FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004976461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30432410
Sequences flagged as poor quality0
Sequence length35-231
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG8579762.81928378330865No Hit
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA7152752.35037251404013No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA5472141.7981290341448475No Hit
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT5244001.7231629042852667No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT4813841.5818135993830262No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG4494301.4768136996051249No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG4091711.344523815235139No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG3953001.2989441191151145No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG3469421.140041160065864No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT3462731.1378428458344245No Hit
CCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAGTGTTGGGGCAATC3461621.1374781031144099No Hit
GTGATTGAAGCTGAGTACATTTTGCTGGTGTATTTTTAGGTAAAATGCTT3348311.100244771938864No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC3298771.08396607432668No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG3220601.0582796433144794No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT3128851.0281308644303886No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA3067301.0079057163070555No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT3057191.004583600181517No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA2910710.9564507050213901No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC2905800.9548372935301542No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT2888040.9490014100099203No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT2762040.9075981823325856No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT2718000.8931267684682218No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG2628290.8636483275560497No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT2591080.8514212315094335No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA2521380.8285180174688761No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG2463970.8096532611120841No Hit
GGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAA2449200.8047998827565744No Hit
GATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGTATTATCAAGTAAGA2426980.7974984564153808No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG2382470.7828726019398398No Hit
AGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT2354460.7736685987077593No Hit
TTTAGGTAAAATGCTTTTTGTTCATTTCTGGTGGTGGGAGGGGACTGAAG2139580.7030596656656505No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC2127460.699077069479545No Hit
GCATGATGTGCTGTTAGAATCAGATGTTACTGCTAAAATTTACATGTTGT2102160.6907635642395722No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT2083700.6846976627877976No Hit
GAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAG1965260.6457786287711029No Hit
TCAAGAGTAATTACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGA1946450.6395977183535579No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA1901230.6247385599760256No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG1891420.6215150229640045No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT1849490.6077369488647136No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA1816920.5970345431071676No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG1748550.5745683631365376No Hit
ATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATATGAGTGCTTGGCT1747010.574062323687148No Hit
AGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACG1210950.39791459171324256No Hit
GAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGG1175930.38640712319530396No Hit
CGCAAGAAAGTGGAGGCGCAGCTGCAGGAGCTGCAGGTCAAGTTCAACGA1156520.38002905455072405No Hit
CGGAAGAAGCTGGAGATGGACCTGAAGGACCTGGAGGCGCACATCGACTC1130730.3715545367586727No Hit
ACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGATTATTTTTTAAA1111060.3650910328823777No Hit
AGGAGGAGGAGGCCAAGCACAACCTGGAGAAGCAGATCGCCACCCTCCAT1103710.36267584460119984No Hit
ATGGCAGAGCTCACGTGCCTCAACGAAGCCTCGGTGCTGCACAACCTCAA1082540.35571944515731746No Hit
AGAAGGTGACCACCGAGGCGAAGCTGAAAAAGCTGGAGGAGGAGCAGATC1070830.3518715737596858No Hit
GGGAACCACTAGTTCTTTCAGATGGTATTCTTCAGACTATAGAAGGAGCT1017550.3343639231989842No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGT995270.32704278103508727No Hit
CTCTGAAGGCTCTATGAAAGGAATAGCATGATGTGCTGTTAGAATCAGAT929020.30527322679998065No Hit
TGAAGGTCAGAGAGAAGCAGCTGGCTGCGGAGAACAGGCTCACGGAGATG838720.2756009136312241No Hit
GAAGCCGAAATAAATGAGAGATGAGTTGGGATCAAGTGGATTGAGGAGGC789140.2593090721372379No Hit
CGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCCGACAACA782160.25701546476273157No Hit
CAGGGCAACACGGAGCTGATCAACGACCGGCTGAAGAAGGCCAACCTGCA709580.23316589123240652No Hit
GAGATCTGCCATGACCTAGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTG696140.22874954694682412No Hit
ATTCTTCAGACTATAGAAGGAGCTTCCAGTTGAATTCACCAGTGGACAAA692170.227445016677943No Hit
CGTCTGGAGGCCCGCATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCA673800.22140868896022367No Hit
GCACATCGACTCGGCCAACAAGAACCGGGACGAAGCCATCAAACAGCTGC671670.22070877725424964No Hit
TGAAAAAGCTGGAGGAGGAGCAGATCATCCTGGAGGACCAGAACTGCAAG646860.2125562845663554No Hit
GAGGTCAACCTGCAGGCCATGAAGGCCCAGTTCGAGCGGGACCTGCAGGG630550.20719686676145596No Hit
ATCTAAGAGCCTCGCCAAGCTCAAGAACAAGCATGAGGCAATGATCACTG596530.19601799528857558No Hit
CTGAAGAGATTGTGGAAATGTACAAGGGCAAGAAGAGGCACGAGATGCCC591160.19425342915661295No Hit
GAAATTAAACTTCTGTCTGACAAACTGAAAGAGGCTGAGACCCGTGCTGA585750.19247571914284803No Hit
CTCGTGAGGAGATCCTGGCCCAGGCCAAAGAGAACGAGAAGAAGCTGAAG579850.19053699657700457No Hit
GCTGGACGACCTGCTGGTGGACCTGGACCACCAGCGCCAGAGCGCGTGCA575590.18913717316505657No Hit
GTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTACAAGGA572190.18801994321185866No Hit
GCAACAGGTGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC570020.18730688762408235No Hit
GGCTGCGGAGAACAGGCTCACGGAGATGGAGACGCTGCAGTCTCAGCTCA557300.1831271332109419No Hit
CATCGAGTGGAACTTCATCGACTTTGGCCTCGACCTGCAGCCCTGCATCG555770.18262437973200282No Hit
CAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGATTATGGGCATCC554190.18210519640081085No Hit
CGCACGGAGATGGAGGACCTTATGAGCTCCAAGGATGATGTGGGCAAGAG549980.18072180284111578No Hit
GAGGAGGCCAAGACCCACGAGGCCCAGATCCAGGAGATGAGGCAGAAGCA546080.17944027436538876No Hit
AAAACAGAGTTGGAGGACACGCTGGATTCCACAGCTGCCCAGCAGGAGCT541490.17793201392857153No Hit
CCAAGCAGCTGAAGGACAAAGCTGATTTCTGCATTATCCACTATGCCGGC525430.17265474538493664No Hit
TCCAGGCCCAGATCGCGGAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAG523550.1720369829402272No Hit
GCACGAGATGCCCCCTCACATCTATGCCATCACAGACACCGCCTACAGGA518030.17022312725150587No Hit
GAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAGGAGCTCCAGGCCGCCCT500210.16436752790856854No Hit
CTGAATGACAACATCGCCACACTGCTCCACCAGTCCTCTGACAAGTTTGT497890.1636051827640335No Hit
CCCCAAGTTCCAGAAGCCCAAGCAGCTGAAGGACAAAGCTGATTTCTGCA491970.16165988825728886No Hit
AGTTGCAGGAGGAACTGGCAGCCGCGGAGCGTGCCAAGCGCCAGGCCCAG472880.1553869706671276No Hit
GACCCGTGCTGAATTTGCAGAGAGAACGGTTGCAAAACTGGAAAAGACAA466460.15327737763785385No Hit
GCAGAGACCCGTGCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAA459530.15100020011560045No Hit
AAAGTCCTCTCGGGAGAAGCAGCGCCTGTTCCCGGGGCAGATCCAGGTTC457840.15044487110945207No Hit
CCCAGGCCAAAGAGAACGAGAAGAAGCTGAAGAGCATGGAGGCCGAGATG444320.14600223906026502No Hit
CACTGCTCCACCAGTCCTCTGACAAGTTTGTCTCGGAGCTGTGGAAGGAT441040.1449244407524741No Hit
GAAGAAGCTGAAGGATGTGCTGCTGCAGGTGGATGACGAGCGGAGGAACG438570.14411280605118032No Hit
CTGCAGGTCAAGTTCAACGAGGGAGAGCGCGTGCGCACAGAGCTGGCCGA436460.14341946628610747No Hit
CCCAGACTACGACTTGTGTAGCGTCTGCGAGGGAAAGGGCTTGCACCGGG435230.14301529192068588No Hit
CCCTCGAGGCCAAGATTGCACAGCTGGAGGAGCAGCTGGACAACGAGACC427410.14044566302833067No Hit
CTGCTGCAGGCCAACCCCATCCTGGAGGCCTTCGGGAACGCCAAGACCGT427010.14031422421030737No Hit
GACGTCATCATAGGGTTCCAGGCCTGCTGCAGGGGCTACCTGGCCAGGAA423820.13926599963657166No Hit
ATGGAGGCCGAGATGATCCAGTTGCAGGAGGAACTGGCAGCCGCGGAGCG418960.13766901799758877No Hit
CAGCTCGGCAACATCGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTC418430.1374948615637079No Hit
CAGAATCCGAAGGGCCAAGCTCTCTGGACCCCTCCCAGGAGGGACCCACA415610.13656821789664375No Hit
CCAGTATCTGGCGTACGTGGCGTCCTCGCACAAGAGCAAGAAGGACCAGG409520.13456706189223921No Hit
GCCAGGGCTTCCCCAACAGGGTGGTCTTCCAGGAGTTTCGGCAGAGATAT405210.13315080862803833No Hit
CTGAGGAACACGAACCCCAACTTTGTCCGCTGCATCATCCCCAACCACGA393670.12935879872806655No Hit
GGAATTGACAATGGCCATGTCCTACGTGAAGGATGACATCTTCCGAATCT391850.1287607521060606No Hit
CCAGTTCGAGCGGGACCTGCAGGGCCGGGACGAGCAGAGCGAGGAGAAGA391500.12864574314029023No Hit
TTTGGAGAAATCTCGTGCTATCCGCCAAGCCAAGGAAGAACGGACCTTCC383980.12617469336145246No Hit
GCTGGAGGACGCCACTGAGACGGCCGATGCCATGAACCGCGAAGTCAGCT382560.12570808555746982No Hit
GCCTCCATCACCGCCCTCGAGGCCAAGATTGCACAGCTGGAGGAGCAGCT377020.12388765792784731No Hit
CAACAAGCAGTTCCGCACGGAGATGGAGGACCTTATGAGCTCCAAGGATG376280.12364449611450425No Hit
ACAGAGGAGAAGAGCAGCTCACAGCCAAGCAGCTGCTGCTCTGACCCCAG374910.12319431816277449No Hit
CCCACGTCCTCCTCGTGCAGGGGAGGCCCGCCCTGGCCCCACGGCAGAAT364370.11973090530786093No Hit
CCTCGGTGCTGCACAACCTCAAGGAGCGTTACTACTCAGGGCTCATCTAC355090.11668152472972071No Hit
GCTCTGCTGAACTCCAAGGAGGCCGCACTGAGCACTGCTCTCAGTGAGAA353980.11631678200970609No Hit
TAGAAAGCTGTCTCCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAG343250.11279093571623147No Hit
CGGGCAGCAGGACAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGA337940.11104608540697238No Hit
GGCACACCAAGCTCGCATTCCCCAGCCCCTTCGGGCACCTGTCTGAGGGC336740.11065176895290252No Hit
CGCATCAAGGTGGGACGGGATTACGTCCAGAAGGCGCAGACTAAAGAGCA332580.10928480524546036No Hit
CGGAACTGCGCTGCCTACCTGAAGCTGCGGAACTGGCAGTGGTGGCGGCT329150.10815771738091069No Hit
ATCACAGACACCGCCTACAGGAGTATGATGCAAGACCGAGAAGATCAATC327040.10746437761583785No Hit
TTCTCGAGGGCATCCGTATCTGCCGCCAGGGCTTCCCCAACAGGGTGGTC324680.10668888858950047No Hit
ATCAACAAGGCTCTGGACAAGACCAAGAGGCAGGGCGCCTCCTTCATCGG323860.10641943901255274No Hit
TCCTGGAGCAGGAGGAGTACCAGCGCGAGGGCATCGAGTGGAACTTCATC321020.10548622340458742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)