FastQCFastQC Report
Wed 11 May 2022
EGAF00004976485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20957401
Sequences flagged as poor quality0
Sequence length35-231
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG5464962.6076515880952984No Hit
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA4726432.255255792452509No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT3483981.6624103341821823No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA3474671.657967989446783No Hit
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT3200801.5272886175151204No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG3121011.489216148510018No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG3017391.439772994752546No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC2523501.2041092309108368No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG2517061.201036330793117No Hit
GTGATTGAAGCTGAGTACATTTTGCTGGTGTATTTTTAGGTAAAATGCTT2462261.1748880502882968No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC2457451.1725929183680743No Hit
CCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAGTGTTGGGGCAATC2345711.1192752383752165No Hit
GATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGTATTATCAAGTAAGA2268711.0825340413155238No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG2253471.075262147248125No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT2152991.027317270877243No Hit
TCAAGAGTAATTACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGA2100141.0020994492589994No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT2020160.9639363201572562No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA1995200.9520264464090753No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT1995170.9520121316569741No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT1982390.9459140472618718No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT1945300.9282162420807809No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA1911760.9122123492316627No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG1835860.8759960264156801No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG1804380.8609750798775095No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG1759680.8396460992467529No Hit
AGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT1735360.8280416068767305No Hit
GCATGATGTGCTGTTAGAATCAGATGTTACTGCTAAAATTTACATGTTGT1708890.815411223939457No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG1660610.7923740162246263No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA1561600.7451305627067021No Hit
GGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAA1543720.7365989704543994No Hit
ATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATATGAGTGCTTGGCT1511280.7211199518489912No Hit
TTTAGGTAAAATGCTTTTTGTTCATTTCTGGTGGTGGGAGGGGACTGAAG1483300.7077690597226249No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC1466260.6996382805291553No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT1425030.6799650395581016No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT1355480.6467786726035352No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA1329250.634262807683071No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT1328660.6339812842250812No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG1317790.6287945723804206No Hit
GAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAG1231570.5876539748416323No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA1208400.5765982146354884No Hit
ACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGATTATTTTTTAAA1145660.5466612964078895No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT1139820.5438746913321933No Hit
GAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGG1057670.5046761284951317No Hit
AGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACG965260.46058192043946666No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTTTGTCTGTTCTG948400.45253702975860416No Hit
GGGAACCACTAGTTCTTTCAGATGGTATTCTTCAGACTATAGAAGGAGCT809480.3862501843620781No Hit
CTCTGAAGGCTCTATGAAAGGAATAGCATGATGTGCTGTTAGAATCAGAT797650.3806054004501799No Hit
ATTCTTCAGACTATAGAAGGAGCTTCCAGTTGAATTCACCAGTGGACAAA720900.34398349299133035No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG703000.33544235757096025No Hit
GCAACAGGTGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC615510.2936957688598887No Hit
