FastQCFastQC Report
Thu 12 May 2022
EGAF00004976467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27216097
Sequences flagged as poor quality0
Sequence length35-231
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA16423766.034575787997816No Hit
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG8358713.0712375841400035No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT5351511.966303250609373No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA4874741.791123833810557No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC4625411.6995126082920708No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT4281081.5729955694969784No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA3968271.4580599121174502No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT3621981.3308227112800193No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT3509101.2893472565151425No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG3439701.2638476413425481No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG2557800.939811465251612No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG2519760.9258344427564319No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT2379120.8741591419225173No Hit
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT2221080.8160905658147823No Hit
CGCAAGAAAGTGGAGGCGCAGCTGCAGGAGCTGCAGGTCAAGTTCAACGA2212930.813096014465263No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC2142140.7870856721299899No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT2032630.746848455162399No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA1996090.7334225770873759No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG1896250.6967384044817301No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT1798530.6608331826565726No Hit
AGAAGGTGACCACCGAGGCGAAGCTGAAAAAGCTGGAGGAGGAGCAGATC1680660.6175242541206405No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG1679410.617064967103843No Hit
AGGAGGAGGAGGCCAAGCACAACCTGGAGAAGCAGATCGCCACCCTCCAT1546940.5683915662117165No Hit
CGTCTGGAGGCCCGCATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCA1325340.4869691638738648No Hit
TGAAGGTCAGAGAGAAGCAGCTGGCTGCGGAGAACAGGCTCACGGAGATG1284020.4717869722466083No Hit
GCACATCGACTCGGCCAACAAGAACCGGGACGAAGCCATCAAACAGCTGC1227660.451078639233245No Hit
GCAGAGACCCGTGCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAA1224120.44977793840167457No Hit
GAGGTCAACCTGCAGGCCATGAAGGCCCAGTTCGAGCGGGACCTGCAGGG1217480.4473382057684465No Hit
GAGATCTGCCATGACCTAGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTG1197710.4400741223107781No Hit
GAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAGGAGCTCCAGGCCGCCCT1135220.41711344576703996No Hit
CAGGGCAACACGGAGCTGATCAACGACCGGCTGAAGAAGGCCAACCTGCA1134980.4170252626598149No Hit
GAGGAGGCCAAGACCCACGAGGCCCAGATCCAGGAGATGAGGCAGAAGCA1128680.4147104560951557No Hit
GCACGAGATGCCCCCTCACATCTATGCCATCACAGACACCGCCTACAGGA1087900.39972667645915577No Hit
ATGGCAGAGCTCACGTGCCTCAACGAAGCCTCGGTGCTGCACAACCTCAA1085030.3986721534685888No Hit
CGGAAGAAGCTGGAGATGGACCTGAAGGACCTGGAGGCGCACATCGACTC1068000.39241482715174036No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG988870.3633401218403947No Hit
