FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004976432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172351
Sequences flagged as poor quality0
Sequence length139-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGA534034.555205736166046No Hit
TCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCG247572.1117395728753587No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAA236432.016716836510567No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCAT207271.7679858677136797No Hit
CGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCC198941.6969320621554467No Hit
CCACGCTGGCCATCACGTAGGCTTCATCGAGGATTTCCTTGTTGGCTTTC158291.3501929029787154No Hit
AGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGC139551.1903431651442273No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGGGAC135291.1540059248467396No Hit
GCGGACCCTGCCATTCCGGAGGAGGTGTGGAATATCAAACAAATGATTAA129021.10052364863424No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCT118921.0143719756284595No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTA116190.9910854343110552No Hit
TTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAACTACC109450.933594119849772No Hit
GGCATGTTTGTTGGTGATTCCAAGGAGCTATGACCTGGACATAAAAATAA108870.9286467960534004No Hit
GTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAAC108170.9226758880232968No Hit
CCAGGACCTCAGCGAGAAAGGAAGTCATCTTCATCCTCAGAAGACAGGAA106760.9106487732769453No Hit
CCAGCAGTCCCGCATCATCATGTACAGCTCGTTGGTGCAGTTACTGGGCT99630.8498308100560327No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCTGGAAAAATGAAGTCAT96980.8272266582277834No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCAGGCAGTCGCTCTCCCGGGGGCC96380.8221087370591231No Hit
GTTAGTGAGCCAGGTAATGAGGCAGGGGGGGTAGCAGACAAACCTGTGGT92240.7867950809953674No Hit
GACAAACTCGTCAGTTTGTTTCGGGTAAAATTGCTGAAAGGAATCAAAAA87270.7444016339816317No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTT79660.6794893338257911No Hit
CACGAGGATGCCAGGTGCAAGCACAGCGGGGGCCTTCATTATGAGAGGTT79200.6755655942631515No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTTGTAGATTGCAGGCAGACA77020.656970480683686No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGTGATG76000.6482700146969637No Hit
TACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCC72000.6141505402392288No Hit
TTGAAGGAGACGGACTGGTGAGAATTTGGGGCCCAATAGAGTCCGAGGCG63300.5399406832936552No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAACTGTGAGGCAAAACA62830.5359316450448713No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCATTCGATTCCTGTCTTCTG61410.5238192316123755No Hit
ATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCAT61240.5223691539479217No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTGAGTCAGTTTT61160.521686764458767No Hit
AATGCATATACATCTGACTGAAAGCTGTATGGATTTTTATCTTGCATTCT60410.5152893629979417No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGCCCCAAATTCTC60320.5145216748226428No Hit
GATCATCCCCTTCCGCACCCGCCTCGGACTCTATTGGGTATGGTTTGACT59260.5054800140913429No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACGGGGACACTTCTTCACG57400.48961445846849616No Hit
ATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTA56100.47852562926973236No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCCACGACATC55660.4747724870793816No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGAGGTATG55350.472128227808907No Hit