GAAGCCGAAATAAATGAGAGATGAGTTGGGATCAAGTGGATTGAGGAGGC531140.2534379143673397No Hit
GCAGAGACCCGTGCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAA526820.2513765900647699No Hit
CGGGTCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAACATGCCAGA476810.22751389831210464No Hit
GCTCTGCTGAACTCCAAGGAGGCCGCACTGAGCACTGCTCTCAGTGAGAA469260.22391135236664128No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGT463210.22102454402623684No Hit
ATGGCAGAGCTCACGTGCCTCAACGAAGCCTCGGTGCTGCACAACCTCAA440200.21004512916463258No Hit
AGAAGGTGACCACCGAGGCGAAGCTGAAAAAGCTGGAGGAGGAGCAGATC439560.20973974778647408No Hit
CGCAAGAAAGTGGAGGCGCAGCTGCAGGAGCTGCAGGTCAAGTTCAACGA434900.20751618962675766No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGGGAAGTGAAACTGT412960.1970473342567621No Hit
GCGCGAGAAGGAGGAGTTGATGCTGCGGCTGCAGGACTATGAGGAGAAGA408570.19495260886595622No Hit
TAGAAAGCTGTCTCCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAG384360.18340060392030483No Hit
AGAGTGAATTATGACACAACAGAATCCAAGCTCCGGAGAGAGTTTGAGGT381320.181950042374052No Hit
AGGAGGAGGAGGCCAAGCACAACCTGGAGAAGCAGATCGCCACCCTCCAT375840.17933521432356997No Hit
ACAGAGGAGAAGAGCAGCTCACAGCCAAGCAGCTGCTGCTCTGATCCCAG351340.1676448334409405No Hit
TGAAGGTCAGAGAGAAGCAGCTGGCTGCGGAGAACAGGCTCACGGAGATG328950.15696125678942727No Hit
CTGAAGAGATTGTGGAAATGTACAAGGGCAAGAAGAGGCACGAGATGCCC318440.15194632196998092No Hit
CGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCCGACAACA317470.15148347831870945No Hit
GAGATCTGCCATGACCTAGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTG309730.1477902722766053No Hit
CGGAAGAAGCTGGAGATGGACCTGAAGGACCTGGAGGCGCACATCGACTC300010.1431522925958233No Hit
GCTGGAGAGACAGGCGGTGGATCAGATAAAGAGCCAGGAGCAGCTGGCTG295480.1409907650285453No Hit
GTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTACAAGGA286980.13693491859987791No Hit
CAGGGCAACACGGAGCTGATCAACGACCGGCTGAAGAAGGCCAACCTGCA285960.13644821702843785No Hit
GCTGGACGACCTGCTGGTGGACCTGGACCACCAGCGCCAGAGCGCGTGCA278890.1330747071165933No Hit
AAAGTCCTCTCGGGAGAAGCAGCGCCTGTTCCCGGGGCAGATCCAGGTTC272700.13012109659971674No Hit
AAAACAGAGTTGGAGGACACGCTGGATTCCACAGCTGCCCAGCAGGAGCT271410.12950556225936602No Hit
CGTCTGGAGGCCCGCATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCA267250.12752058330133587No Hit
CCCAGACTACGACTTGTGTAGCGTCTGCGAGGGAAAGGGCTTGCACCGGG265390.1266330686710628No Hit
CCAGCGCGTGCTCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTG264540.12622748402819606No Hit
ACCGTGGAGAGAGAGAAAGAGCAGATGATGCGCGAGAAGGAGGAGTTGAT263420.1256930666164187No Hit
GAGGTCAACCTGCAGGCCATGAAGGCCCAGTTCGAGCGGGACCTGCAGGG251440.11997670894401459No Hit
GGAATTGACAATGGCCATGTCCTACGTGAAGGATGACATCTTCCGAATCT249470.11903670688937049No Hit
GCTGCACTGCGGGCTAAGGAGGAGCTGGAGAGACAGGCGGTGGATCAGAT245230.11701355525907053No Hit
TGAAAAAGCTGGAGGAGGAGCAGATCATCCTGGAGGACCAGAACTGCAAG238270.11369253277159701No Hit
GAAATTAAACTTCTGTCTGACAAACTGAAAGAGGCTGAGACCCGTGCTGA237620.1133823798094048No Hit
ATCTAAGAGCCTCGCCAAGCTCAAGAACAAGCATGAGGCAATGATCACTG235980.11259984002787368No Hit
CAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGATTATGGGCATCC233960.11163598005306097No Hit
CAGAATCCGAAGGGCCAAGCTCTCTGGACCCCTCCCAGGAGGGACCCACA231400.11041445454042705No Hit
CGCACGGAGATGGAGGACCTTATGAGCTCCAAGGATGATGTGGGCAAGAG229900.10969871693536808No Hit
CATCGAGTGGAACTTCATCGACTTTGGCCTCGACCTGCAGCCCTGCATCG229460.10948876723788413No Hit
GAGGAGGCCAAGACCCACGAGGCCCAGATCCAGGAGATGAGGCAGAAGCA228650.10910226893115228No Hit
CCAAGCAGCTGAAGGACAAAGCTGATTTCTGCATTATCCACTATGCCGGC226190.10792845925885561No Hit
CTCGTGAGGAGATCCTGGCCCAGGCCAAAGAGAACGAGAAGAAGCTGAAG225690.10768988005716931No Hit
GGCTGCGGAGAACAGGCTCACGGAGATGGAGACGCTGCAGTCTCAGCTCA225200.10745607243951671No Hit
CGGTCAGAAAATGAAGAGTTTGTTGAAGTGGGAAGATTGGGGCCTTCTGA225140.10742744293531437No Hit
GCACGAGATGCCCCCTCACATCTATGCCATCACAGACACCGCCTACAGGA222540.10618683108654552No Hit
CCGCACTGAGCACTGCTCTCAGTGAGAAGCGCACGCTGGAGGGCGAGCTG218360.10419230896044791No Hit
TCCAGGCCCAGATCGCGGAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAG214020.10212144148981069No Hit
AGTTGCAGGAGGAACTGGCAGCCGCGGAGCGTGCCAAGCGCCAGGCCCAG212560.10142479022088664No Hit
CAACAGACAGAGGCTGCAGAATCTGGAGTTTGCTCTGAATCTCCTCAGAC210350.10037027014943313No Hit
GAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAGGAGCTCCAGGCCGCCCT209700.10006011718724092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)