GCGCGAGAAGGAGGAGTTGATGCTGCGGCTGCAGGACTATGAGGAGAAGA942820.34641998814157665No Hit
CATCGAGTGGAACTTCATCGACTTTGGCCTCGACCTGCAGCCCTGCATCG941410.3459019123866291No Hit
GCCTCCATCACCGCCCTCGAGGCCAAGATTGCACAGCTGGAGGAGCAGCT939970.3453728137432785No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT914230.3359151754933854No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA846140.3108968931143948No Hit
GCTCTGCTGAACTCCAAGGAGGCCGCACTGAGCACTGCTCTCAGTGAGAA831680.30558386090408185No Hit
CTGAATGACAACATCGCCACACTGCTCCACCAGTCCTCTGACAAGTTTGT813780.29900687082354244No Hit
CTCGTGAGGAGATCCTGGCCCAGGCCAAAGAGAACGAGAAGAAGCTGAAG801190.2943809319903585No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA785730.28870047016660766No Hit
GGCTGCGGAGAACAGGCTCACGGAGATGGAGACGCTGCAGTCTCAGCTCA784570.2882742518150196No Hit
CCTCGGTGCTGCACAACCTCAAGGAGCGTTACTACTCAGGGCTCATCTAC783650.2879362165706567No Hit
ATCTAAGAGCCTCGCCAAGCTCAAGAACAAGCATGAGGCAATGATCACTG763250.28044065245652233No Hit
CCAGAGCGCGTGCAACCTGGAGAAGAAGCAGAAGAAGTTTGACCAGCTCC758490.27869168749655765No Hit
CCCTCGAGGCCAAGATTGCACAGCTGGAGGAGCAGCTGGACAACGAGACC751570.27614907457156695No Hit
GTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTACAAGGA744050.27338600387851353No Hit
GCTGCACTGCGGGCTAAGGAGGAGCTGGAGAGACAGGCGGTGGATCAGAT743740.2732721006983478No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT739060.2715525301074581No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGT736820.27072948777335704No Hit
CCCAGACTACGACTTGTGTAGCGTCTGCGAGGGAAAGGGCTTGCACCGGG719300.264292120945924No Hit
CTGCTGCAGGCCAACCCCATCCTGGAGGCCTTCGGGAACGCCAAGACCGT710960.2612277579698514No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG689310.2532729068389196No Hit
CGGAACTGCGCTGCCTACCTGAAGCTGCGGAACTGGCAGTGGTGGCGGCT685830.2519942517841556No Hit
CCGCACTGAGCACTGCTCTCAGTGAGAAGCGCACGCTGGAGGGCGAGCTG674010.24765123375331885No Hit
CCCAAGACCGTGCGCGACACGCTGCTGGCGCTGCACCAGCACGGCCACTC661820.24317226676551013No Hit
CGCATCAAGGTGGGACGGGATTACGTCCAGAAGGCGCAGACTAAAGAGCA660920.24284158011341597No Hit
GCCGATGCCATGAACCGCGAAGTCAGCTCCCTAAAGAACAAGCTCAGGCG650410.238979894876183No Hit
CCCACGTCCTCCTCGTGCAGGGGAGGCCCGCCCTGGCCCCACGGCAGAAT633300.23269317419025953No Hit
GAAGAAGCTGAAGGATGTGCTGCTGCAGGTGGATGACGAGCGGAGGAACG624660.22951858233015557No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG623140.22896008931772988No Hit
CGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCCGACAACA622650.22878004880714525No Hit
ATGGAGGCCGAGATGATCCAGTTGCAGGAGGAACTGGCAGCCGCGGAGCG619600.2276593884861595No Hit
CAGCTCGGCAACATCGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTC599380.22022996170244397No Hit
GACCCGTGCTGAATTTGCAGAGAGAACGGTTGCAAAACTGGAAAAGACAA591880.21747423960165926No Hit
CGGGCAGCAGGACAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGA589590.21663282578688633No Hit
GGCACACCAAGCTCGCATTCCCCAGCCCCTTCGGGCACCTGTCTGAGGGC578280.21247719685890304No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG574430.21106259284716689No Hit
CCCCGAGCCTGAGGCGGAAGCCGAGGCTGCGGCGGGTCCGGGACCCTGCG572520.2103608022855004No Hit
CCCAGGCCAAAGAGAACGAGAAGAAGCTGAAGAGCATGGAGGCCGAGATG572280.21027261917827525No Hit
GCTGGACGACCTGCTGGTGGACCTGGACCACCAGCGCCAGAGCGCGTGCA558210.20510288451720315No Hit
AAAACAGAGTTGGAGGACACGCTGGATTCCACAGCTGCCCAGCAGGAGCT543650.1997531093455465No Hit
