FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25557015
Sequences flagged as poor quality0
Sequence length35-71
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCT1855600.062.3828517
GAGTCCA1065000.062.340117
ATTCGAG528650.062.2337413
TCGAGTC560350.062.05743415
GGAGTCC1808700.061.8852416
CGAGTCC839300.061.50591716
GAGTCCG551350.061.49502617
TGAGTCC421250.061.40622316
ATTGGAG948650.061.3435313
AATCGAG356550.061.02583313
TTCGAGT352650.060.84860614
AGAGTCC539250.060.74460216
AAAGGAG901300.060.70515413
AGTCCAT354950.060.58418
AATGGAG832350.060.4970213
AGTCCGA273900.060.4812518
GATTCGA188050.060.4483412
ATAGGAG424450.060.2767813
AGTCCAG691500.060.17756318
TTAGTCC261800.059.7095716
Produced by FastQC (version 0.11.5)