGTT16590.14431282218120264No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCA16440.1430080046207939No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC16440.1430080046207939No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA16330.14205113840982753No Hit
GTGGTGTGCCAAGGTCAAGCTGCTGGCCCAGATTAGTTACACACGACTTT16040.13952849112637072No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC16010.13926752761428898No Hit
TGCTACAGCCAACCTCACACGACTCCCGCTGTGGAAGACAGGGAGCATGA15280.13291741548696662No Hit
TGCTACAGCCAACCTCACACGACTCCCGACACTTTAAAGCACAGTTTTTC15190.1321345249507214No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT14730.1281330844321347No Hit
TGAGGAAATCCAGTTCGTCCTGTTCAGAGCACACTTCAGGCAGCGTCTGG13520.11760755611150447No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTATGGAAGTGATATTCCTTGAG13390.11647671422581693No Hit
CAGTGTCGGGACTTGAGCTCTCAATATTCCTAATCAAAGGTGCAGTTTCA13100.11395406694236013No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTAT12950.11264924938195141No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT12450.10829985751392239No Hit
GGCATGTTTGTTGGTGATTCCAAGGAGCTATGACCAGTCCCGAAGATCTG12360.10751696697767718No Hit
CAATCCCTGCCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCC12080.10508130753158094No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC11840.102993599434927No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACACTCCTGGCGG11670.10151480619979714No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC11610.10099287917563367No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)