FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004976483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25459576
Sequences flagged as poor quality0
Sequence length35-231
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG5313612.0870771767762353No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC4175091.6398898394851509No Hit
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA3932061.5444326331279044No Hit
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT3674031.4430837339946274No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT3285471.290465324324333No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG3118011.2246904661727283No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA3097501.2166345582503024No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG2840741.115784489105396No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG2379130.9344735356158327No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT2301980.9041705957711157No Hit
GTGATTGAAGCTGAGTACATTTTGCTGGTGTATTTTTAGGTAAAATGCTT2286920.8982553362239811No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC2263380.8890093063607971No Hit
GATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGTATTATCAAGTAAGA2220900.8723240324190787No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT2195290.8622649489528027No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT2138340.8398961553798068No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA2083030.8181715202169904No Hit
CCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAGTGTTGGGGCAATC2024470.795170351619367No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG1969390.773536055745783No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT1953470.7672830058128226No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA1940080.7620236880614194No Hit
TCAAGAGTAATTACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGA1931300.7585750838898495No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG1928860.7576167018649486No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT1890490.7425457517438625No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG1798390.7063707580990352No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG1763490.6926627529068041No Hit
GCATGATGTGCTGTTAGAATCAGATGTTACTGCTAAAATTTACATGTTGT1743400.6847718123821073No Hit
AGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT1640670.6444215724566662No Hit
GCAGAGACCCGTGCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAA1527080.5998057469613791No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG1511490.5936823142694914No Hit
ATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATATGAGTGCTTGGCT1480760.5816121996689968No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG1471680.5780457616419064No Hit
GGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAA1378430.5414190715509166No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT1377150.540916313767362No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA1373690.539557296633691No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA1373570.5395101630914828No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT1362590.5351974439794284No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT1319590.5183079246881409No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG1302760.5116974453934346No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT1242110.4878753676023513No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC1175770.4618183743515603No Hit
GAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAG1165690.4578591568060678No Hit
TTTAGGTAAAATGCTTTTTGTTCATTTCTGGTGGTGGGAGGGGACTGAAG1156050.45407276224867216No Hit
ACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGATTATTTTTTAAA1141630.4484088815933148No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA1126000.44226973772069106No Hit
GAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGG1033070.40576873707558997No Hit
AGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACG993760.39032857420720596No Hit
CTCTGAAGGCTCTATGAAAGGAATAGCATGATGTGCTGTTAGAATCAGAT871610.3423505560343974No Hit
GAAATTAAACTTCTGTCTGACAAACTGAAAGAGGCTGAGACCCGTGCTGA817490.3210933284984793No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGT772340.3033593332426275No Hit
AGAAGGTGACCACCGAGGCGAAGCTGAAAAAGCTGGAGGAGGAGCAGATC730390.2868822324456621No Hit
GGGAACCACTAGTTCTTTCAGATGGTATTCTTCAGACTATAGAAGGAGCT705220.2769959719674829No Hit
CGCAAGAAAGTGGAGGCGCAGCTGCAGGAGCTGCAGGTCAAGTTCAACGA685280.2691639483705463No Hit
ATGGCAGAGCTCACGTGCCTCAACGAAGCCTCGGTGCTGCACAACCTCAA679680.2669643830674949No Hit
CGGAAGAAGCTGGAGATGGACCTGAAGGACCTGGAGGCGCACATCGACTC674910.26509082476471724No Hit
CGGGTCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAACATGCCAGA664290.26091950627928756No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGGGAAGTGAAACTGT658090.25848427326519496No Hit
GAAGCCGAAATAAATGAGAGATGAGTTGGGATCAAGTGGATTGAGGAGGC625130.2455382603386639No Hit
ATTCTTCAGACTATAGAAGGAGCTTCCAGTTGAATTCACCAGTGGACAAA599880.23562057749901255No Hit
AGGAGGAGGAGGCCAAGCACAACCTGGAGAAGCAGATCGCCACCCTCCAT592090.2325608250506607No Hit
GCAACAGGTGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC566920.22267456457248147No Hit
GACCCGTGCTGAATTTGCAGAGAGAACGGTTGCAAAACTGGAAAAGACAA562600.2209777570529847No Hit
CGTTCTACATCTCATTCAGGTCAAGCAGGGTCTGGTCCAGCATCCTTTGT554140.2176548423273035No Hit
TGAAGGTCAGAGAGAAGCAGCTGGCTGCGGAGAACAGGCTCACGGAGATG548150.21530209301207529No Hit
GAGATCTGCCATGACCTAGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTG490050.19248160299291708No Hit
AGAGTGAATTATGACACAACAGAATCCAAGCTCCGGAGAGAGTTTGAGGT486960.1912679142810548No Hit
CGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCCGACAACA457880.17984588588592362No Hit
TATGAGGAAGAAATCAAGATTCTTACTGATAAACTCAAGGAGGCAGAGAC452910.17789377167946552No Hit
AAAGTCCTCTCGGGAGAAGCAGCGCCTGTTCCCGGGGCAGATCCAGGTTC451440.17731638578741454No Hit
CTGAAGAGATTGTGGAAATGTACAAGGGCAAGAAGAGGCACGAGATGCCC449710.17663687722057902No Hit
GAGCTGACAGCTCGAATCTCCCAGCTGGAGATGGCCCGACAGAAGAAGGA446910.1755370945690533No Hit
CAGGGCAACACGGAGCTGATCAACGACCGGCTGAAGAAGGCCAACCTGCA430600.1691308606239161No Hit
GCACATCGACTCGGCCAACAAGAACCGGGACGAAGCCATCAAACAGCTGC424330.16666813304353537No Hit
CGGTCAGAAAATGAAGAGTTTGTTGAAGTGGGAAGATTGGGGCCTTCTGA418700.1644567843549319No Hit
AAAACAGAGTTGGAGGACACGCTGGATTCCACAGCTGCCCAGCAGGAGCT418500.1643782284512515No Hit
GCTGGACGACCTGCTGGTGGACCTGGACCACCAGCGCCAGAGCGCGTGCA409810.16096497443633784No Hit
GTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTACAAGGA408390.16040722752020692No Hit
CGTCTGGAGGCCCGCATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCA406580.15969629659189924No Hit
GAGGTCAACCTGCAGGCCATGAAGGCCCAGTTCGAGCGGGACCTGCAGGG385810.151538265994689No Hit
TGAAAAAGCTGGAGGAGGAGCAGATCATCCTGGAGGACCAGAACTGCAAG379790.14917373329390873No Hit
CCAAGCAGCTGAAGGACAAAGCTGATTTCTGCATTATCCACTATGCCGGC375610.14753191490698825No Hit
ATCTAAGAGCCTCGCCAAGCTCAAGAACAAGCATGAGGCAATGATCACTG367980.14453500718158072No Hit
CGCACGGAGATGGAGGACCTTATGAGCTCCAAGGATGATGTGGGCAAGAG367540.14436218419348382No Hit
