FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004976412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996722
Sequences flagged as poor quality0
Sequence length139-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA13120713.163851103918645No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT12348512.389111507521656No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT862528.65356639062848No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA522605.24318716753518No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC274512.754128031687873No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG256912.5775492062982455No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC227772.285190855624738No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG209322.1000840755998165No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT204832.055036409349849No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA177831.7841484385816706No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT129861.3028708105168743No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTTGGAGTGCTGCTCTTTTAA111911.1227804743950671No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT110771.1113429822959662No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA109381.0973972682453081No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA97670.9799121520343688No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC66790.6700965765780228No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT64970.6518367207706863No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC53590.5376624575358023No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT48880.49040755596846464No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG48410.48569209869953706No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG47970.48127762806479646No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC47870.48027433928417346No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC44750.4489717293287396No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT42100.4223845766422332No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT40800.40934182249413575No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA37970.3809487500025082No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG36890.37011323117178113No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA36280.3639931696099815No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT31210.3131264284324014No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT30120.302190580723612No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG29760.29857874111336963No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT29300.2939636127225044No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG29180.2927596661857569No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC27740.27831230774478743No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC27230.2731955349636107No Hit
CTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTGCTGAAAATGACTGAATA27200.27289454832942384No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA24770.2485146309602878No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT24640.2472103555454781No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC24490.24570542237454376No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCC24430.24510344910617005No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA22810.22885017086007936No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTG22410.2248370157375878No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC22080.2215261627615323No Hit
GCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGC21980.22052287398090942No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG20510.20577452890575307No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT19940.20005578285620262No Hit
CCAGCAGTCCCGCATCATCATGTACCTGCGGCAGGACTGTAAGGTCAGGG19290.19353440578215392No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC18550.1861100688055446No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTT18410.18470546451267253No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC18080.18139461153661704No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT17610.1766791542676895No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC17400.17457224782838143No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC16120.16173015143640854No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA15820.1587202850945399No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA14820.14868739728831107No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT14810.1485870684102488No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTGCAAGGAAGAGTGGGGTCA14750.14798509514187508No Hit
CAGCCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA13970.14015944265301658No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA13880.139256482750456No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGTGTCC13800.13845385172595767No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC13450.1349423409937776No Hit
GTGTCCTTACCGTGACGTCCACCGACAATGTTATGGAACAGTTCAATCCT12540.12581241309010938No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC11730.11768577396706403No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTC11210.11246867230782505No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAA11140.11176637016138903No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG11080.1111643968930153No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG10810.10845551718533353No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGACTCCTGGCGG10760.10795387279502208No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA10270.10303775776996996No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGGTATG10120.10153282459903563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)