FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23407889
Sequences flagged as poor quality0
Sequence length35-71
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA961750.063.1563817
TCGAGTC530850.062.20727515
ATTCGAG518850.062.02071813
GAGTCCT1777200.062.01729617
GGAGTCC1679900.061.94457216
CGAGTCC796300.061.9350616
GAGTCCG522650.061.7075317
AATGGAG780500.061.68243813
AGAGTCC518350.061.45268216
AATCGAG340250.061.4486913
AAAGGAG859700.061.36387313
TGAGTCC395100.060.7770516
ATTGGAG905750.060.74503313
AGTCCAT331450.060.68309818
ATAGGAG422950.060.6496813
AGGAGTC673900.060.57330315
AGTCCAG625850.060.3297618
TTCGAGT350950.060.2102914
AAACGAG458950.059.9761713
ACGAGTC288150.059.89748415
Produced by FastQC (version 0.11.5)