FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004976414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957576
Sequences flagged as poor quality0
Sequence length141-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT11638912.154544391254586No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA10670711.14344971051906No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT827868.645371229019942No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA455314.754818416501667No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC232252.425394955596214No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG219082.287860180288562No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG200152.090173521475058No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC189381.977702030961511No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT164781.7208033618219338No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA155461.6234742725381588No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC123621.2909680276030309No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA122501.2792718280324487No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT104581.0921326349031304No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC98601.0296832836244851No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA96071.0032624042373661No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC92400.9649364645730469No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTTGGAGTGCTGCTCTTTTAA84720.8847339532319106No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT84050.8777371195602229No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT65980.6890314711312732No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC59890.6254333859662314No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT58800.6140504774555754No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC49020.511917591919597No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG48900.5106644276798917No Hit
GCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGC46550.4861232946523305No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC45230.47233848801557266No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT43960.4590758331453587No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT43100.45009482276080437No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT42330.4420536855560289No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA42250.44121824272955884No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC40990.4280600182126536No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA39240.40978470638361864No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG38740.40456318871818003No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC38580.40289230306523977No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC37700.39370243197406785No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA37330.3898385089016433No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG36900.3853480037093661No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG35690.3727119309590048No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG30470.31819928653182616No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA30400.3174682740586648No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT28490.29752207657668944No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT27980.29219612855794214No Hit
CTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTGCTGAAAATGACTGAATA27830.2906296732583106No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT27790.2902119518450755No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGTGTCC27750.2897942304318404No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA26220.2738163863755984No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG25980.2713100578961879No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA25350.26473094563773525No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCC21360.22306323466753553No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT21150.22087019724805132No Hit
GTGTCCTTACCGTGACGTCCACCGACAATGTTATGGAACAGTTCAATCCT20770.21690184382231803No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG19690.2056233656649707No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG19160.20008855693960584No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC17770.1855727378296866No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT17740.1852594467697603No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTC16620.17356324719917793No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTG16600.17335438649256038No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC16290.1701170455399885No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA16190.16907274200690076No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTT15840.16541767964109377No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC15120.15789869420286223No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA14850.1550790746635254No Hit
CCAGCAGTCCCGCATCATCATGTACCTGCGGCAGGACTGTAAGGTCAGGG14800.15455692289698153No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT14620.15267717653742366No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC14570.1521550247708798No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC14230.14860439275838158No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG13390.13983224308044478No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTCTATATTTAGCTCGCTGTT13070.1364904717745641No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC12980.13555059859478516No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT12310.1285537649230975No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTGCAAGGAAGAGTGGGGTCA11680.1219746526646449No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA10750.11226262980692915No Hit
TGGACAGCCTTCAAGACCTGGCCCTGTGCATCCGTAGGTGCAGTTTGGAT10620.11090503521391512No Hit
CAGCCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA10310.10766769426134323No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAA10300.10756326390803446No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT10210.10662339072825551No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGGTATG9900.1033860497756836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)