FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16435628
Sequences flagged as poor quality0
Sequence length35-231
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA9174365.5819954065643245No Hit
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG7050454.289735688834038No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC2864151.742647132193549No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA2672121.6258094914292291No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA2446901.4887779158788457No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT2442931.4863624316637003No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG2384311.4506960123458623No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG2316181.4092433827292756No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG2183781.3286866799370247No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT2058051.2521882339999422No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT1703321.0363583308164435No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT1694521.0310041088785897No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC1577960.9600850055744752No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG1524250.927405998724235No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG1367730.8321738603477761No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG1332090.8104892614994693No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA1095550.6665702095472105No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT1066010.6485970599967339No Hit
GTGATTGAAGCTGAGTACATTTTGCTGGTGTATTTTTAGGTAAAATGCTT1049770.6387160867841497No Hit
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT1042870.6345178900374236No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG1020950.6211810099376793No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT999990.6084282267766099No Hit
GGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAA869560.5290701395772648No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC748190.4552244672366642No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT736630.4481909666001202No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA729140.44363379360983346No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT702910.4276745616291632No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT699550.42563022234380093No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA671520.4085758086031151No Hit
CGCAAGAAAGTGGAGGCGCAGCTGCAGGAGCTGCAGGTCAAGTTCAACGA671460.4085393025444479No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA655610.39889561871320034No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG585740.35638431339526544No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG582470.354394733197904No Hit
CCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAGTGTTGGGGCAATC567860.34550550791244483No Hit
GCAGAGACCCGTGCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAA528120.32132632838854713No Hit
GATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGTATTATCAAGTAAGA522460.3178825901876095No Hit
GCGCGAGAAGGAGGAGTTGATGCTGCGGCTGCAGGACTATGAGGAGAAGA459920.27983110837018216No Hit
ATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATATGAGTGCTTGGCT424190.2580917504338745No Hit
GCACGAGATGCCCCCTCACATCTATGCCATCACAGACACCGCCTACAGGA416450.25338246886580784No Hit
AGGAGGAGGAGGCCAAGCACAACCTGGAGAAGCAGATCGCCACCCTCCAT408100.24830204236795822No Hit
ATGGCAGAGCTCACGTGCCTCAACGAAGCCTCGGTGCTGCACAACCTCAA386370.23508076478732667No Hit
CGTCTGGAGGCCCGCATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCA379030.23061485694370792No Hit
GCATGATGTGCTGTTAGAATCAGATGTTACTGCTAAAATTTACATGTTGT337940.20561429109979856No Hit
TGAAGGTCAGAGAGAAGCAGCTGGCTGCGGAGAACAGGCTCACGGAGATG337180.20515188102334758No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT313750.19089626511381252No Hit
AGAAGGTGACCACCGAGGCGAAGCTGAAAAAGCTGGAGGAGGAGCAGATC313450.1907137348204766No Hit
CCCAGACTACGACTTGTGTAGCGTCTGCGAGGGAAAGGGCTTGCACCGGG300390.18276758271725302No Hit
TTTAGGTAAAATGCTTTTTGTTCATTTCTGGTGGTGGGAGGGGACTGAAG299140.18200703982835337No Hit
CGGAAGAAGCTGGAGATGGACCTGAAGGACCTGGAGGCGCACATCGACTC296920.1806563156576676No Hit
CAGGGCAACACGGAGCTGATCAACGACCGGCTGAAGAAGGCCAACCTGCA292520.17797920468874084No Hit
GCTGGACGACCTGCTGGTGGACCTGGACCACCAGCGCCAGAGCGCGTGCA291340.17726125220161953No Hit
CGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCCGACAACA278200.16926642535350642No Hit
GAGATCTGCCATGACCTAGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTG277420.16879184659083304No Hit
