FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004976408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2431866
Sequences flagged as poor quality0
Sequence length139-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA1215965.0001110258542205No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTATGGAAGTGATATTCCTTGAG789383.245984770542456No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT712892.93145263760421No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC478041.9657333093188523No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC471891.9404440869686075No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC421871.7347584118532846No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA404851.664771003007567No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTCCCGTGGCTGTCATCAGTG372041.5298540297861807No Hit
CCTCCTCACCACTGATGACAGCCACGGGACCCTTCATTCCAAATTTGGAC351861.4468724839279796No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT324611.3348186125386843No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA296951.221078792992706No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC283331.1650724176414324No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC275451.1326693164837207No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA274111.1271591444594398No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG266491.0958251811571855No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG244321.0046606186360596No Hit
CAAACTCGATGCAGTGCTCCAGGCGCAGCTCAGGCTCCTCCAGGCTCACC230210.9466393296341163No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA219850.90403829816281No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT195170.8025524432678446No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC176490.7257390004218983No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC175490.7216269317470617No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC170810.7023824503488268No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA159550.6560805570701675No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT159130.6543534882267362No Hit
ACCACAGACGCAATCACCACCACAGCATAGACCGAGAGATGTTCCCGACC155140.6379463342141385No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT153210.6300100416717039No Hit
CAGCCAGGGTGGGATCCTTCAGGGCCTTCACAGCCACAAGCATCTTGTCC150510.6189074562496453No Hit
GTTGATGATTTCTAACCTTTTCTGGTTTGCGATGAAAATTTCGGTGATGT146780.6035694400925051No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG146730.6033638366587633No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA143400.5896706479715577No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC142220.5848184069352506No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTATGTGCAGCACATTAAGA140980.5797194417784532No Hit
CAAAAGCAGCAAGTCCAACTGCTATGGATACCCCAAAAGTGTCTTCTTCT138460.5693570287178652No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA136270.5603515983199733No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTGAAACATTGACGGAGAAGTAG135380.5566918571993688No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAAACAAGATAAAGTTATCCGTG132270.5439033236206271No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG129250.5314848762226208No Hit
AGCTGCAGTTGAAAAAGTTCTGCTCCAACTGCAATTCCCGAAGACTCAGC119870.4929136720526542No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT112430.46231988111187045No Hit
CTTTGTCCGTGGTGTTAACACCGGCGGCCTTGAGAACCTTGAGGTAGGGC111990.4605105708949424No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT110710.45524712299115166No Hit
GGCATGTTTGTTGGTGATTCCAAGGAGCTATGACCAGTCCCGAAGATCTG107710.44291091696664214No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG105900.435468072665188No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGGGTCTTTTCAAACATAG105380.433329796954273No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA97380.40043324755558074No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT96430.3965267823144861No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC93730.38542419689242746No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG93640.38505411071169215No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTC93530.38460178315746013No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA91740.3772411802295028No Hit
CAGCATGGAGCCGCTTTTCCATCTTTTCTGTGTTGGTCCAGTATGGTGCT87490.35976488836144754No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA84660.34812773401166014No Hit
GCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGC83550.3435633377825916No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT83410.3429876481681145No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG82870.3407671310837028No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG80400.3306103214568566No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTCGCTCGGCGTGGAGGTTC79870.32843092505919325No Hit
CCGATGTCATTCGCTGCAGTTCCATAATCTTCATAAATTACATCAGGATA78690.32357868402288614No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTTGAATGGCAA78130.3212759255649777No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGTGTCC74780.30750049550427533No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT74760.3074182541307786No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAACTGGAAAGGGCTCCTTGAGG74630.3068836852030498No Hit
CCAAGGACAAGATGCTTGTGGCTGTGAAGGCCCTGAAGGATCCCACCCTG73880.3037996336969224No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG72920.29985204776907937No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGAT70290.28903730715425935No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC67150.27612541151527265No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA61120.2513296374060084No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC60970.25071282710478293No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC59610.24512041370700527No Hit
GCTTCAGCACGATGTCTCTCCTCTTAATGTGCTGCACATACGTGTCCGGC57630.23697851773082892No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGGGTTTGGTTTGGT55270.22727403565821472No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA55150.22678058741723434No Hit
TTGTTATCCTCACCAGCGGGGTGTTGGAGTTCATGGAGGAGCTGGACTCA55030.22628713917625395No Hit
TGGACAGCCTTCAAGACCTGGCCCTGTGCATCCGTAGGTGCAGTTTGGAT54290.22324420835687495No Hit
CTCCATCTCTTTGTCGGTGGTATTAACTCCAGCAGTCTTCAAGATCTGGA54160.22270963942914618No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTGGCAG51730.2127173125492934No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT49480.20346515803091125No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC48570.19972317553681002No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT48370.19890076180184268No Hit
TTCATGGATGCACTGGAGTCAGCAGACACTGTTACCTGTCTGCGCAGAGG48180.19811946875362374No Hit
GTCCGGCTTGGAGGATGGGCCGGTGAGGCGATCGCTCCACAACATCCAGG46650.19182800368112388No Hit
TGAGGTTGGAGATTCGAGAAATGTGATAGTTGGTGCAAAATGCACAGTGA46030.18927852110272522No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC40760.16760791918633675No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG40680.16727895369234982No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT40420.16620981583689234No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG39840.16382481600548715No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT39680.1631668850175133No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG39080.16069964381261137No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC38390.15786231642697418No Hit
TTTTCCGGCCCAGAGCAAGGAAGTGTTATCATTCCAGCCACCTCCACCAC38080.15658757513777485No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG37730.15514835110158207No Hit
GTGGACCAGGAAGGACTCCACTTCCACAGGGGCTCCCCAGGGCTGGGCTT34660.14252430026983395No Hit
CCATGCGACCATCTGCAGCCAGAACCTGTCAGACACATCGAGGAGAGGCA34440.1416196451613699No Hit
GTTAGGTGCGGCCAGATTTGCAGATGGCAAACCACAATTGGGTATCTGCA33380.13726085236604318No Hit
GTGTCCTTACCGTGACGTCCACCGACGAGTACCTGGACCTGTCGGCGCCT33000.1356982662696053No Hit
TCTGCATGGCTTGCAGCTCCTGGTGCTTCCGGCGGTACACAATCATGATG31640.13010585287182763No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGTCGCTTGAGCGGGAAGCGGG30760.1264872324379715No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC30700.1262405083174813No Hit
AGAACAATGTTATGTCGCTTGATGTGCTGAACAAATGTGTCTGGCTTGAG30100.12377326711257938No Hit
GTCCTTCAAGTTTAATATCCCATGATTGTCTATGTTTGAAAAGACCCACG29690.12208731895589642No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTAAGGTGGGAGTTTCATTTT27050.111231457654328No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTGAGTCAGTTTT27030.11114921628083126No Hit
GGCATGTTTGTTGGTGATTCCAAGGAGCTATGACCTGGACATAAAAATAA26910.11065576803985087No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGGCATACGGTTTGG26860.11045016460610906No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT26840.11036792323261231No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC25390.10440542365409936No Hit
GAGTCATCATCATTGCTGATAACGGAGGCTGGGCCTTTCATGCCAAACTT24400.1003344756660112No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)