FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27132716
Sequences flagged as poor quality0
Sequence length35-71
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA1240900.062.64241817
TCGAGTC644800.062.35492315
GAGTCCT2182250.062.24481217
ATTCGAG629900.062.11847713
GAGTCCG622300.062.1066917
GGAGTCC2175100.061.9637716
CGAGTCC961750.061.6725116
TGAGTCC479100.061.25868216
AGAGTCC582850.061.15720716
TTCGAGT420100.061.0908714
AATCGAG412900.061.0382113
AGTCCGA298800.060.88319818
AGTCCAT381200.060.86632518
ATTGGAG1189700.060.82067513
TTAGTCC296200.060.4323916
AAAGGAG1057500.060.33529313
ATAGGAG511400.060.22199213
AGTCCAG832000.060.13915318
ACGAGTC335850.059.91065615
ATTTGAG355100.059.8935213
Produced by FastQC (version 0.11.5)