FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004976430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016667
Sequences flagged as poor quality0
Sequence length140-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT11517611.328783170890762No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT798747.856456440506085No Hit
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA747897.356292670067976No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA649786.391276593024068No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG261882.5758680079121286No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC253062.48911393799543No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT251402.472786074496369No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC195011.9181305186457318No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC179441.7649830278744172No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC158451.558524079172433No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA123191.211704520752616No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC108571.067901289212692No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC108281.065048831131531No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA105761.040261954012474No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT89930.8845570870304633No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC88220.8677374204139604No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC81420.8008521964419029No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC72690.7149833721365993No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT71240.7007210817307928No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC70710.6955079686859119No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT69770.6862620700780098No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC66880.6578358498898853No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA61780.607671931910842No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG60450.5945899689868954No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC58050.5709834193496985No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG55110.5420653960441325No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA54130.5324260549422771No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT51930.5107867177748466No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA50980.5014424585434561No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT49860.49042606871276434No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG48060.47272115648486673No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA47440.4666227978285909No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT47340.46563919159370765No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC47100.46327853662998797No Hit
TTCATGGATGCACTGGAGTCAGCAGACACTGTTACCTGTCTGCGCAGAGG46620.4585572267025486No Hit
TGGACAGCCTTCAAGACCTGGCCCAGTGCATCCGTAGGTGCAGTTTGGAT44530.43799985639348976No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA42840.4213769110239636No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA42230.4153769129911761No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTTAATTTGCTTTCTCTTT38940.3830162678635187No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG38490.3785900398065443No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT37610.36993430493957213No Hit
CTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTGCTGAAAATGACTGAATA37370.36757364997585246No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT35550.3496720165009782No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT33590.3303933342972674No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG32450.31918022321959894No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA32380.3184916988551807No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG31670.31150809458750994No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT31250.30737694840100055No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTCTTCCTATGAC30520.3001966228863531No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG27070.26626220778288273No Hit
GTTGATGGTCCTGATCGAGAAACCACAACCTGCATGAAGCGACCCTCTGA26650.2621310615963733No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATAAGGGGTCTGGGCAGTATTC26640.26203270097288495No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTCGCTCGGCGTGGAGGTTC25140.2472786074496369No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC22620.22249173033058023No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTTCTTCCCAGTGATAACCAG21900.21540976543942117No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA21410.2105900948884935No Hit
CTCCATCTCTTTGTCGGTGGTATTAACTCCAGCAGTCTTCAAGATCTGGA20910.20567206371407748No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTTCTGGAAAAGTAGC20110.19780321383501184No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG17590.17301633671595518No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC17060.1678032236710742No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAC16510.1623933893792166No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT16150.15885240693363706No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA16050.15786880069875386No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA15000.14754093523248027No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTT14820.1457704440096905No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTCTATATTTAGCTCGCTGTT14190.1395737247299263No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTGAAACATTGACGGAGAAGTAG13990.1376065122601599No Hit
CCAATGCAGCGAACAATGTTCTGGTGGTTGAATTTGCTGCAGAGCAGAGA13930.13701634851923No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGGGTTTGGTTTGGT13710.13485241480248694No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC13410.13190159609783736No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCACTTACTTGGGCCCT13070.12855733489923446No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACTCCTGGCG12910.12698356492342133No Hit
GCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTGGGGAAGGAGAGGAAA12630.12422946746574837No Hit
AGGATGCCTGCATACACACTGCCCGCCTCGTCAGCCTCCACCAGCTCCTC12570.12363930372481845No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCACTC12380.12177045187854038No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCCGCTGCCACATAAGGTCT11930.11734422382156597No Hit
CTCTGCCTTCTTTGCGCTCGGACATCTCGCCGGAGACGGAGCGCTCTACG11890.11695078132761268No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTGAGTCAGTGCGA11410.11222947140017332No Hit
TGGCCTTCATACACCTCCCCAAAGGCGCCATGGCCCAGACCCCTGTGCAA11110.10927865269552371No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAA10790.10613111274389746No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGTAAGGCTGCCCGTGCCAGAGCC10300.10131144219296977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)