FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004976424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911066
Sequences flagged as poor quality0
Sequence length141-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA698927.671453001209572No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT688267.554447207995908No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT500325.4915889737955315No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA479265.260431187202683No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC249442.7378916565868994No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC193242.121031846210922No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC191392.1007259627732786No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT188372.067577980080477No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC160731.7641971053688754No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT157621.7300612688872157No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC147081.614372614058696No Hit
GCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGCTGGC146881.6121773834167887No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG132641.4558769617129823No Hit
GCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGGGGCCCCTGTGTCC119181.308137939512615No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA108101.1865221619509454No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG95141.0442712163553465No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA94031.0320876862927604No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC89580.9832438045103208No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC84780.9305582691045434No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA79920.8772141645061938No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT78260.8589937501783625No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG74690.8198088832203156No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC68810.7552691023482382No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC64710.7102668741891366No Hit
GTGTCCTTACCGTGACGTCCACCGACGTAAAGGCGACACAGGAGGAGAAC64490.7078521204830385No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC62490.6858998140639646No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA59560.6537396851600213No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA58750.6448490010602964No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT53480.5870046736460366No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA52080.5716380591526848No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT49690.5454050529818916No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC49130.5392584071845509No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA46880.5145620624630927No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAGCAAGATCGTCAAC44230.4854752564578197No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT42350.46484008842389024No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC40360.4429975435369117No Hit
CAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCCCCGG39330.4316921057310886No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC38390.4213745217141239No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG36580.401507684404862No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT35960.3947024694149491No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGTCGCTTGAGCGGGAAGCGGG35420.38877534668179914No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG35240.38679963910408244No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT35050.38471416999427044No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA33750.37044517082187245No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG33550.368249940179965No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG31830.3493709566595614No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC31480.34552930303622353No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA31100.3413583648165994No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC30200.3314798269280162No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT29540.3242355658097218No Hit
CGCTGCCGAAGACCAACTGCTCCTGCTGGCCGGGCTCTGGGCCCGGGACT29220.32072319678267No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG28950.31775963541609503No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTCTATATTTAGCTCGCTGTT28520.3130398895359941No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC28130.3087591897842747No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT26900.29525852133654423No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC26530.2911973446490156No Hit
CCTGTCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCA25440.27923333765062025No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT25010.27451359177051937No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT23730.2604641156623121No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA23380.2566224620389741No Hit
CTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTGCTGAAAATGACTGAATA21400.23488967868409094No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT21090.2314870711891345No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA20630.22643804071274748No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTC19480.21381546452178No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA19420.21315689532920776No Hit
TGGACAGCCTTCAAGACCTGGCCCTGTGCATCCGTAGGTGCAGTTTGGAT19300.21183975694406332No Hit
TTCATGGATGCACTGGAGTCAGCAGACACTGTTACCTGTCTGCGCAGAGG17430.19131435044222922No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC17330.19021673512127552No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA16220.17803320505868947No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTT14350.15750779855685537No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC14240.1563004217038063No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT13810.15158067582370544No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTTGAATGGCAA13020.1429095147881712No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTTGCTG12180.13368954609216016No Hit
CAGCATGGAGCCGCTTTTCCATCTTTTCTGTGTTGGTCCAGTATGGTGCT12020.13193336157863425No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG12000.13171383851444352No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGAT11540.12666480803805652No Hit
CATGGACGCGTTGGACTCCAGGGACACCTGCTGTCGCTTGAGCGGGAAGC10920.1198595930481436No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG10310.11316413959032605No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC9660.10602964000412704No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTCGCTCGGCGTGGAGGTTC9450.10372464783012428No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC9180.1007610864635493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)