CAACAGCACTGTTATTACTACTTGGGTTTTTCCTGTGGCTGAAAAAGAGA54430.464280748683628No Hit
GGTGGTGTTCAAAGAACTTGGAGACAAACAGCAAATCAAGTTGGTCATAA54340.463513060508329No Hit
TGGTCGACGGTCCAAATTTGGAATGAAGGGTAAGGTGGGAGTTTCATTTT53300.45464199714931786No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTGTCCCGAGGCAGCTCCCAGCG52470.44756220619933795No Hit
TCGATGGCACAAGCCATGAAGGGTGTCACCAAGGCCATGGGCACCATGAA51510.43937353232948156No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTTCCAGAACGGTC49810.42487275568494415No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGAGGAGACTCCTG49690.4238491714512121No Hit
CAGAGTCCATTATGATGCTCCAGGTGGCATAACGGACCTTGTAGCCTCCA48810.4163428870705105No Hit
CCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCT46060.3928857483808177No Hit
TGAGGTTGGAGATTCGAGAAATGTGATAGTTGGTGCAACTAAATTGAAAA45860.39117977465793097No Hit
AGTGATGCTTTGTTAATGCGAAGTTCTGACTTCCTATAAGATCAGGACCA45530.3883649180151678No Hit
TGATAGGAGGTGTGGGACTCACTTCGTCAACTGAAAACCAAACACAAAAA44670.3810292310067548No Hit
AGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCA43480.37087868735557866No Hit
CCATGCGACCATCTGCAGCCAGAACCTGTACACATCAAGAGGAATGTGTG43250.36891681757425887No Hit
CATCGTAGCGAACTAATTCACTGCCCAGATCTTAAAACAGAGAGAAAGAA39040.3330060707074929No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTAAAGGAAAGA38950.3322383825321939No Hit
ATCACTCGAGTCCCGTCTACCAAGTGTTTTCTTGATAAAAACAGTAAAAA38050.32456150077920354No Hit
GCACAGCCTCCCTTTCTATAGTAGCTCGCCCTGTGGGGAAGGAGAGGAAA37050.31603163216476976No Hit
CTTGTCCCAGTGGTGGATGTAGATGTAGCTGAAGAGGAGAGAAAGGAGAA36990.3155198400479038No Hit
CAGCCAGGGTGGGATCCTTCAGGGCCTAGGAACAATGCAGGACACAGGTG36980.3154345413617594No Hit
TGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGACTTCGGGATGGCCCG36660.31270498340514064No Hit
GGGGCGAGGTCATCACTGCCGGCCTCTCTTCCAGGGCTGAGTCAGTGCGA36330.3098901267623775No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTCGCTCGGCGTGGAGGTTC36140.3082694517256351No Hit
GCTTCGTCTCGGAATTTGCGGCAGACCTATGGAGGAGAGAGGACACGCTG35560.3033221279292635No Hit
TGTCTTGATTTTACTTTCCCAAATCCAAACCATGAGAAATCTGAAAACCA35010.298630700191325No Hit
TACACGCCAAGTCAATCATCCACAGAGACCTCAAGAGTAATAGTATCCTT34970.2982895054467476No Hit
TGGAGTCAGCTGAGGTGTTGAGAAGGAGAAAGGAGCCTGGAAAGAGACAG34910.29777771332988157No Hit
CATCACCACGAAATCCTTGGTCTCTAATCAAGTCATCAATATTGACAGGT34060.2905273250076129No Hit
ACCACAGACGCAATCACCACCACAGCATAGACCTAGAGAAAGGAAGGGAG33560.28626239070039605No Hit
AAGCACTGTCACCCCTTCCTTGGCACTTTTTTGATTCTTTAATCTTCTAT33160.28285044325462255No Hit
CCACGCTGGCCATCACGTAGGCTTCCTGGAGGGAGGGAGAGGCACGTCAG33150.28276514456847823No Hit
CACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTAAGGGCTGTT32750.27935319712270473No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGACT32730.27918259975041604No Hit
CTCCATCTCTTTGTCGGTGGTATTAACTCCAGCAGTCTTCAAGATCTGGA32240.2750029641293435No Hit
CAGCATGGAGCCGCTTTTCCATCTTTTCTGTGTTGGTCCAGTATGGTGCT31480.2685202639823739No Hit
TTGTTATCCTCACCAGCGGGGTGTTGGAGTTCATGGAGGAGCTGGACTCA31140.2656201086534664No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGACTTGAT30200.2576020321558987No Hit
TCCAACAAAGTCCCATGATATACACAACCAAAATGCCCTGTGCAAAAGAA30090.25666374660831104No Hit
TGCCATTGATGATGCCATGGGTGATGAGGAAGATGAAGACTCCTGGCGGT30000.255896058433012No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTTTGCGGCAA29620.25265470835952714No Hit