CATCGAGTGGAACTTCATCGACTTTGGCCTCGACCTGCAGCCCTGCATCG367400.14430719506090753No Hit
CGCTGTGGTCACCAGGGAGGAGCCCATTTACATCATCACCGAGTACATGG355940.1398059417800202No Hit
CCAGGAAATAGCGCTGGAGCTTTCCTTATTAGAGAAAGTGAAACATTAAA355450.13961347981600322No Hit
CCCGGGAGTCCATCAAGTTGGTGAAAAGGCTTGGCGCTGGGCAGTTTGGG354950.1394170900568022No Hit
CTCGTGAGGAGATCCTGGCCCAGGCCAAAGAGAACGAGAAGAAGCTGAAG352970.13863938661036618No Hit
GAGGAGGCCAAGACCCACGAGGCCCAGATCCAGGAGATGAGGCAGAAGCA352380.13840764669450897No Hit
CAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGATTATGGGCATCC352040.13827410165825227No Hit
CCCCCACTCTGAACTCAAGTCACCGTGGAGCTCCGCCGCCCCGAAACTTT349180.13715075223562245No Hit
GCACGAGATGCCCCCTCACATCTATGCCATCACAGACACCGCCTACAGGA339470.1333368631119387No Hit
GGCTGCGGAGAACAGGCTCACGGAGATGGAGACGCTGCAGTCTCAGCTCA337890.1327162714728635No Hit
CCCAAGACCGTGCGCGACACGCTGCTGGCGCTGCACCAGCACGGCCACTC336670.13223708046041302No Hit
GAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAGGAGCTCCAGGCCGCCCT326730.12833285204749678No Hit
CCCCAAGTTCCAGAAGCCCAAGCAGCTGAAGGACAAAGCTGATTTCTGCA325250.12775153836026176No Hit
GCACAATATCATGGATTTTGCCATGCCCTATTTCATCCAGGTCATGAAGG325040.12766905466139736No Hit
AAAAAGAAGGCTTCATCCCCAGCAACTATGTGGCCAAACTCAACACCTTA324620.1275040872636685No Hit
TCCAGGCCCAGATCGCGGAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAG321670.1263453876843825No Hit
GAAGAAGCTGAAGGATGTGCTGCTGCAGGTGGATGACGAGCGGAGGAACG318260.12500600952663155No Hit
AGTTGCAGGAGGAACTGGCAGCCGCGGAGCGTGCCAAGCGCCAGGCCCAG317170.12457787985157333No Hit
CTGAATGACAACATCGCCACACTGCTCCACCAGTCCTCTGACAAGTTTGT316560.1243382843453481No Hit
GCTGGAGATGGCCCGACAGAAGAAGGAGAGTGAGGCTGTGGAGTGGCAGC314280.12344274704339145No Hit
GGACATAGGACTCAAAGTTTCAGAGACCATTGCAATGAATCCCCAATAAT303530.11922036722056958No Hit
GCGCGAGAAGGAGGAGTTGATGCTGCGGCTGCAGGACTATGAGGAGAAGA302270.11872546502738303No Hit
CAGAGACCATTGCAATGAATCCCCAATAATTGCAGAACTAAACTCATTTA295140.11592494706117651No Hit
TAGAAAGCTGTCTCCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAG290390.11405924434876684No Hit
CCCTCGAGGCCAAGATTGCACAGCTGGAGGAGCAGCTGGACAACGAGACC287730.11301445082981744No Hit
CTGCAGGTCAAGTTCAACGAGGGAGAGCGCGTGCGCACAGAGCTGGCCGA286090.1123702924196381No Hit
GCGCAGGCTGAGAATATGGAGAGACTATGTGGCTCCCACAGCTAATTTGG285500.1121385525037809No Hit
ATGGAGGCCGAGATGATCCAGTTGCAGGAGGAACTGGCAGCCGCGGAGCG281960.1107481130086377No Hit
CACTGCTCCACCAGTCCTCTGACAAGTTTGTCTCGGAGCTGTGGAAGGAT280170.11004503767069805No Hit
CTGAGGAACACGAACCCCAACTTTGTCCGCTGCATCATCCCCAACCACGA278740.10948336295938314No Hit
GCGGAGTCTGGGTCAGAGGGAGGAGCAGCAGCAGGGTGGGACTGGGGCGT278270.10929875658573418No Hit
CTCATCCTGCTCATCCTGCTCATTCTCAGCAGGCTCTGCCACATGAGGTG275000.10801436756055953No Hit
CACCATTGGCTCCTGTTTGAAATGAGCAGGCACTCCTTGGAGCAAAAGCC274530.10782976118691057No Hit
TGTCCCGGAAGACCCATGACGCCTCGGGCCAGCTAGTCCTCATCAGCCAG267220.1049585429073917No Hit
GACGTCATCATAGGGTTCCAGGCCTGCTGCAGGGGCTACCTGGCCAGGAA267050.10489177038926335No Hit
AGGCAAATCGGATGAGTGTAGATGCTGTAGAAATTGAAACACTCAGAAAA265850.10442043496718091No Hit
CCAGTATCTGGCGTACGTGGCGTCCTCGCACAAGAGCAAGAAGGACCAGG264570.10391767718362631No Hit
CCCAGGCCAAAGAGAACGAGAAGAAGCTGAAGAGCATGGAGGCCGAGATG263740.10359167018335262No Hit
GCTGGAGGACGCCACTGAGACGGCCGATGCCATGAACCGCGAAGTCAGCT261460.10269613288139598No Hit
GCCTCCATCACCGCCCTCGAGGCCAAGATTGCACAGCTGGAGGAGCAGCT259700.1020048409290084No Hit
CCCTATGATGGCATCCACCCGGACGACTTGTCTTTCAAGAAAGGAGAGAA255890.10050835096389665No Hit
CTATTACATCTCTCCACGAATCACTTTTCCCTGTATCAGCGACATGATTA255220.10024518868656729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)