GGCTGCGGAGAACAGGCTCACGGAGATGGAGACGCTGCAGTCTCAGCTCA273950.1666805795312476No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG272190.1656097351436769No Hit
CTCGTGAGGAGATCCTGGCCCAGGCCAAAGAGAACGAGAAGAAGCTGAAG271110.16495262608766761No Hit
CAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGATTATGGGCATCC270000.1642772640023247No Hit
CGGGCAGCAGGACAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGA255070.15519333973730728No Hit
GCTCTGCTGAACTCCAAGGAGGCCGCACTGAGCACTGCTCTCAGTGAGAA250490.15240671059237895No Hit
CCCAGGCCAAAGAGAACGAGAAGAAGCTGAAGAGCATGGAGGCCGAGATG250120.15218158989726466No Hit
CGCACGGAGATGGAGGACCTTATGAGCTCCAAGGATGATGTGGGCAAGAG246090.14972959962345217No Hit
AGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACG244900.1490055627932197No Hit
CCAGTTCGAGCGGGACCTGCAGGGCCGGGACGAGCAGAGCGAGGAGAAGA241660.1470342356251918No Hit
CCCTCGAGGCCAAGATTGCACAGCTGGAGGAGCAGCTGGACAACGAGACC239060.14545230641628054No Hit
GAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAG238770.1452758604660558No Hit
GACCCGTGCTGAATTTGCAGAGAGAACGGTTGCAAAACTGGAAAAGACAA220600.13422060903301047No Hit
GAGGAGGCCAAGACCCACGAGGCCCAGATCCAGGAGATGAGGCAGAAGCA220480.1341475969156761No Hit
GAGGTCAACCTGCAGGCCATGAAGGCCCAGTTCGAGCGGGACCTGCAGGG219610.13361825906500197No Hit
GAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAGGAGCTCCAGGCCGCCCT215040.13083771426318483No Hit
GCTGCACTGCGGGCTAAGGAGGAGCTGGAGAGACAGGCGGTGGATCAGAT211240.1285256638809299No Hit
CCCCAAGTTCCAGAAGCCCAAGCAGCTGAAGGACAAAGCTGATTTCTGCA210680.12818494066670286No Hit
TCCAGGCCCAGATCGCGGAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAG210580.12812409723559087No Hit
AGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT210400.12801457905958932No Hit
GGCACACCAAGCTCGCATTCCCCAGCCCCTTCGGGCACCTGTCTGAGGGC208700.12698024073068578No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGT206720.12577554079466874No Hit
CCAAGCAGCTGAAGGACAAAGCTGATTTCTGCATTATCCACTATGCCGGC204140.12420578027197988No Hit
GAAGAAGCTGAAGGATGTGCTGCTGCAGGTGGATGACGAGCGGAGGAACG201330.12249607985773345No Hit
TGAAAAAGCTGGAGGAGGAGCAGATCATCCTGGAGGACCAGAACTGCAAG196990.11985547494747387No Hit
GTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTACAAGGA193470.11771378617233244No Hit
CATCGAGTGGAACTTCATCGACTTTGGCCTCGACCTGCAGCCCTGCATCG192370.11704450843010075No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGGGAAGTGAAACTGT191630.11659426703987216No Hit
CACTGCTCCACCAGTCCTCTGACAAGTTTGTCTCGGAGCTGTGGAAGGAT189550.11532872367274315No Hit
CTGAATGACAACATCGCCACACTGCTCCACCAGTCCTCTGACAAGTTTGT185350.1127732995660403No Hit
CCTCGGTGCTGCACAACCTCAAGGAGCGTTACTACTCAGGGCTCATCTAC183560.11168420214913602No Hit
CAGCTCGGCAACATCGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTC177620.10807010234108487No Hit
CCCACGTCCTCCTCGTGCAGGGGAGGCCCGCCCTGGCCCCACGGCAGAAT177400.10793624679263852No Hit
GCCTCCATCACCGCCCTCGAGGCCAAGATTGCACAGCTGGAGGAGCAGCT177050.10772329478374663No Hit
CGGAAGAAGCTGGAGATGGACCTGAAGGACCTGGAGGCTCACGTCGACTC172410.10490015958015111No Hit
GCTGGAGAGACAGGCGGTGGATCAGATAAAGAGCCAGGAGCAGCTGGCTG172200.10477238837481596No Hit
GCACATCGACTCGGCCAACAAGAACCGGGACGAAGCCATCAAACAGCTGC171740.10449250859170091No Hit
GCAAGCCGGGTGGGAATGTTGAGGGCGCCACGCAGTCTCTGGCGGAGCAG171420.10429780961214259No Hit
CTCTGAAGGCTCTATGAAAGGAATAGCATGATGTGCTGTTAGAATCAGAT170250.10358594146813252No Hit
AGTTGCAGGAGGAACTGGCAGCCGCGGAGCGTGCCAAGCGCCAGGCCCAG170120.10350684500768695No Hit
GCCCGGGCAGGAATCTGATGACTTTGAGCTGTCTGGCTCTGGAGATCTGG169690.10324521825390548No Hit
ATCTAAGAGCCTCGCCAAGCTCAAGAACAAGCATGAGGCAATGATCACTG169370.10305051927434718No Hit
GCTGGAGGACGCCACTGAGACGGCCGATGCCATGAACCGCGAAGTCAGCT168560.10255768748234019No Hit
CCGCACTGAGCACTGCTCTCAGTGAGAAGCGCACGCTGGAGGGCGAGCTG166950.10157810824143745No Hit
CTGCAGGTCAAGTTCAACGAGGGAGAGCGCGTGCGCACAGAGCTGGCCGA166490.10129822845832237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACAT35150.0185.80699225
GGTGCTT14250.0182.15437225
CCAGTAA52100.0152.17815210-214
CTAATAT79250.0135.34395175-179
TTCTAAT77700.0135.16011175-179
TAATGGG77450.0128.01715180-184
GGGGAGT53600.0116.07561185-189
TTTTCTA87050.0115.85654170-174
TAATATA96800.0112.41614175-179
ATAATGG90100.0111.868164180-184
TATTTAT83050.0109.73875165-169
ACCTTTT72600.0109.54415160-164
CCTTTTA76450.0107.31261160-164
TGATAGT11800.0106.43931225
AGTAAGT15250.0106.13328210-214
GTAACCT74800.0105.18259155-159
CTTTTAT78600.0105.055160-164
TATAATG96650.0104.71204180-184
ATTTTCT97100.0103.1173170-174
GGTAACC82250.0100.165155-159
Produced by FastQC (version 0.11.5)