GGCAACCCTGCTTGCAGGATGGGCCGGTGAGGGGACCGCTCTGTGGAAGA29210.2491574622276093No Hit
CAGTGTCGGGACTTGAGCTCTCAATATTCCTAATCAAAGGTGCAGTTTCA28550.24352774894208304No Hit
ACCCCAATACATTATCCTGGAACTGATGGAGGGAGGAGACCTTCTTACTT28390.24216296996377365No Hit
CCTTCAAGGGGTGCACTATTTGGGAAAACCTACAAGGACAACATAGAACT27660.23593616587523702No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTGAAACATTGACGGAGAAGTAG27510.23465668558307196No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAG27500.23457138689692764No Hit
CACCAAATTTGTCCAATAGGGCCTCTATATGTTCCTGTGTCAACTTAATC27410.2338036987216286No Hit
TTGAGATGATCAAACTTATAGATATTGCACGACAGACTGCACAGGGCATG27240.23235362105717486No Hit
CTTTGTCCGTGGTGTTAACACCGGCGGCCTAGAAAACAAGGGAAGCAAAA26830.228856374925257No Hit
TGGGAATGCCTGGTTTATTTGGGACTCCAGCTTTAGGGAAAAAAAGAAAA26800.228600478866824No Hit
ACATATGCTCCTTCAGTTGAGGCTGGCATACCAGTGATGATCTCTGAAAA26670.22749159594694762No Hit
CGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCTGCAAGGT24950.21282022193012162No Hit
CCCAATGTGCATATAAACACAATAGAACCTGTCAATATTGATGTAAGTAT24800.21154074163795655No Hit
AGAACAATGTTATGTCGCTTGATGTGCTGAACAACTGCAAAACAAAGACA24770.21128484557952354No Hit
CCCATCCATTTCGGGGTCATTGTTTGAGGCTGCATTGCCGCCTGAGTAGC24700.2106877547765132No Hit
CCAATGCAGCGAACAATGTTCTGGTGGTTGAATTTGCTGCAGAGCAGAGA24510.20906707973977076No Hit
GTGGTGTGCCAAGGTCAAGCTGCTGGCCCTGAAATGAAGAAGGAGGTAGT24460.20864058630904908No Hit
CGGGAATTTTAACTTTCTCACCTTCTGGGATCCAGAGTCCCTTATACACC24390.2080434955060387No Hit
GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGC24180.2062522230970076No Hit
AGGTAGTCTGGGGAAGCTGTAATCTCCTTTTCTCTTCCAGGCGCTAGATT23750.20258437959280112No Hit
GTGAGTCTTTAAATTCACCAATACCTATTCCGTTACACACTGCAGAGATA23730.20241378222051243No Hit
TGTGAAGACTGCGCCAGTTCTCTATGTGTCTGCAGGGGAGGAGGAAAGGT23380.19942832820546066No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTATGGGAAGAAGAAGGGGCACT23230.1981488479132956No Hit
CCCAGTAAGCATCCCTCGAGAAGTAGAGGCCCAATGCCACTGTGGGAAGT22200.18936308324042883No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGGGTTTGGTTTGGT22180.18919248586814016No Hit
AGGAAGATTTTCAATCTCCTCTTGGGTTGGAAGAGTACTGTAAAATAGCA21920.18697472002838741No Hit
GGGCGGGTCTCTCGGAGGAAGGACTTGAGGTCTCCCCCCGCCATGAGCTC21910.18688942134224307No Hit
CATGAACCGTTCTGAGATGAATTAGGAAACTGATCTGTAAGATGAAGGAA21720.18526874630550066No Hit
GTCCGGCTTGGAGGATGGGCCGGTGAGGCGATCGCTCTGGTGGAGAGAGG21430.18279508440731487No Hit
GGGTGCCAGCATTTTAGCATTACTTCATATCTGTTCCAAAAAGAAAGACA21400.18253918834888186No Hit
TGGACAGCCTTCAAGACCTGGCCCTGTGCATCTGTAGGAAGTGAAAGAGA21300.1816862014874385No Hit
CTGGCCCTTGAAGCACTACACAGGCCACTTCCTACAGGAAGCCTCCCTGG21240.18117440937057247No Hit
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTG21090.1798949290784074No Hit
CAAAAGCAGCAAGTCCAACTGCTATGGATACCTGTGAGGAACCAGAAACA21090.1798949290784074No Hit
AGGACGGTTGAAGAAATTGCTTGCTTCCACTAAAAATGACAAATAAAGAA20970.17887134484467537No Hit
GAGTCATCATCATTGCTGATAACGGAGGCTGGGCCTGGAGATGGAAAAAT20700.17656828031877825No Hit
GTTAGGTGCGGCCAGATTTGCAGATGGCAAACCTATGGAACAAGAAAAGT20430.17426521579288115No Hit
CTCAGCGGCTCCCAGGTGCGGGAGAGAGGCCTGCTGAAAATGACTGAATA20200.1723033460115614No Hit
TGGCCTTCATACACCTCCCCAAAGGCGCCATGGCCCAGACCCCTGTGCAA19750.1684649051350662No Hit
