FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004976447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25104774
Sequences flagged as poor quality0
Sequence length35-231
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA7595183.0253927002091316No Hit
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG7588563.022755751555461No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT5392902.1481571592717783No Hit
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT4961321.97624563359941No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG4518981.8000480705382966No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA4509141.7961284973129015No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG4507791.7955907509862468No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT4093831.6306978107032548No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG4034731.6071564715141433No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC3763801.4992367587137012No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT3591491.4306004109019266No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT3459711.3781084028081672No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA3381761.347058531576504No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT3211381.2791909618465396No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG3080971.2272446666916819No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT3057831.2180272963222054No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG3057171.2177643981180633No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG3030701.2072205868095047No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA2926091.1655512214529395No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT2877451.1461764204688718No Hit
GTGATTGAAGCTGAGTACATTTTGCTGGTGTATTTTTAGGTAAAATGCTT2862991.1404165598144798No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA2811971.1200937319730502No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG2423550.9653741555291436No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC2296550.9147861677623546No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG2130740.8487389689307699No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG2103340.8378247101527382No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT2071530.8251538133743009No Hit
GATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGTATTATCAAGTAAGA2068480.8239389050066732No Hit
CCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAGTGTTGGGGCAATC1987620.7917298916931098No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT1966840.7834525815687486No Hit
GCATGATGTGCTGTTAGAATCAGATGTTACTGCTAAAATTTACATGTTGT1853270.7382141739256446No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT1836980.7317253682506761No Hit
GGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAA1791500.713609292001593No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA1682980.6703824539507904No Hit
ATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATATGAGTGCTTGGCT1663340.6625592407244932No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA1649170.656914895947679No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC1523080.6066893890381169No Hit
CCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAGGAGCACCACCCCAG1438140.5728551868262188No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG1374340.5474416937591232No Hit
GAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAG1317450.5247806652232759No Hit
TTTAGGTAAAATGCTTTTTGTTCATTTCTGGTGGTGGGAGGGGACTGAAG1274120.5075209997907171No Hit
CATCTGGAGGCAAACCCTGACACTGGAGTGCTCACAGTCTCCTGGGAGAG1165490.46425034537255744No Hit
AGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT1118540.44554872312333904No Hit
CTCTGAAGGCTCTATGAAAGGAATAGCATGATGTGCTGTTAGAATCAGAT996900.39709578743867596No Hit
GAAGCCGAAATAAATGAGAGATGAGTTGGGATCAAGTGGATTGAGGAGGC840880.3349482453018697No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGT733900.29233483639406593No Hit
AGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACG713180.28408142610644493No Hit
TCAAGAGTAATTACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGA697240.2777320361457944No Hit
