FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004976487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9925946
Sequences flagged as poor quality0
Sequence length35-231
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG2626672.6462666631472707No Hit
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA2197852.2142473876041637No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA1611571.6235933582552233No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG1609681.6216892576284416No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT1562051.573703906912248No Hit
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT1494141.5052872542324933No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC1317641.3274704496679712No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG1287961.2975690176029568No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG1139451.1479510366064858No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT1130281.1387126224543231No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG1124081.1324663664299603No Hit
GTGATTGAAGCTGAGTACATTTTGCTGGTGTATTTTTAGGTAAAATGCTT1117001.1253335450343978No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT1060821.0687344057684778No Hit
GATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGTATTATCAAGTAAGA1045791.0535922722126434No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA1034841.0425605781050995No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC1022541.0301688121212829No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA978830.9861327071495251No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT978180.9854778577276161No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT972230.979483466865526No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG951970.9590723141149468No Hit
TCAAGAGTAATTACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGA944130.9511738226260752No Hit
CCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAGTGTTGGGGCAATC938410.9454111477132758No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT904770.9115201714778621No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG817260.8233572900759283No Hit
AGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT813110.8191763283822016No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG804380.8103811969156391No Hit
GCATGATGTGCTGTTAGAATCAGATGTTACTGCTAAAATTTACATGTTGT793670.7995912933638768No Hit
ATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATATGAGTGCTTGGCT781490.7873204226579512No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT751170.7567742157775188No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG741870.7474048317409746No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA707760.7130403490004882No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG696140.7013336562580533No Hit
GGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAA685000.6901105446271821No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT640940.6457218284282424No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC623420.6280711178561721No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA620240.6248673929920634No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT613670.618248376527537No Hit
TTTAGGTAAAATGCTTTTTGTTCATTTCTGGTGGTGGGAGGGGACTGAAG594610.5990461765558669No Hit
GAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAG580800.5851331449919231No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT573580.5778592791054877No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA544030.5480888169248553No Hit
GAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGG524980.528896691559676No Hit
ACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGATTATTTTTTAAA507400.5111855333486601No Hit
AGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACG470680.47419157831404685No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTTTGTCTGTTCTG396080.3990350138918749No Hit
CTCTGAAGGCTCTATGAAAGGAATAGCATGATGTGCTGTTAGAATCAGAT376440.379248486743732No Hit
GGGAACCACTAGTTCTTTCAGATGGTATTCTTCAGACTATAGAAGGAGCT365880.36860970228933343No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG349380.3519866015793356No Hit
CGGGTCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAACATGCCAGA310990.3133101872607407No Hit
ATTCTTCAGACTATAGAAGGAGCTTCCAGTTGAATTCACCAGTGGACAAA309810.31212138369481357No Hit
GCAGAGACCCGTGCTGAGTTTGCTGAGAGATCGGTAGCCAAGCTGGAAAA309610.3119198915649954No Hit
GAAGCCGAAATAAATGAGAGATGAGTTGGGATCAAGTGGATTGAGGAGGC271540.27356586465410954No Hit
