FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27216097
Sequences flagged as poor quality0
Sequence length35-71
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA1150350.062.58816517
GAGTCCT1955450.062.49305317
TCGAGTC569750.062.05737315
ATTCGAG537700.062.0303313
GGAGTCC1934800.061.98461516
GAGTCCG549100.061.53896317
AATCGAG351850.061.47889713
AGAGTCC540700.061.36798516
ATTGGAG1011100.061.30315813
TTCGAGT364750.061.1973314
TTAGTCC267750.060.89389416
CGAGTCC867650.060.88926316
TGAGTCC454800.060.7211516
AGTCCAT332700.060.43851518
AAAGGAG933000.060.42415613
ATTTGAG318000.060.14931513
ATAGGAG446200.060.09984613
AGTCCGA256650.059.98658818
GATTCGA213550.059.77295712
AGTCCAG818700.059.4970318
Produced by FastQC (version 0.11.5)