CACTGCTCGACCTGAGAGACCCATGGCATTCCTCAGGGAATACTTTGAGA684630.27270908712422587No Hit
GAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGG635540.25315503736460643No Hit
CGGTCAGAAAATGAAGAGTTTGTTGAAGTGGGAAGATTGGGGCCTTCTGA609060.2426072427499248No Hit
CGCAAGAAAGTGGAGGCGCAGCTGCAGGAGCTGCAGGTCAAGTTCAACGA578170.23030281013483733No Hit
AGAAGGTGACCACCGAGGCGAAGCTGAAAAAGCTGGAGGAGGAGCAGATC554140.2207309255203811No Hit
GGGAACCACTAGTTCTTTCAGATGGTATTCTTCAGACTATAGAAGGAGCT536170.21357292441668663No Hit
ATGGCAGAGCTCACGTGCCTCAACGAAGCCTCGGTGCTGCACAACCTCAA530050.21113514106918468No Hit
AGGAGGAGGAGGCCAAGCACAACCTGGAGAAGCAGATCGCCACCCTCCAT529020.21072486053847767No Hit
CGGAAGAAGCTGGAGATGGACCTGAAGGACCTGGAGGCGCACATCGACTC522340.20806401204806704No Hit
CACCGAGAGCAGCCACTGTCAAAGCAAAGACAAATGTGAAATTGTGGGGC520230.2072235344560361No Hit
GCAGAGACCCGTGCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAA489400.19494300167768888No Hit
ATTCTTCAGACTATAGAAGGAGCTTCCAGTTGAATTCACCAGTGGACAAA453250.1805433500417092No Hit
GCGCGAGAAGGAGGAGTTGATGCTGCGGCTGCAGGACTATGAGGAGAAGA441280.17577533261203626No Hit
TGAAGGTCAGAGAGAAGCAGCTGGCTGCGGAGAACAGGCTCACGGAGATG415250.1654067867729062No Hit
GAAGTGATATTTTTGATGCCATGTTTTCGGTCTCCTTTATCGCAGGAGAG407460.16230379130280162No Hit
GCTGCTCAAAGATTCTATTGTGCAGTTGTGCACTGCTCGACCTGAGAGAC398980.15892594771018453No Hit
GAGATCTGCCATGACCTAGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTG386750.15405436432130398No Hit
GCACAATATCATGGATTTTGCCATGCCCTATTTCATCCAGGTCATGAAGG374310.1490991315038327No Hit
CGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCCGACAACA353280.14072223872638726No Hit
CACTGTCAAAGCAAAGACAAATGTGAAATTGTGGGGCATCGACCGAGACA346760.13812512313395053No Hit
GCACGAGATGCCCCCTCACATCTATGCCATCACAGACACCGCCTACAGGA341190.1359064216232339No Hit
CGTCTGGAGGCCCGCATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCA340040.13544834141904644No Hit
CAGGGCAACACGGAGCTGATCAACGACCGGCTGAAGAAGGCCAACCTGCA336290.133954601622783No Hit
ACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGATTATTTTTTAAA323520.1288679197032405No Hit
GCACATCGACTCGGCCAACAAGAACCGGGACGAAGCCATCAAACAGCTGC322890.12861697141746825No Hit
GAGGTCAACCTGCAGGCCATGAAGGCCCAGTTCGAGCGGGACCTGCAGGG318130.12672091770274452No Hit
CCCAAGACCGTGCGCGACACGCTGCTGGCGCTGCACCAGCACGGCCACTC316650.12613138839648586No Hit
CTCGTGAGGAGATCCTGGCCCAGGCCAAAGAGAACGAGAAGAAGCTGAAG309200.1231638253345758No Hit
GCTCTGCTGAACTCCAAGGAGGCCGCACTGAGCACTGCTCTCAGTGAGAA306980.12227953137518785No Hit
CCCAGACTACGACTTGTGTAGCGTCTGCGAGGGAAAGGGCTTGCACCGGG305570.12171788521179279No Hit
GAGGAGGCCAAGACCCACGAGGCCCAGATCCAGGAGATGAGGCAGAAGCA301320.12002498010936088No Hit
CATCGAGTGGAACTTCATCGACTTTGGCCTCGACCTGCAGCCCTGCATCG297950.11868260594578545No Hit
GGCTGCGGAGAACAGGCTCACGGAGATGGAGACGCTGCAGTCTCAGCTCA290990.11591022488392048No Hit
CCCACGTCCTCCTCGTGCAGGGGAGGCCCGCCCTGGCCCCACGGCAGAAT290090.11555172733281727No Hit
GTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTACAAGGA289130.11516932994497381No Hit
ATCTAAGAGCCTCGCCAAGCTCAAGAACAAGCATGAGGCAATGATCACTG287300.11444038492439725No Hit
CTGTGCTACAACGTCGGTCAGAAAATGAAGAGTTTGTTGAAGTGGGAAGA286790.11423723631210543No Hit
GCTGGACGACCTGCTGGTGGACCTGGACCACCAGCGCCAGAGCGCGTGCA285770.11383093908752175No Hit
GAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAGGAGCTCCAGGCCGCCCT283400.11288689553628325No Hit
AAAGTCCTCTCGGGAGAAGCAGCGCCTGTTCCCGGGGCAGATCCAGGTTC277290.11045309549490467No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTTTGTCTGTTCTG275660.10980381659679549No Hit
TGAAAAAGCTGGAGGAGGAGCAGATCATCCTGGAGGACCAGAACTGCAAG273090.10878010692308962No Hit
CAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGATTATGGGCATCC271100.10798742900453913No Hit
CCCCGAGCCTGAGGCGGAAGCCGAGGCTGCGGCGGGTCCGGGACCCTGCG269140.10720670100435878No Hit
AAAACAGAGTTGGAGGACACGCTGGATTCCACAGCTGCCCAGCAGGAGCT267300.10647377267765884No Hit
GCTGCACTGCGGGCTAAGGAGGAGCTGGAGAGACAGGCGGTGGATCAGAT260570.10379300765663137No Hit
CTGAATGACAACATCGCCACACTGCTCCACCAGTCCTCTGACAAGTTTGT253190.10085332773758489No Hit
CAGAATCCGAAGGGCCAAGCTCTCTGGACCCCTCCCAGGAGGGACCCACA251590.10021599875784581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)