FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30432410
Sequences flagged as poor quality0
Sequence length35-71
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA1336800.062.74316817
TCGAGTC686400.062.1644315
GAGTCCG659750.061.9955217
GAGTCCT2300600.061.87998617
GGAGTCC2262750.061.72706216
ATTCGAG671500.061.60566713
CGAGTCC1035700.061.24046716
AGAGTCC660800.061.1645816
AATCGAG433650.061.12395513
AGTCCGA323000.061.04535718
AAAGGAG1107700.061.04197713
TGAGTCC518500.060.74106216
TTCGAGT451150.060.73612214
ATAGGAG547800.060.6812913
ATTGGAG1229850.060.49474713
AGGAGTC859100.060.28949415
AGTCCAG901950.060.23155218
AAGGAGT635400.059.98138814
AGTCCAT431550.059.63857718
ACGAGTC366700.059.35215815
Produced by FastQC (version 0.11.5)