GCTCAGGAACCGAGGCGCTGCCGCTGCTGCTGCAGTCACTTGAATGGCAA19170.16351758133869462No Hit
TCAGCACTCTCTTCTGGTACAGCTCCTCCGCCTCTGGAAAGGGATGAGAG19070.16266459447725126No Hit
TGAGGAAATCCAGTTCGTCCTGTTCAGAGCACACTTCAGGCAGCGTCTGG18870.1609586207543645No Hit
CAGGACCTTGGCTGCATGAAGTTTTAACATGGTAAAACTCATTGTATTTC18780.16019093257906547No Hit
CCCAAGCCATTCAATGGGATCTTCGTGATCTGTGAAGAGAAGGGGTGGAA18510.1578878680531684No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCTA18490.1577172706808797No Hit
CTTTGTCCGTGGTGTTAACACCGGCGGCCTTGAGAACCTTGAGGTAGGGC18410.157034881191725No Hit
GTGAGCTCGATCCTCCTTTTCAAACTGACCCTGAGGAAAGGAAAAAAACC18210.15532890746883826No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCGCAC18130.15464651797968354No Hit
CCGATGTCATTCGCTGCAGTTCCATAATCTTAAGTGGGCAAAGGGGGAAT18080.1542200245489619No Hit
TGCGGGCAAGAGTCCAGAGGACCTTGAGAGGCTGCTGCCACATAAGGTCA17870.1524287521399308No Hit
TTCATGGATGCACTGGAGTCAGCAGACACTGTTACCTGTCTGCGCAGAGG17630.15038158367246668No Hit
CGCACGAGCCGTGATCTGTCACCACATAATTACCTGGGGAAGAGAGCATG17250.14714023359898187No Hit
ATTGTGTTCACCAAGCTCACACCATTCATGCTTGGAGCCTTGGTCGGTGA16910.1442400782700744No Hit
TGGACAGCCTTCAAGACCTGGCCCTGTGCATCCGTAGGTGCAGTTTGGAT16700.1424488058610433No Hit
GTTGATGATTTCTAACCTTTTCTGGTTTGCGATGAAACTGTAACAAAGCA16500.14074283213815658No Hit
TTCTCCTGGTCTGTGGAACCCTTCTTCAAAGTCTATACAACATAAAAACA16370.13963394921828018No Hit
GTCCTTCAAGTTTAATATCCCATGATTGTCTATGTTTGAAAAGACCCCTG16260.13869566367069247No Hit
ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTC15700.13391893724660958No Hit
GCTTCAGCACGATGTCTCTCCTCTTAATGTGCTGCACATCTGTAGGATGG15490.1321276648375785No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGGCATACGGTTTGG14950.1275215357857843No Hit
TTCATGGATGCACTGGAGTCAGCAGACACCTGCAAGGAAGAGTGGGGTCA14590.12445078308458814No Hit
CTGAGGTGTAGGTGCTGTCACATTCAACATTTTCACTGCCACATCACCAT14550.1241095883400108No Hit
AAATTGGGAAACTTCTCCTATGTCAGTGATTCCTGAGAAATCCAGTGGTG13970.11916226454363923No Hit
CGAGTCGGGCTCTGGAGGAAAAGAAAGGTAAGGGCGTGTCTCGCCGGCTC13870.11830927768219587No Hit
CATCATCCTCCGAGGAGGGGAGAGCATCTTGTTCAGGCAAGGTCGGACTC13590.11592091447015442No Hit
GTGTTCCCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGA13560.1156650184117214No Hit
TCTCCCAGGGGTTTGCCTAAGACCAGTCTTTCGGGGGAAACAGAGAGTGG13510.11523852498099972No Hit
GTGGACCAGGAAGGACTCCACTTCCACAGGGGCTCCCCAGGGCTGGGCTG13480.11498262892256671No Hit
CCAGCAGTCCCGCATCATCATGTACCTGCGGCAGGACTGTAAGGTCAGGG13480.11498262892256671No Hit
CAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGACTC12750.10875582483403008No Hit
GGGACAAGCCGAAATCCGAAATCTTCATCTTCCGCCCCTCAGCTACCAGG12740.10867052614788575No Hit
TCAGGGACACAACTGCCACAAGCCGGACACGTGTAAGTACTGGGATGGAA12460.1062821629358443No Hit
GGTTGTAGTCGGTCATGATGGTCGAGGTGCGGAGCTTGCTCAGCTTGTAC12440.10611156556355564No Hit
CTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAACCTGCACTC12330.1051732800159679No Hit
TTCCGAGGGAATTCCCACTTTGGATCCTCCTATGTTGGAAATGAGGGAAG12060.1028702154900708No Hit
TCCATCTTTTCTGGGGATGTCCAATATGGAGCTACGGCTGCCCGGGGAAA11940.10184663125633875No Hit
CCTCCTCACCACTGATGACAGCCACGGGACCTGCACACACCAAGAGAGAC11920.10167603388405008No Hit
TTTTCCGGCCCAGAGCAAGGAAGTGTTATCATTCCAGCCACCTCCACCAC11900.10150543651176142No Hit
CTCCATCTCTTTGTCGGTGGTATTAACTCCAGCAGTCTAGAAGAGACAAC11750.10022595621959635No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)