FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26196289
Sequences flagged as poor quality0
Sequence length35-71
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA1191950.062.4707217
TCGAGTC615700.062.0510615
GAGTCCT2103750.061.7361817
CGAGTCC930850.061.62050216
GGAGTCC2042950.061.6080116
AATCGAG388300.061.60382513
GAGTCCG596150.061.46596517
TTCGAGT399300.061.44344714
ATTGGAG1091900.061.2977413
AGTCCGA282350.061.08816518
ATTCGAG609350.061.02810713
AGTCCAT390600.060.76410318
AGAGTCC597650.060.38335816
TGAGTCC467000.060.3362316
TTAGTCC278850.060.33481216
AAAGGAG1007000.060.29220213
AATGGAG946450.060.04456713
TATTCGA185450.059.8704612
ACGAGTC333550.059.75834715
ATAGGAG487450.059.7465613
Produced by FastQC (version 0.11.5)