AGAAGGTGACCACCGAGGCGAAGCTGAAAAAGCTGGAGGAGGAGCAGATC232770.23450661528886013No Hit
GCTCTGCTGAACTCCAAGGAGGCCGCACTGAGCACTGCTCTCAGTGAGAA227940.22964058035375165No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGT222890.22455290407584327No Hit
CGCAAGAAAGTGGAGGCGCAGCTGCAGGAGCTGCAGGTCAAGTTCAACGA218950.2205835091184256No Hit
ATGGCAGAGCTCACGTGCCTCAACGAAGCCTCGGTGCTGCACAACCTCAA218470.220099928006862No Hit
GCGCGAGAAGGAGGAGTTGATGCTGCGGCTGCAGGACTATGAGGAGAAGA207780.20933017366808163No Hit
AGGAGGAGGAGGCCAAGCACAACCTGGAGAAGCAGATCGCCACCCTCCAT203820.20534062949768214No Hit
AGAGTGAATTATGACACAACAGAATCCAAGCTCCGGAGAGAGTTTGAGGT196390.19785519687493766No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGGGAAGTGAAACTGT195970.19743206340231953No Hit
GCAACAGGTGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC194920.1963742297207742No Hit
TGAAGGTCAGAGAGAAGCAGCTGGCTGCGGAGAACAGGCTCACGGAGATG188220.189624243371866No Hit
CGGAAGAAGCTGGAGATGGACCTGAAGGACCTGGAGGCGCACATCGACTC165310.1665433199011963No Hit
ACAGAGGAGAAGAGCAGCTCACAGCCAAGCAGCTGCTGCTCTGATCCCAG165210.16644257383628724No Hit
CGTCTTCAAGAAGGAGCGGAACACTGACCAGGCGTCCATGCCCGACAACA164350.16557615767806919No Hit
GAGATCTGCCATGACCTAGAGGCCAGGGTGGAGGAGGAGGAGGAGCGCTG162430.1636418332318149No Hit
GCTGGAGAGACAGGCGGTGGATCAGATAAAGAGCCAGGAGCAGCTGGCTG154630.15578364016890683No Hit
CAGGGCAACACGGAGCTGATCAACGACCGGCTGAAGAAGGCCAACCTGCA154460.1556123718585614No Hit
TAGAAAGCTGTCTCCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAG149850.1509679782662529No Hit
CTGAAGAGATTGTGGAAATGTACAAGGGCAAGAAGAGGCACGAGATGCCC148880.14999074143663485No Hit
AAAGTCCTCTCGGGAGAAGCAGCGCCTGTTCCCGGGGCAGATCCAGGTTC147050.14814708844879873No Hit
GTGCTGCTGCAGGTGGATGACGAGCGGAGGAACGCCGAGCAGTACAAGGA146520.14761313430478062No Hit
GCTGGACGACCTGCTGGTGGACCTGGACCACCAGCGCCAGAGCGCGTGCA143970.14504410964959913No Hit
CGTCTGGAGGCCCGCATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCA140220.14126613221550874No Hit
GAAATTAAACTTCTGTCTGACAAACTGAAAGAGGCTGAGACCCGTGCTGA138600.13963404596398166No Hit
AAAACAGAGTTGGAGGACACGCTGGATTCCACAGCTGCCCAGCAGGAGCT137250.1382739740877091No Hit
ACCGTGGAGAGAGAGAAAGAGCAGATGATGCGCGAGAAGGAGGAGTTGAT133760.13475793642238232No Hit
CGGTCAGAAAATGAAGAGTTTGTTGAAGTGGGAAGATTGGGGCCTTCTGA133100.1340930123939824No Hit
GAGGTCAACCTGCAGGCCATGAAGGCCCAGTTCGAGCGGGACCTGCAGGG132400.13338778993961886No Hit
GGAATTGACAATGGCCATGTCCTACGTGAAGGATGACATCTTCCGAATCT130390.13136279403494638No Hit
CCCAGACTACGACTTGTGTAGCGTCTGCGAGGGAAAGGGCTTGCACCGGG129230.13019413968200108No Hit
TGAAAAAGCTGGAGGAGGAGCAGATCATCCTGGAGGACCAGAACTGCAAG129030.12999264755218295No Hit
CATCGAGTGGAACTTCATCGACTTTGGCCTCGACCTGCAGCCCTGCATCG127910.12886429162520127No Hit
GCTGCACTGCGGGCTAAGGAGGAGCTGGAGAGACAGGCGGTGGATCAGAT125300.12623481933107433No Hit
GGCTGCGGAGAACAGGCTCACGGAGATGGAGACGCTGCAGTCTCAGCTCA121940.12284975155012932No Hit
CTCGTGAGGAGATCCTGGCCCAGGCCAAAGAGAACGAGAAGAAGCTGAAG120840.12174154483612948No Hit
CGCACGGAGATGGAGGACCTTATGAGCTCCAAGGATGATGTGGGCAAGAG120670.12157027652578403No Hit
CCAAGCAGCTGAAGGACAAAGCTGATTTCTGCATTATCCACTATGCCGGC120310.12120759069211137No Hit
CGTTCTACATCTCATTCAGGTCAAGCAGGGTCTGGTCCAGCATCCTTTGT120270.12116729226614774No Hit
GAGGAGGCCAAGACCCACGAGGCCCAGATCCAGGAGATGAGGCAGAAGCA120160.12105647159474775No Hit
ATCTAAGAGCCTCGCCAAGCTCAAGAACAAGCATGAGGCAATGATCACTG120120.12101617316878412No Hit
CAAGGACATGTTCCAGGAGACCATGGAGGCCATGAGGATTATGGGCATCC119880.12077438261300232No Hit
GAAGAAGCTGAAGGATGTGCTGCTGCAGGTGGATGACGAGCGGAGGAACG113330.11417551536145774No Hit
CAGAATCCGAAGGGCCAAGCTCTCTGGACCCCTCCCAGGAGGGACCCACA110180.11100201431682179No Hit
AGTTGCAGGAGGAACTGGCAGCCGCGGAGCGTGCCAAGCGCCAGGCCCAG109720.11053858241824002No Hit
GCCCGGGCAGGAATCTGATGACTTTGAGCTGTCTGGCTCTGGAGATCTGG109550.11036731410789459No Hit
GAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAGGAGCTCCAGGCCGCCCT108750.10956134558862199No Hit
GCACGAGATGCCCCCTCACATCTATGCCATCACAGACACCGCCTACAGGA107910.10871507864338573No Hit
GCACATCGACTCGGCCAACAAGAACCGGGACGAAGCCATCAAACAGCTGC106620.10741545440605862No Hit
CTGAATGACAACATCGCCACACTGCTCCACCAGTCCTCTGACAAGTTTGT106000.10679082880362234No Hit
TCCAGGCCCAGATCGCGGAGCTCAAGATGCAGCTGGCCAAGAAAGAGGAG105880.10666993352573145No Hit
CCCCAAGTTCCAGAAGCCCAAGCAGCTGAAGGACAAAGCTGATTTCTGCA105310.1060956809557497No Hit
CCGCACTGAGCACTGCTCTCAGTGAGAAGCGCACGCTGGAGGGCGAGCTG105000.10578336815453156No Hit
TATGAGGAAGAAATCAAGATTCTTACTGATAAACTCAAGGAGGCAGAGAC100520.10126994444660489No Hit
GGCACACCAAGCTCGCATTCCCCAGCCCCTTCGGGCACCTGTCTGAGGGC100110.10085688558047767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)