AAAGTCCTCTCGGGAGAAGCAGCGCCTGTTCCCGGGGCAGATCCAGGTTC536310.1970561759829119No Hit
GCTGGAGGACGCCACTGAGACGGCCGATGCCATGAACCGCGAAGTCAGCT524200.1926066033641782No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA505860.18586794425372602No Hit
CCAGTATCTGGCGTACGTGGCGTCCTCGCACAAGAGCAAGAAGGACCAGG494900.181840915690446No Hit
CTGCAGGTCAAGTTCAACGAGGGAGAGCGCGTGCGCACAGAGCTGGCCGA476840.17520513687175643No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT473320.17391178463245482No Hit
CACTGCTCGACCTGAGAGACCCATGGCATTCCTCAGGGAATACTTTGAGA460960.16937035461036165No Hit
TGAAAAAGCTGGAGGAGGAGCAGATCATCCTGGAGGACCAGAACTGCAAG459700.16890739329742982No Hit
CAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGATTATGGGCATCC456040.1675626009122469No Hit
TTCACCTGTCCAGCATCCGACCACCGAGGCTGGAGGGGGAGAACACCCAG435770.16011480264785946No Hit
CCAGTTCGAGCGGGACCTGCAGGGCCGGGACGAGCAGAGCGAGGAGAAGA435350.15996048221021553No Hit
CTGATGGCTACGCTGAGGAACACGAACCCCAACTTTGTCCGCTGCATCAT429140.15767874431076578No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGGGAAGTGAAACTGT415920.152821324821116No Hit
GTCCGACAGCAAGTCCAGCAAGCTCACCAAGGACTTCTCCGCGCTGGAGT415610.15270742164095022No Hit
TTCTCGAGGGCATCCGTATCTGCCGCCAGGGCTTCCCCAACAGGGTGGTC410810.150943759496448No Hit
GGGCGCCTCCTTCATCGGGATCCTGGACATTGCCGGCTTCGAGATCTTTG399850.14691673093316798No Hit
CTGAGGAACACGAACCCCAACTTTGTCCGCTGCATCATCCCCAACCACGA397880.1461928945946952No Hit
GAATCCTCACCCCGCGCATCAAGGTGGGACGGGATTACGTCCAGAAGGCG379330.13937707526542106No Hit
TCCAGGCCCAGATCGCGGAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAG373210.13712840603118073No Hit
CAGAATCCGAAGGGCCAAGCTCTCTGGACCCCTCCCAGGAGGGACCCACA364720.13400892861309247No Hit
AGTTGCAGGAGGAACTGGCAGCCGCGGAGCGTGCCAAGCGCCAGGCCCAG360440.132436329867578No Hit
GCAAGCCGGGTGGGAATGTTGAGGGCGCCACGCAGTCTCTGGCGGAGCAG351480.1291441605311739No Hit
GACGTCATCATAGGGTTCCAGGCCTGCTGCAGGGGCTACCTGGCCAGGAA339300.12466886783949954No Hit
CACTGCTCCACCAGTCCTCTGACAAGTTTGTCTCGGAGCTGTGGAAGGAT334240.12280967399550347No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC332950.1223356897941685No Hit
GTCCAGCAAGCTCACCAAGGACTTCTCCGCGCTGGAGTCCCAGCTGCAGG331300.12172943093199586No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG329350.12101294318579185No Hit
CAGAGATATGAGATCCTGACTCCAAACTCCATTCCCAAGGGTTTCATGGA327950.12049854172697871No Hit
CCCCAAGTTCCAGAAGCCCAAGCAGCTGAAGGACAAAGCTGATTTCTGCA327860.1204654730617693No Hit
CAACAAGCAGTTCCGCACGGAGATGGAGGACCTTATGAGCTCCAAGGATG324980.11940727577506798No Hit
GAAGCCGAAATAAATGAGAGATGAGTTGGGATCAAGTGGATTGAGGAGGC321370.11808085487055693No Hit
CCATGAAGGTCCTCCAGCGGAACTGCGCTGCCTACCTGAAGCTGCGGAAC317550.11667727374722392No Hit
CGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTAG309860.11385174001988603No Hit
GAGACCTGAAGATCACCGACGTCATCATAGGGTTCCAGGCCTGCTGCAGG287390.1055955966059351No Hit
CGAGGAGGAACAGGAGGATGAGCAGCCACCCTGGGCCAGCCCGAGCCCCA287030.10546332194509743No Hit
CCCCAGAGGAGCCCGAAACCTCCCAGGCTTGGTGCAGGAAGGAGAGCCCT286370.10522081840022837No Hit
GCTGGAGAGACAGGCGGTGGATCAGATAAAGAGCCAGGAGCAGCTGGCTG277080.10180739729138973No Hit
CGCACGGAGATGGAGGACCTTATGAGCTCCAAGGATGATGTGGGCAAGAG275980.10140322471660797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)